Serological examination of a rhabdovirus isolated from snakehead (Ophicephalus striatus) in Thailand with ulcerative syndrome

Ahne, W.; Jørgensen, P. E. V.; Olesen, Niels Jørgen; Wattanavijarn, Wattana

doi:10.1111/j.1439-0426.1988.tb00562.x

Cited by 29 publications

(11 citation statements)

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“…In the last 10 years, virologists isolated and identified the following rhabdoviruses from aquaculture fish: Siniperca chuatsi rhabdovirus [104], Scophthalmus maximus rhabdovirus [105,106], Paralichthys olivaceus rhabdovirus [107], Monopterus albus rhabdovirus [108], snakehead rhabdovirus [109,110], Hirame rhabdovirus [111], and pike fry rhabdovirus [112]. Spring viremia of carp virus (SVCV), an earlier identified rhabdovirus, causes infectious hemorrhagic septicemia in common carp (Cyprinus carpio) [102,113].…”

Section: Rhabdovirus Genomesmentioning

confidence: 99%

Virus genomes and virus-host interactions in aquaculture animals

Zhang

Gui

2015

Sci. China Life Sci.

172

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Over the last 30 years, aquaculture has become the fastest growing form of agriculture production in the world, but its development has been hampered by a diverse range of pathogenic viruses. During the last decade, a large number of viruses from aquatic animals have been identified, and more than 100 viral genomes have been sequenced and genetically characterized. These advances are leading to better understanding about antiviral mechanisms and the types of interaction occurring between aquatic viruses and their hosts. Here, based on our research experience of more than 20 years, we review the wealth of genetic and genomic information from studies on a diverse range of aquatic viruses, including iridoviruses, herpesviruses, reoviruses, and rhabdoviruses, and outline some major advances in our understanding of virus-host interactions in animals used in aquaculture.

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Section: Rhabdovirus Genomesmentioning

confidence: 99%

Virus genomes and virus-host interactions in aquaculture animals

Zhang

Gui

2015

Sci. China Life Sci.

172

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“…Most fish rhabdoviruses have been analyzed and compared in terms of their morphology, cytopathogenicity, serological relatedness and structural polypeptide composition (Ahne et al 1988, Frerichs 1989, Kasornchandra et al 1991, 1992, Lilley & Frerichs 1994, Granzow et al 1997. Recently, reverse transcriptase-polymerase chain reaction (RT-PCR) assay has been developed and applied to detecting fish rhabdovirus IHNV and VHSV in fixed and embedded fish tissue by Chiou et al (1995) and in organ samples and cultured cells by Miller et al (1998).…”

Section: Discussionmentioning

confidence: 99%

“…In cultured penaeid shrimp, the yellow-head virus (YHV) that caused massive losses in Thailand and elsewhere throughout Asia was initially described as a baculovirus, but was recently reclassified as a rhabdovirus (Nadala et al 1997). Additionally, a yellow-head-like virus was also found to be associated with mortalities of cultured Penaeus monodon in Australia (Spann et al 1997).Most fish rhabdoviruses have been analyzed and compared in terms of their morphology, cytopathogenicity, serological relatedness and structural polypeptide composition (Ahne et al 1988, Frerichs 1989, Kasornchandra et al 1991, 1992, Lilley & Frerichs 1994, Granzow et al 1997. Recently, reverse transcriptase-polymerase chain reaction (RT-PCR) assay has been developed and applied to detecting fish rhabdovirus IHNV and VHSV in fixed and embedded fish tissue by Chiou et al (1995) and in organ samples and cultured cells by Miller et al (1998).…”

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confidence: 99%

Isolation of a lethal rhabdovirus from the cultured Chinese sucker Myxocyprinus asiaticus

Zhang

Li²,

Gui

2000

Dis. Aquat. Org.

