Serological grouping of streptococci by a slide coagglutination method.

Finch, R G; Phillips, I. A.

doi:10.1136/jcp.30.2.168

Cited by 18 publications

(9 citation statements)

References 11 publications

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“…Numerous investigations have shown that coagglutination testing, particularly with the Phadebact Streptococcus Test, affords the clinical microbiologist a rapid and reliable method to serogroup beta-hemolytic streptococci (10,16,17). The present investigation confirms the diagnostic potential of this method and shows the capabilities of the relatively new SeroSTAT and Streptex latex agglutination tests for the serogrouping of beta-hemolytic streptococci.…”

Section: Discussionsupporting

confidence: 79%

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Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

Slifkin¹,

Pouchet-Melvin²

1980

J Clin Microbiol

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Three beta-streptococci serogrouping kits, Phadebact, SeroSTAT, and Streptex, were evaluated as to their sensitivity, accuracy, and suitability as methods for serogrouping streptococci in a clinical microbiology laboratory. The majority of the primary isolates examined by the various methods associated with each of the three kits were correctly identified. The Streptex direct mixed-culture procedure was more often associated with the observation of cross-reactivity than with the direct procedures of the other two kits which did not employ mixed growth cultures. Furthermore, the Streptex kit was associated with more falsenegative responses than those determined by the other two kits under evaluation. These results appeared to be due to the relatively poor sensitivity of the Streptex grouping reagents. The Streptex test procedures required more labor than the other kit procedures, requiring a 1-h enzymatic extraction step for the release of the group antigens. The SeroSTAT kit provided only a direct procedure and, thus, is limited in its application. The Phadebact procedures were the most versatile by providing not only a direct and a 24-h grouping procedure, but also by including a 4-h method that may be employed as required by the clinical microbiologist.

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Section: Discussionsupporting

confidence: 79%

“…c +, Weak response; -, no response. d Other bacteria tested: Citrobacter diversus (5), Enterobacter cloacae (10), Escherichia coli (10), P. aeruginosa (10), Serratia liquefaciens (10), viridans streptococci (8).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

Slifkin¹,

Pouchet-Melvin²

1980

J Clin Microbiol

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“…Surface fibrillar structures (M. Jacques et al, submitted for publication) as weil as hemagglutinins (17) of S. suis have recently been described and would support this theory. Also, nonspecific agglutinations among different streptococci were described earlier (11,12). Not only may the nonspecific agglutination described here with reference strains occur, but other agglutinations among field isolates (unpublished observations) which would make absorption of antisera useless may also occur.…”

Section: Resultssupporting

confidence: 53%

Description of 14 new capsular types of Streptococcus suis

et al. 1989

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Fourteen new capsular types of Streptococcus suis (types 9 to 22) are described. All reference strains are morphologically and biochemically similar to types previously described. Reference strain types 9 to 13, 15, 16, and 22 were isolated from diseased pigs, whereas types 17 to 19 and 21 came from clinically healthy pigs; type 14 was isolated from a human case of meningitis, and type 20 was isolated from a diseased calf. The group T streptococcus of de Moor has been included in the typing system as type 15. Two-way cross-reactions between types 6 and 16 and a one-way cross-reaction between types 2 and 22 have been demonstrated. In addition, several cross-reactions probably not due to capsular material were detected among different types by using the coagglutination test. This test should not be used alone; weak or multiple positive reactions must be confirmed by the capsular reaction test or the capillary precipitation test. Streptococcus suis is an important swine pathogen isolated in almost all countries where the pig industry is developed. It has been associated mainly with bronchopneumonia and meningitis and less frequently with endocarditis, arthritis, and other infections (2, 10, 18, 19, 25, 26, 28). It is also known to cause meningitis in humans (1, 6, 30). Originally defined as Lancefield groups R, S, RS, and T by de Moor (7), strains of S. suis were later shown to share antigens with group D streptococci (8). The capsule on S. suis cells suggested a type-specific antigenicity, and groups

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“…Methods of bacterial agglutination by specific antisera are frequently used for their detection (CARTER and RYAN 1975, FINCH and PHILLIPS 1977, KHOURY et al 1973, KoDAMA and ISHIMOTO 1976, MASSEY and MANGIAFICO 1974, SATO and DiENA 1974. The direct agglutination procedure in tubes or on slides is simple and relatively sensitive.…”

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confidence: 99%

Immunological Methods for the Detection of a Coryneform Isolated from Ratoon Stunted Sugarcane

Oellermann

Pillay

1987

Journal of Phytopathology

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A coryneform bacterium, isolated from ratoon stunted sugarcane, has been obtained in pure culture. Its identification by immunological means was investigated. Optimum conditions for the light microscopic observation of bacterial agglutination by specific antiserum, immunofluorescence and the microcapillary haemagglutination of IgG‐sensitized erythrocytes by the bacterium were established. All three assay procedures are simple and rapid. By comparison, more samples can be handled at any one time bythe microcapillary haemagglutination method. Based on bacterial agglutination, the antiserum is specific for the coryneform isolated from ratoon‐stunted sugarcane.

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Serological grouping of streptococci by a slide coagglutination method.

Cited by 18 publications

References 11 publications

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci

Description of 14 new capsular types of Streptococcus suis

Immunological Methods for the Detection of a Coryneform Isolated from Ratoon Stunted Sugarcane

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