Serological reactivity to heat shock protein 70 in patients with hydatid disease

Abstract

Heat shock protein 70 (hsp70) was chosen as a model antigen with which to investigate autoantibody production in humans with cystic hydatid disease. Levels of specific serum antibody were assessed in the sera of patients with surgically confirmed infection with E. granulosus and sera from non-infected controls. Antigens used were human hsp70, obtained from K562 cells grown in culture, and E. granulosus hsp70 obtained by expression of the full length protein in Escherichia coli following cloning of the associated mRNA. Antibody reactivity to human hsp70 was detected in the sera of only a small proportion of hydatid patients (10%) as well as a similar proportion of sera from age matched controls. Specific antibodies reactive with E. granulosus hsp70 were detected in 60% of hydatid patients, although some samples (21%) from healthy controls also reacted with E. granulosus hsp70, the level of reactivity was significantly higher in hydatid patients. This report identifies E. granulosus hsp70 as an immunogen during human hydatid infection but, despite its having a predicted 81% protein sequence homology with human hsp70, it does not appear to induce autoimmune reactivity against the homologous human protein.

Recognition of a Dominant Epitope in Bovine Heat‐Shock Protein 70 in Inner Ear Disease

“…Infection, especially chronic infection, might therefore be expected to induce antibodies to both the HSP70 of the infectious agent and to self‐HSP70. Nevertheless, with the exception of Plasmodium falciparum malaria, in which antibodies to human HSP70 were detected in 48 of 62 sera (77%) from Brazilian patients, 17 such autoantibodies are usually found in lesser frequency in infected individuals 15,18 . In the present study, sera from only 12 of 52 patients with IPBSNHL (23%) reacted with rhHSP70.…”

Anti–Heat Shock Protein 70 Antibodies in Meniere's Disease

“…Using an enzyme‐linked immunosorbent assay (ELISA) in which recombinant HSP70 of Leishmania donovani origin was used as antigen, Arora et al 49 found anti‐HSP70 antibodies in 22 of 26 (85%) of patients with SLE and 12 of 20 (60%) of control serum sample. In another ELISA‐based study, employing recombinant Echinococcus granulosus HSP70, antibodies were detected in 60% of serum samples from hydatid patients and 21% of samples from healthy controls 50 ; the level of reactivity was significantly higher in hydatid patients. In the present study, we were unable to distinguish reactive control from reactive patient serum by serial dilution (data not shown).…”

Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis

“…1993, Ersfeld & Craig 1995, Martin et al . 1996, Colebrook & Lightowlers 1997). We have recently isolated an elongation factor‐1β/δ from a cDNA library of E. granulosus and have investigated its role in the host–parasite relationship.…”