Serological Diagnosis of Candidal Infections

Taschdjian, Claire L.; Seelig, Mildred S.; Kozinn, Philip J.

doi:10.3109/10408367309151683

Cited by 73 publications

(50 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Complex antigen extracts have been prepared in various ways by different authors, and several parameters can be varied during the procedure (6,85). That significant differences exist between products made by different investigators has been directly shown by Faux et al (30) and Axelsen (7; unpublished data), but this important problem has not been sufficiently elucidated experimentally.…”

Section: The Antigens Of C Albicansmentioning

confidence: 99%

“…This method is evidently well suited for rapid detection of microbial antigens or precipitins (97), provided suitable reagents (antigens/antibodies) are available and qualitative test results (positive/negative) give sufficient information. However, one of the major problems in diagnostic Candida serology is to define suitable test antigens (85). It was therefore suggested (9) that crossed IE with intermediate gel should be adopted as a screening method by special research laboratories in order to provide specific guidelines for the production of such antigens.…”

Section: Immurioprecipitat/on Techniquesmentioning

confidence: 99%

“…Mannan is soluble in water and reacts in precipitation assays (24,38). Purified mannan has been used by only a few authors for detection of human antibodies (24), whereas more or less crude polysaccharide extracts have been more frequently used (85).…”

Section: The Antigens Of C Albicansmentioning

confidence: 99%

“…According to Taschdjian et al, (85), such preparations are the best available reagents for gel precipitation tests for diagnosis of systemic candidiasis. The number of antigens that has been detected in such extracts by use of rabbit antisera ranges from 5 to 7 by double diffusion in gel (16,88), through 15 to 17 by classical IE (17,18), to 68 to 78 by crossed IE (3,6).…”

Section: The Antigens Of C Albicansmentioning

confidence: 99%

“…The first three methods have demonstrated antibodies in a considerable proportion of healthy individuals, and these methods have therefore been considered to be of minor diagnostic value, although high or rising agglutinin and fluorescent antibody titers have been reported to be of some significance for diagnosis of systemic candidiasis (85). Candida precipitins are also present in healthy individuals, but gel precipitation methods offer better possibilities for identification of diagnostically important antigens.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Analysis of Human Candida Precipitins by Quantitative Immunoelectrophoresis

Axelsen

1976

Scand J Immunol

View full text Add to dashboard Cite

Candida immunochemistry was the first microbial field to be investigated by quantitative immunoelectrophoresis (QIE), and the main purpose of this review is to discuss the methodology of human Candida precipitin analysis in the light of this development (3-7, 9, H, 12, 83). QIE techniques are now in use for study of Psei/domonas aeruginosa (39-47), mycobacteria (10,25,26, 58,59,75, 103), herpes simplex virus (53,(93)(94)(95)(96), Aclinomyces israelii (50, 51), and other microbial antigens and antibodies (47,48,54, 55,71, 84,86,98). Application and development of QIE methodology for study of raicrobial immunochemistry has been very fruitful, as shown by the discovery of a large number of new microbial antigens and a corresponding complexity of the human antibody response. (85); their conclusion was that serodiagnosis of deep Candida infection is urgently needed and appears promising, but further progress is dependent on development of suitable standardized antigens and standardized test methods. There is also an urgent need for standardized Candida antigens for evaluation of human T-cell effector action and for evaluation of immediate and Arthus-type hypersensitivity against Candida antigens. The need comprises antigens for use both in vitro and in vivo. The Standardization Committee of the International Union of Immunoiogical Societies has therefore recently established a 'subcommittee for Candida' (Chairman: Prof. J, R. Hobbs, Westminster Medical School, London) to recommend how such standards should be prepared. The present review is confined to discussing the immunochemistry of C. albkans and human precipitin analysis. Further, some implications of recent immunochemical progress for other immunoiogical tests are briefly outlined.Most studies of human Candida antibodies have been performed to evaluate the possibilities for serodiagnosis of candidiasis. From a clinical standpoint the deep-seated or systemic Candida infection offers special difficulties. These infections are claimed to be almost impossible to diagnose on clinical grounds (78, 85), and pathognomonic isolation of Candida species from normally sterile sites often fails or is misinterpreted as to diagnostic significance (85). The incidence of systemic candidiasis is rising, the mortality rate is high in untreated patients, and treatment with antifungal agents such as amphotericin B should be restricted to well-documented cases because of toxic side effects (85). Systemic candidiasis is a significant problem in transplanted patients, in patients with leukemia, and in patients undergoing open-heart surgery

show abstract

Section: The Antigens Of C Albicansmentioning

confidence: 99%

Section: Immurioprecipitat/on Techniquesmentioning

confidence: 99%

Section: The Antigens Of C Albicansmentioning

confidence: 99%