Serological Identification of an Ir-Region Product

Mouse spleen or thymus lymphocytes incubated with monospecific H-2 or Ia alloantisera and then coated with a xenogeneic antimouse Ig serum become specifically resistant to the alloantiserum (and complement) they have been incubated with. This so called "lysostrip method" was used to investigate the molecular interrelationships of antigens in the mouse lymphocyte membrane. The results of this investigation confirm that H-2K and H-2D antigens are carried by two distinct populations of molecules. They provide evidence that the Ia antigens move in the membrane independently of both H2-K and H-2D antigens; and finally they demonstrate absence of any physical linkage between Ig receptors in B cells, on the one hand, and Ia, H-2K, and H-2D molecules on the other hand.

Section: Serial Nomentioning

confidence: 99%

Section: Serial Nomentioning

confidence: 99%

Induction of resistance to antibody-mediated cytotoxicity. H-2, Ia, and Ig antigens are independent entities in the membrane of mouse lymphocytes.

Hauptfeld¹,

Hauptfeld²,

Klein³

1975

“…In contrast to the wide tissue distribution of the H-2K and H-2D major histocompatibility antigens, the Ia antigens have a restricted distribution with principal expression on subpopulations of T and B lymphocytes (2)(3)(4)(5)(6)(7)(8). The mapping of such important immune functions as mixed lymphocyte reaction, graft versus host reactivities, immune response control (It genes), and cell-cell interaction determinants to the I region has stimulated efforts to identify possible relationships between Ia determinants and these immune functions (9)(10)(11)(12)(13)(14).…”

mentioning

confidence: 99%

Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells.

Niederhuber¹,

Frelinger²,

Dine³

et al. 1976

Genes mapping in the I region of the H-2 complex control a system of lymphocyte alloantigens (Ia) which are expressed on a subpopulation of T cells and on most B cells. Specific anti-Ia serum in the presence of rabbit complement removed the splenic T-cell subpopulation responsive to Con-A, but did not affect the response to phytohemagglutinin (PHA) or Leucoagglutinin. Antibodies specific for Ia, H-2K, or H-2D membrane antigens were used without complement to pretreat spleen cells. These antibody pretreated cells responded normally to Con-A and PHA.

“…Studies to define the relationship between the 2/13 locus and the ABCD locus of Sato and de Weck are now in progress. Alternatively, as outlined in the preceeding paper (3), the antigens recognized by the anti-2 and anti-13 sera may be the product of MHC genes which are not formally equivalent to the genes controlling the major serologic specificities in the mouse (the D and K genes) but rather to genes mapping within the Ir region of mice (13,14). According to this thesis, the cytolytic antibodies in the anti-GA+PLL-2-13 + sera would be directed not at products of these critical Irrelated H genes but rather at products of other genes in the MHC.…”

Section: Discussionmentioning

confidence: 99%

Alloantiserum-Induced Inhibition of Immune Response Gene Product Function

Shevach

Green

Paul

1974

Alloantisera prepared in strain 13 guinea pigs by immunization with strain 2 lymphoid cells markedly inhibit the activation of (2 X 13)F1 peritoneal exudate lymphocytes by the 2,4-dinitrophenyl (DNP) derivative of a copolymer of L-glutamic acid and L-lysine (GL) 1 (1). Responses to DNP-GL and to the closely related antigen, DNP-poly-L-lysine (DNP-PLL), depend upon the presence of an immune response (Ir) gene linked to the major histocompatibility complex (MHC) controlling the strain 2 ailoantigen(s) (2). This finding, with appropriate specificity controls, has led us to propose that alloantisera inhibit antigen recognition by thymus-dependent (T) lymphocytes through interference with the activity of Ir gene products (1).In the first paper (3) of this series, we examined the effect of absorption of the 13 anti-2 serum with different populations of immunocompetent cells. We were unable to demonstrate a clear dissociation between the ability of a cell population to remove the cytotoxic activity of the anti-2 serum for lymphocytes and the ability of that population to remove the capacity of the anti-2 serum to inhibit T-cell proliferation in response to DNP-GL. We concluded that the 13 anti-2 serum contains antibodies directed against cell surface structures present on both thymus-derived (T) lymphocytes and bone marrow-derived (B) lymphocytes, because L2C leukemia cells, a pure population of malignant B lymphoid cells, were able to remove both the cytotoxic capacity and the T-cell inhibitory capacity from the anti-2 serum. Indeed, the relevant antigens appear to be present in larger amount on B cells than on T ceils. It is possible that the anti-2 serum contains different populations of antibodies, some of which are reactive with strain 2 histocompatibility (H) antigen(s) and other which are t Abbreviations used in this paper: CFA, complete Freund's adjuvant; GA, a copolymer of L-glutamic acid and L-alanine; GL, a copolymer of L-glutamic acid and L-lysine; GT, a copolymer of L-glutamic acid and L-tyrosine; H, histocompatibility; Ir, immune response; MHC, major histocompafibility complex; NGPS, normal guinea pig serum; PEC, peritoneal exudate ceils; PELs, peritoneal exudate lymphocytes; PHA, phytohemagglutinin; PLL, poly-Llysine; PPD, purified protein derivative of tuberculin; SI, stimulation index.