Serological Proteomics of Gastritis: Degradation of Apolipoprotein A-I and Alpha1-Antitrypsin Is a Common Response to Inflammation Irrespective of Helicobacter pylori Infection

He, Qing-Yu; Huai-yi, Yang; Wong, Benjamin Chun–Yu; Chiu, Jen‐Fu

doi:10.1007/s10620-008-0269-z

Cited by 7 publications

(2 citation statements)

References 48 publications

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“…It has long been known that both proteins APOD and APOH play essential roles in regulating lipoproteins and act as cofactors of lipoprotein lipase . Similarly, He et al found that APOAI and A1AT were degraded during inflammation, suggesting that they are both acute-phase proteins.…”

Section: Resultsmentioning

confidence: 99%

Site-Specific Glycosylation Quantitation of 50 Serum Glycoproteins Enhanced by Predictive Glycopeptidomics for Improved Disease Biomarker Discovery

Kailemia

Merleev

et al. 2019

Anal. Chem.

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Analysis of serum protein glycovariants has the potential to identify new biomarkers of human disease. However, the inability to rapidly quantify glycans in a site-specific fashion remains the major barrier to applying such biomarkers clinically. Advancements in sample preparation and glycopeptide quantification are thus needed to better bridge glycoscience with biomarker discovery research. We present here the successful utilization of several sample preparation techniques, including multienzyme digestion and glycopeptide enrichment, to increase the repertoire of glycopeptides that can be generated from serum glycoproteins. These techniques combined with glycopeptide retention time prediction and UHPLC-QqQ conditions optimization were then used to develop a dynamic multiple-reaction monitoring (dMRM)-based strategy to simultaneously monitor over 100 glycosylation sites across 50 serum glycoproteins. In total, the abundances of over 600 glycopeptides were simultaneously monitored, some of which were identified by utilizing theoretically predicted ion products and presumed m/z values. The dMRM method was found to have good sensitivity. In the targeted dMRM mode, the limit of quantitation (LOQ) of nine standard glycoproteins reached femtomole levels with dynamic ranges spanning 3–4 orders of magnitude. The dMRM-based strategy also showed high reproducibility with regards to both instrument and sample preparation performance. The high coverage of the serum glycoproteins that can be quantitated to the glycopeptide level makes this method especially suitable for the biomarker discovery from large sample sets. We predict that, in the near future, biomarkers, such as these, will be deployed clinically, especially in the fields of cancer and autoimmunity.

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Section: Resultsmentioning

confidence: 99%

Site-Specific Glycosylation Quantitation of 50 Serum Glycoproteins Enhanced by Predictive Glycopeptidomics for Improved Disease Biomarker Discovery

Kailemia

Merleev

et al. 2019

Anal. Chem.

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“…Відомо, що інгібітори протеїназ мають також протизапальну, антибактеріальну, противірусну і протипухлинну дії [12]. Виявлено велику кількість варіантів реагування інгібіторів протеолізу: від підвищення їх активності [11], до зниження [10] і різноспрямованої реакції [7]. Неоднозначність оцінки ролі інгібіторів протеолізу в реакціях адаптації та розвитку патологічних процесів призводить до істотних труднощів при використанні їх як діагностичних і прогностичних критеріїв.…”

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Plasma proteolytic and fibrinolytic activity in non-alcoholic steatohepatitis, combined with chronic obstructive pulmonary disease

Tsyntar¹

2014

Buk. Med. Herald

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The study involved 30 patients with NASH, 30 patients with chronic obstructive pulmonary disease and 30 patients with non-alcoholic steatohepatitis in combination with chronic obstructive pulmonary disease. A substantial enhancement of proteolysis low and high molecular weight proteins, of colagenolytic activity of plasma and nonenzymatic fibrinolysis against reduction of content of α2-macroglobulin in serum and more pronounced decrease in activity of the enzymatic fibrinolysis in this combined pathology.

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Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

et al. 2010

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Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life-threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2-DE and Western blot methods were employed to study the parasite circulating antigens and host-specific proteins in the sera of T. gondii-infected individuals. The comparisons were made between serum protein profiles of infected (n=31) and normal (n=10) subjects. Antigenic proteins were identified by immunoblotting using pooled sera and monoclonal anti-human IgM-HRP. Selected protein spots were characterised using mass spectrometry. Prominent differences were observed when serum samples of T. gondii-infected individuals and normal controls were compared. A significant up-regulation of host-specific proteins, α(2)-HS glycoprotein and α(1)-B glycoprotein, was also observed in the silver-stained gels of both active and chronic infections. However, only α(2)-HS glycoprotein and α(1)-B glycoprotein in the active infection showed immunoreactivity in Western blots. In addition, three spots of T. gondii proteins were detected, namely (i) hypothetical protein chrXII: 3984434-3 TGME 49, (ii) dual specificity protein phosphatase, catalytic domain TGME 49 and (iii) NADPH-cytochrome p450 reductase TGME 49. Thus, 2-DE approach followed by Western blotting has enabled the identification of five potential infection markers for the diagnosis of toxoplasmosis: three are parasite-specific proteins and two are host-specific proteins.

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Serological Proteomics of Gastritis: Degradation of Apolipoprotein A-I and Alpha1-Antitrypsin Is a Common Response to Inflammation Irrespective of Helicobacter pylori Infection

Cited by 7 publications

References 48 publications

Site-Specific Glycosylation Quantitation of 50 Serum Glycoproteins Enhanced by Predictive Glycopeptidomics for Improved Disease Biomarker Discovery

Site-Specific Glycosylation Quantitation of 50 Serum Glycoproteins Enhanced by Predictive Glycopeptidomics for Improved Disease Biomarker Discovery

Plasma proteolytic and fibrinolytic activity in non-alcoholic steatohepatitis, combined with chronic obstructive pulmonary disease

Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

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