Serological properties and intrinsic antibiotic resistance of soybean bradyrhizobia isolated from Thailand

Yokoyama, Tadashi; Ando, Sugihiro; Tsuchiya, Kenichi

doi:10.1080/00380768.1999.10409366

Cited by 14 publications

(8 citation statements)

References 33 publications

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“…A cluster analysis of strains analysed based on the phenotypic data showed that group Ia strains are more similar to each other than to strains of other species (Fig. S4).In addition to the four strains from this study, several others from our previous studies, all symbionts of soybean, also fitted into group Ia (Chueire et al, 2003;Germano et al, 2006;Menna et al, 2009) (Table S5).Although strain USDA 6T was not included in many studies from Japan and Thailand, undoubtedly several isolates from soybean also fit into group Ia (Minamisawa, 1989(Minamisawa, , 1990Yokoyama et al, 1999). Other group Ia strains from the pioneer studies of Hollis et al (1981), Huber et al (1984, Kuykendall et al (1992) and van Berkum & Fuhrmann (2001) are listed in Table S5, together with strains from other recent studies.…”

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confidence: 94%

“…T was not included in many studies from Japan and Thailand, undoubtedly several isolates from soybean also fit into group Ia (Minamisawa, 1989(Minamisawa, , 1990Yokoyama et al, 1999). Other group Ia strains from the pioneer studies of Hollis et al (1981), Huber et al (1984, Kuykendall et al (1992) and van Berkum & Fuhrmann (2001) are listed in Table S5, together with strains from other recent studies.…”

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confidence: 99%

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Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov.

Delamuta

Ribeiro

Ormeño‐Orrillo

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

233

122

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Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov. Embrapa Meio Ambiente, C.P. 69, 13820-000 Jaguariú na, Sã o Paulo, BrazilBradyrhizobium japonicum was described from soybean root-nodule bacterial isolates. Since its description, several studies have revealed heterogeneities among rhizobia assigned to this species. Strains assigned to B. japonicum group Ia have been isolated in several countries, and many of them are outstanding soybean symbionts used in inoculants worldwide, but they have also been isolated from other legume hosts. Here, we summarize published studies that indicate that group Ia strains are different from the B. japonicum type strain USDA 6 T and closely related strains, and present new morphophysiological, genotypic and genomic evidence to support their reclassification into a novel species, for which the name Bradyrhizobium diazoefficiens sp. nov. is proposed. The type strain of the novel species is the well-studied strain USDA 110 T (5IAM Biological fixation is the main source of nitrogen for natural and agricultural ecosystems. In agriculture, the symbioses of nitrogen-fixing bacteria, collectively known as rhizobia, with crops belonging to the family Leguminosae (5Fabaceae) are the most studied. Relatively high contributions to nitrogen nutrition have been demonstrated in pulses, fodders, green manures and trees (Ormeño-Orrillo et al., 2013). Members of the genus Bradyrhizobium constitute an important group of rhizobia, some of which form symbioses with economically important crops, such as soybean [Glycine max (L.) Merr.].A first systematic classification of rhizobia was proposed by Fred et al. (1932), based on the cross-inoculation concept with respect to the host legume, resulting in six species of Rhizobium: Rhizobium meliloti, R. trifolii, R. phaseoli, R. lupini, R. leguminosarum and R. japonicum, the last of which is a symbiont of soybean. Fifty years later, the taxonomy was redefined using numerical criteria, including several morphophysiological and genetic properties. A new genus was created, Bradyrhizobium, to accommodate rhizobia with the typical property of slow growth rates in culture media, with only one defined species, Bradyrhizobium japonicum (Jordan, 1982). It is noteworthy that the division of rhizobia into fast and slow growers in vitro had been proposed earlier by Löhnis & Hansen (1921).It has been suggested that Bradyrhizobium is the ancestor of the alpha-rhizobia, probably originating in the tropics (Lloret & Martínez-Romero, 2005;Norris, 1965;Provorov & Vorob'ev, 2000). However, despite its evolutionary position, significantly fewer species have been described in Bradyrhizobium in comparison with Rhizobium. One possible explanation is that the 'backbone' of modern taxonomy relies on the analysis of 16S rRNA gene sequences (Woese, 1987), which are highly conserved inAbbreviations: DDH, DNA-DNA hybridization; ITS, intergenic transcribed spacer; MLSA, multilocus sequencing analys...

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confidence: 94%

mentioning

confidence: 99%

Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov.

