Serological detection of early pregnancy in cattle and partial characterization of a serum glycoprotein associated with early pregnancy

Klima, F.; Tiemann, Ute; Pitra, Christian; Kauffold, P

doi:10.1016/0165-0378(87)90004-0

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…The steepest decrease of serum levels after abortion is in agreement with Tinneberg et al (1985) estradiol, although its significance in early pregnancy is not yet clear (Ricketts et al, 1980). Our EPAP results in contrast to Klima et al (1987) do not show a close relationship concerning the disappearance rate after removal of fetus. Nevertheless, EPAP as an estrogen sensitive pregnancy glycoprotein and probable EPF carrier may protect the embryo by repelling substances, which could damage them (Powell, 1987;Leipe et al, 1988).…”

Section: Idiseussonsupporting

confidence: 91%

“…For rosette inhibition an anti-human T-lymphocyte globulin obtained from rabbit antiserum (ATG-Fresenius, Fresenius AG, Bad Homburg, FRG) was used. EPAP was estimated as described by Klima et al (1987) and Leipe et al (1988) by means of rocket immunoelectrophoresis using the Sartophor ® electrophoresis system SM 16531 (Sartorius GmbH, Göttingen, FRG).…”

Section: Methodsmentioning

confidence: 99%

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Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone^*)

Hübel¹,

Straube²,

Loh³

et al. 2009

Exp Clin Endocrinol Diabetes

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Serum activities and concentrations, respectively, of early pregnancy factor (EPF), early pregnancy associated protein (EPAP), beta-hCG, estradiol, progesterone and 17-hydroxyprogesterone were estimated in 20 healthy primigravidae within the tenth to twelfth completed gestational week before and after therapeutic abortion. EPF, beta-hCG and estradiol markedly decreased after termination of pregnancy, whereas progesterone and 17-hydroxyprogesterone concentrations moderately fell. EPAP showed no significant alterations in a 24 hours period. Because of its possible role as immunosuppressive substance and its short half-life EPF may be a promising immunobiomarker for disturbances in early pregnancy.

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Section: Idiseussonsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone^*)

Hübel¹,

Straube²,

Loh³

et al. 2009

Exp Clin Endocrinol Diabetes

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“…In ruminants during early pregnancy several protein signals originating from the conceptus and of an antiluteolytic (ewe: Martal et al, 1979;Godkin et al, 1982Godkin et al, , 1984Vallet et al, 1988;Charpigny et al, 1988;cow: Knickerbocker et al, 1986) or immunosuppressive (ewe: Segerson, 1981;cow: Fisher et al, 1985;Klima et al, 1987) nature have been identified. None of those signals has yet been found in the peripheral circulation.…”

Section: Introductionmentioning

confidence: 99%

Pregnancy-specific protein B and progesterone concentrations in French Alpine goats throughout gestation

Humblot¹,

Montigny²,

Jeanguyot³

et al. 1990

Reproduction

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Summary. The 34 French Alpine dairy goats originated from a single flock and were artificially inseminated 44 h after synchronization of oestrus. They were bled daily at the jugular vein from 15 to 27 days after AI. An early pregnancy diagnosis by RIA of progesterone concentration was performed 21 days after AI. In pregnant goats (\ m=ge\1\ m=. \ 5ng progesterone/ml) daily sampling was extended until 30 days after AI and, from those, 9 were bled every 2 weeks until the end of pregnancy and at 50 and 63 days post partum. Pregnancy-specific protein B (PSPB) was also assayed.The kidding rate was 67\m=.\6% (23/34). PSPB concentrations (ng/ml) in pregnant goats were significantly different from those of non-pregnant goats at 24 days after AI (0\m=.\82 \m=+-\0\m=.\18vs 1\m=.\78 \ m=+-\ 0\m=.\19; mean \m=+-\ s.e.m.) and rose to 40 ng/ml at the end of pregnancy. From Day 25 and throughout gestation, females with 2 fetuses had higher PSPB concentrations than did those with a single fetus (P < 0\m=.\05). In the 2 goats exhibiting late embryonic mortality according to progesterone concentrations, one had a PSPB profile very similar to those of pregnant goats until 30 days while the other did not show any elevation of PSPB concentration. It is concluded that PSPB profiles in goats are similar to those found in cows throughout pregnancy and that PSPB RIA may be useful for pregnancy diagnosis or diagnosis of late embryonic mortality.

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Human early pregnancy factor: serum concentrations before and after therapeutic abortion in comparison with ?-hCG and an early pregnancy associated protein

Straube

Hübel

Loh

et al. 1989

Arch Gynecol Obstet

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Serum concentrations of the early pregnancy factor (EPF), beta-hCG and the early pregnancy associated protein (EPAP) were measured in 12 patients before and after therapeutic abortion for social-medical reasons. Detection of EPF was performed by the rosette-inhibition assay, beta-hCG quantification by radioimmunoassay and EPAP measurements by rocket immunoelectrophoresis using a monospecific polyclonal rabbit antiserum. The disappearance rate of EPF after termination of pregnancy was closely correlated with the decrease of beta-hCG concentrations (rpbis = 0.71) and loosely correlated with the decrease of EPAP concentrations (rPbis = 0.43). No correlation has been found between hCG and EPAP values.

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Serological detection of early pregnancy in cattle and partial characterization of a serum glycoprotein associated with early pregnancy

Cited by 5 publications

References 17 publications

Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone^*)

Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone^*)

Pregnancy-specific protein B and progesterone concentrations in French Alpine goats throughout gestation

Human early pregnancy factor: serum concentrations before and after therapeutic abortion in comparison with ?-hCG and an early pregnancy associated protein

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Serological detection of early pregnancy in cattle and partial characterization of a serum glycoprotein associated with early pregnancy

Cited by 5 publications

References 17 publications

Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone*)

Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone*)

Pregnancy-specific protein B and progesterone concentrations in French Alpine goats throughout gestation

Human early pregnancy factor: serum concentrations before and after therapeutic abortion in comparison with ?-hCG and an early pregnancy associated protein

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Product

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Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone^*)

Human Early Pregnancy Factor and Early Pregnancy Associated Protein before and after Therapeutic Abortion in Comparison with ß-hCG, Estradiol, Progesterone and 17-Hydroxyprogesterone^*)