Serological Identification of Endothelial Antigens Predominantly Recognized in Kawasaki Disease Patients by Recombinant Expression Cloning

Kaneko, Miho; Ono, Toshiro; Matsubara, Tomoyo; Yamamoto, Yukiyo; Ikeda, Hitoshi; Yoshiki, Takashi; Furukawa, Susumu; Nakayama, Eiichi

doi:10.1111/j.1348-0421.2004.tb03472.x

Cited by 26 publications

(25 citation statements)

References 42 publications

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“…The authors used a proteomic approach and human dermal microvascular EC as protein source [20]. Recently, another approach based on serological analysis of recombinant cDNA expression library has been developed to identify target antigens in Kawasaki disease [21].…”

Section: Detection Of Anti-ec Autoantibodies and Identification Of Thmentioning

confidence: 99%

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Antiendothelial Cells Autoantibodies in Vasculitis-Associated Systemic Diseases

Guilpain

Mouthon

2008

Clinic Rev Allerg Immunol

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Antiendothelial cell antibodies (AECA) have been detected in healthy individuals, as well as in autoimmune and systemic inflammatory diseases, including systemic vasculitides. AECA have been reported in large vessel vasculitides such as giant cell arteritis and Takayasu arteritis; medium-sized vessel vasculitides, such as polyarteritis nodosa related to hepatitis B virus infection and Kawasaki disease; and small-sized vessel vasculitides, such as Wegener's granulomatosis, microscopic polyangiitis, and Henoch-Schonlein purpura. In Takayasu arteritis and antineutrophil cytoplasm antibody-positive vasculitides, AECA have been reported to correlate with disease activity. A cell-based enzyme-linked immunosorbent assay (ELISA) using cultured human umbilical vein endothelial cells (HUVEC) represent one of the reference techniques for AECA detection, although flow cytometry and immunobloting have also been proposed. AECA might contribute to the pathogenesis of systemic vasculitides and vasculitis-associated diseases through (1) activation of endothelial cells (EC), (2) direct cytotoxic effect due to complement-dependent cytotoxicity or indirect cytotoxic effect secondary to antibody-dependent cytotoxicity, (3) induction of coagulation, (4) induction of apoptosis through the binding of phospholipids or heat-shock protein 60, and (5) induction of EC activation. None of the identified target antigens of AECA is specific for EC, and EC-specific target antigens of AECA remain to be identified in systemic vasculitides.

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Section: Detection Of Anti-ec Autoantibodies and Identification Of Thmentioning

confidence: 99%

“…AECA have also been detected in Kawasaki disease [27][28][29]. Using serological analysis of recombinant cDNA expression library, Kaneko et al identified tropomyosin and T-plastin, a cytoskeletal protein, as preferential target antigens for AECA from patients with Kawasaki disease [21].…”

Section: Aeca In Systemic Vasculitidesmentioning

confidence: 99%

Antiendothelial Cells Autoantibodies in Vasculitis-Associated Systemic Diseases

Guilpain

Mouthon

2008

Clinic Rev Allerg Immunol

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“…Recognition of autoantigens by antiendothelial cell antibodies has been reported [8,9]. We previously demonstrated a marked elevation of a B-cell-activating factor belonging to the TNF family (BAFF) [10] before intravenous immunoglobulin therapy (IVIG), and its steep drop after the therapy [11], which suggest that B-cell activation in the acute phase might be involved in the pathogenesis of KD.…”

Section: Introductionmentioning

confidence: 99%

Persistent Endothelial Damage after Intravenous Immunoglobulin Therapy in Kawasaki Disease

Sakurai¹,

Takatsuka²,

Onaka³

et al. 2014

Int Arch Allergy Immunol

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Background: Kawasaki disease (KD) is an acute systemic vasculitis of unknown etiology. Although endothelial cell damage associated with vasculitis might lead to the hypercoagulability that is involved in coronary artery disease, the changes in coagulation after intravenous immunoglobulin therapy (IVIG) have not been well investigated in KD. The aims of this study were to address the changes in coagulation before and after IVIG in KD, and to further elucidate the coagulation-inflammation axis, with special attention to endothelial damage. Methods: We retrospectively collected the laboratory data before and after IVIG in 26 pediatric KD patients treated at the Nara Prefecture Western Medical Center between May 2010 and April 2012. Prothrombin time (PT), activated partial thromboplastin time (APTT) and levels of fibrin/fibrinogen degradation products (FDP) and D-dimer were assessed as coagulation markers. Fibrinogen, ferritin, serum amyloid A, procalcitonin and urine F2 microglobulin were assessed as inflammation markers. Thrombomodulin, antithrombin, factor VIII activity (FVIII:C), and von Willebrand factor antigen (VWF:Ag) were used to assess endothelial damage. Results: Prolonged PT and APTT before IVIG were significantly shortened after IVIG, and elevated levels of FDP and D-dimer were significantly decreased. Elevated levels of inflammation markers had decreased significantly after IVIG, but levels of FVIII:C and VWF:Ag remained high, even after IVIG. Conclusions: Ameliorated inflammation by IVIG might improve the hypercoagulable state. Nevertheless, our results suggest that endothelial damage might be prolonged in IVIG-treated patients. Control of endothelial damage in KD is critical. i 2014 S. Karger AG, Basel

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“…Kaneko et al [63] successfully applied serological analyses to a recombinant cDNA expression library in KD patients and identified some EC antigens as targets of AECA, including tropomyosin and T-plastin.…”

Section: Expert Opinionmentioning

confidence: 98%

“…To date, considerable efforts have been made for the identification of AECA autoantigens. As shown in [63] and DNA topoisomerase I [64].…”

Section: Induction Of Coagulationmentioning

confidence: 99%

Anti-endothelial cell antibodies (AECA) in patients with systemic vasculitis: our research using proteomics

Karasawa

Yudoh

Ozaki

et al. 2010

Expert Opinion on Biological Therapy

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Serological Identification of Endothelial Antigens Predominantly Recognized in Kawasaki Disease Patients by Recombinant Expression Cloning

Cited by 26 publications

References 42 publications

Antiendothelial Cells Autoantibodies in Vasculitis-Associated Systemic Diseases

Antiendothelial Cells Autoantibodies in Vasculitis-Associated Systemic Diseases

Persistent Endothelial Damage after Intravenous Immunoglobulin Therapy in Kawasaki Disease

Anti-endothelial cell antibodies (AECA) in patients with systemic vasculitis: our research using proteomics

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