Delamuta

Ribeiro

Ormeño‐Orrillo

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

233

122

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“…These limitations are addressed with the use of MLST to determine GT. The method not only can be applied to soybean strains that have low compatibility with antisera raised against the 17 serogroups (Thompson et al 1991;Yokoyama et al 1999) but also facilitates the comparison of Fig. 3.…”

Section: Limitations Of Serology That Can Be Addressed Using Mlstmentioning

confidence: 99%

“…For example, serological methods have been used extensively in the study of symbiosis, inoculation success, and rhizobial ecology (Berg et al 1988;Boonkerd et al 1978;Caldwell 1969;Caldwell and Hartwig 1970;Caldwell and Vest 1968;Caldwell and Weber 1970;Cregan and Keyser 1986;Damirgi et al 1967;Ellis et al 1984;Fuhrmann 1990;Ham et al 1971;Johnson et al 1965;Kapusta and Rouwenhorst 1973;Keyser and Cregan 1987;Kvien et al 1981;Means et al 1964;Mpepereki and Wollum 1991;Schmidt et al 1968; Thompson et al 1991;Vest et al 1971;Weber et al 1989;Yokoyama et al 1999).…”

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confidence: 98%

Development and Application of a Multilocus Sequence Analysis Method for the Identification of Genotypes Within Genus Bradyrhizobium and for Establishing Nodule Occupancy of Soybean (Glycine max L. Merr)

Berkum¹,

Elia²,

Song³

et al. 2012

MPMI

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A multilocus sequence typing (MLST) method based on allelic variation of seven chromosomal loci was developed for characterizing genotypes (GT) within the genus Bradyrhizobium. With the method, 29 distinct multilocus GT were identified among 190 culture collection soybean strains. The occupancy of 347 nodules taken from uninoculated field-grown soybean plants also was determined. The bacteroid GT were either the same as or were closely related to GT identified among strains in the culture collection. Double-nodule occupancy estimates of 2.9% were much lower than values published based on serology. Of the 347 nodules examined, 337 and 10 were occupied by Bradyrhizobium japonicum and B. elkanii, respectively. The collection strains within the species B. japonicum and B. elkaniialso were compared with Bradyrhizobium cultures from other legumes. In many cases, the observed GT varied more according to their geographic origin than by their trap hosts of isolation. In other cases, there were no apparent relationships with either the legume or geographic source. The MLST method that was developed should be a useful tool in determining the influence of geographic location, temperature, season, soil type, and host plant cultivar on the distribution of GT of Bradyrhizobium spp.

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“…In addition, there are 4 recognized strains of slow-growing soybean rhizobia, i.e., Bradyrhizobium elkanii, B. japonicum, B. liaoningense, and the recently-discovered B. yuanmingense biovar that nodulates soybeans [6][7][8][9] . At present, there is not much information on polyphasic taxonomy of soybean rhizobia in Thailand [10][11][12][13][14][15] . Strains of soybean rhizobia in Thailand could be used as a pool to select soybean rhizobia suitable for the production of biofertilizers.…”

Section: Introductionmentioning

confidence: 99%

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Maruekarajtinpleng¹,

Homhaul²,

Chansa-ngavej³

2012

ScienceAsia

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Soybean rhizobia are Gram negative bacteria that fix nitrogen in root nodules of soybeans. Selection of soybean rhizobia from present and previous soybean cultivation areas is one way to obtain efficient strains for inoculant production. At present, information on the diversity of soybean rhizobia in Thailand is scarce. The experiments aimed to isolate and characterize soybean rhizobium strains in soils from 16 subdistricts in Phitsanulok province, Thailand. Host trapping method was used to isolate bacteria from root nodules of 5 soybean cultivars grown in soils from the 16 subdistricts. Identical RAPD-PCR fingerprints revealed the 202 slow-growing isolates consisted of 121 strains. Authentication tests using 5 soybean cultivars showed all the 121 slow-growing strains had nodulated soybean roots. Four types of colony morphology on yeast extract mannitol containing congo red agar plates were obtained for all the isolated strains. Bromothymol blue (BTB) reactions on BTB agar plates revealed two types of slow-growing soybean rhizobia. Type 1 secreted alkali products after 5-day incubation, and acidic products upon prolonged incubation for another 5 days. Type 2 secreted only alkali products after 5-and 10-day incubation. Results from nucleotide sequences of 16S rDNA revealed 7, 6, and 2 strains of Bradyrhizobium elkanii, B. japonicum, and B. yuanmingense, respectively. The B. elkanii and B. japonicum strains were found to be natural variants with different RAPD-PCR fingerprints. This study is the first report on the findings of B. yuanmingense as well as natural variants of slow-growing soybean rhizobia in Thailand.

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Serological properties and intrinsic antibiotic resistance of soybean bradyrhizobia isolated from Thailand

Cited by 14 publications

References 33 publications

Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov.

Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum group Ia strains as Bradyrhizobium diazoefficiens sp. nov.

Development and Application of a Multilocus Sequence Analysis Method for the Identification of Genotypes Within Genus Bradyrhizobium and for Establishing Nodule Occupancy of Soybean (Glycine max L. Merr)

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