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A rhabdovirus was found to be associated with a lethal hemorrhagic disease in the cultured Chinese sucker Myxocyprinus asiaticus Bleeker. The rhabdovirus was amplified and isolated from the infected GCO (grass carp ovary) cells. In ultrathin sections of liver cells from the diseased fish, the virus particles exhibited the characteristic bacilliform morphology, and budded through vesicle membranes of the infected cells. The isolated rhabdovirus particles were found to have a bacilliform morphology with 2 rounded ends rather than a typical flat base. The virus particles were measured and ranged in size from 150 to 200 nm in length and 50 to 60 nm in diameter. Most other characteristics, including their size, extensive virus infectivity to fish cell lines, strong cytopathogenic effects, stability at high temperatures, vesicle formation in infected cells, structure protein electrophoretic patterns and the presence of an RNA genome, very closely resembled those of other fish rhabdoviruses. At present it is not known if this is a novel virus species or if it is an isolate of a known fish rhabdovirus. Until a confirmed identification can be made, we will temporarily refer to this virus as Chinese sucker rhabdovirus (CSRV). KEY WORDS: Rhabdovirus · Myxocyprinus asiaticus · Fish viral disease · Electron microscopy · Chinese sucker rhabdovirus (CSRV) Resale or republication not permitted without written consent of the publisherDis Aquat Org 42: [1][2][3][4][5][6][7][8][9] 2000 that the disease might be related to viral infection. We therefore collected diseased fish showing typical symptoms and made an attempt to isolate the virus. Through a series of observations and analysis, a rhabdovirus was identified as the pathogen associated with the disease of sucker fish. Here, we report the isolation process of the rhabdovirus. MATERIALS AND METHODSProcessing of diseased fish samples. A total of 35 diseased juveniles were collected from the diseased population cultured in one pond. These juveniles, weighing about 5 to 10 g, were used to prepare tissue extracts. Liver, spleen, kidney and muscle were sampled from the diseased fish. The samples were ground in a mortar and diluted 1:10 in phosphate buffered saline (PBS) containing antibiotics (100 IU penicillin ml -1 , 100 µg streptomycin ml -1 ). The extract was stored overnight at -20°C. Then, the supernatant was collected by centrifugation at 2000 × g, and filtered through a 300 to 450 nm filter membrane. The filtered supernatant was used as the viral isolate for infecting cell lines, and stored at -80 or -20°C.Cell lines to be used. A total of 8 cell lines, including GCK (grass carp kidney), FHM (fathead minnow), CAB (Carassius auratus blastula embryos), CLC (leucocytes of common carp), EPC (epithelioma papulosum cyprini), GCO (grass carp ovaries), GCF (grass carp fins), and GRO (Gobiocypris rarus ovary), were used for viral sensitivity tests (Zhang 1997). The cells were grown in TC 199 medium with 10% fetal bovine serum and antibiotics (100 IU penicillin ml ...

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“…A primary viral infection has always been considered likely, and virus-like particles of varying morphology were first observed in the tissues of diseased fish in Thailand in 1982 (WAITANAVIJARN et al 1983). Since then, a birnavirus (SGV) has been isolated from diseased sand gobies (Oxyeleotris mannoratus) (HEDRICK et al 1986), a rhabdovirus from snakehead fish (Ophicephalus striatus) and freshwater eel (Fluta alba) (FRE-RICHS et al 1986;AHNE et al 1988) and a second birnavirus, IPN virus serotype Sp, from snakehead fish and barb (Harnpakz dispar) (WATTANAVIJARN et al 1988). None of these viruses, however, has yet been established as a primary causal agent of the disease syndrome.…”

Section: Us Copyright Clearance Center Code Statementmentioning

confidence: 98%

Efficacy of chemical disinfectants against snakehead rhabdovirus

Frerichs

1990

J Appl Ichthyol

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The susceptibility of snakehead rhabdovirus to treatment at 20 "C with 5 commercially available disinfectants was examined. No reduction in virus infectivity occurred followin exposure to 5 ppm malachite green for 6 hours. Treatment of infective ceII culture fluids with 2 % formalin resulted in > 99.9% reduction in virus titre within 5 minutes and complete inactivation within 30 minutes, but a negli ible loss in infectivity after exposure to 0.025% formalin for 1 hour. Suspensions of virus in distded water were completely inactivated within 5 minutes by 12.5 ppm chlorine, 50 pm iodine, or a 1 : 2000 dilution of a peroxygen disinfectant. In the presence of serum in infective cei culture fluids, however, > 50 pm chlorine was required to inactivate the agent and no measurable reduction in infectivity was ogserved following treatment with 500 ppm iodine for 30 minutes.

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Serological examination of a rhabdovirus isolated from snakehead (Ophicephalus striatus) in Thailand with ulcerative syndrome

Cited by 29 publications

References 1 publication

Virus genomes and virus-host interactions in aquaculture animals

Virus genomes and virus-host interactions in aquaculture animals

Isolation of a lethal rhabdovirus from the cultured Chinese sucker Myxocyprinus asiaticus

Efficacy of chemical disinfectants against snakehead rhabdovirus

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