Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy

Cho, Sang Nae; Yanagihara, Donna L.; Hunter, S W; Gelber, Robert H.; Brennan, Patrick J.

doi:10.1128/iai.41.3.1077-1083.1983

Cited by 206 publications

(76 citation statements)

References 15 publications

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“…GPLs from isolates and standards were suspended in absolute ethanol (1 mg ml −1 ) by sonication in a Bransonic ultrasonic cleaner 1200 (Branson, Shelton, CT, USA). Aliquots of 100 ml were applied to round-bottommed wells of 96-well polystyrene microtitre plates (Immulon 2, Dynatech Laboratories, Inc., Chantilly, VA, USA) as described previously (Cho et al 1983) and were dried overnight at room temperature. Then, plates were washed with phosphate-buffered saline (PBS) containing 0·05% (v/v) Tween-20, and were blocked with 200 ml well −1 10% goat serum (Gibco BRL, Life Technologies, Grand Island, NY, USA) in PBS containing 0·05% (v/v) Tween-20.…”

Section: Elisamentioning

confidence: 99%

“…As primary antibody, rabbit antiserum to the known serovars (Schaefer 1980;Tsang et al 1983), diluted in PBS-Tween, was added. The ELISA was completed as described previously (Cho et al 1983). For some clinical isolates, ELISA was based on specific murine Mabs for individual GPLs, to allow accurate identification and avoid crossreactivities inherent in the polyclonal antibodies (Rivoire et al 1989).…”

Section: Elisamentioning

confidence: 99%

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Serovars of Mycobacterium avium complex isolated from AIDS and non-AIDS patients in Spain

Torrelles¹,

Chatterjee²,

Lonca³

et al. 2000

J Appl Microbiol

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NA N . 2000. Antigen fingerprinting based on surface glycolipid antigens was applied to the epidemiology of clinical isolates of the Mycobacterium avium complex from 128 acquired immunodeficiency syndrome (AIDS) and 31 non-AIDS patients from several different regions of Spain. The application of thin-layer chromatography, gas chromatography-mass spectrometry and monoclonal antibodies, combined with ELISA, allowed a facile identification, differentiation and classification of the isolates. The cumulative results demonstrate that, among the clinical isolates, serovar 4 was predominant in both AIDS (33·6%) and non-AIDS (22·6%) isolates. In general, the results demonstrate geographical as well as disease-related differences in the distribution of Myco. avium complex serovars of clinical importance.

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Section: Elisamentioning

confidence: 99%

Section: Elisamentioning

confidence: 99%

Serovars of Mycobacterium avium complex isolated from AIDS and non-AIDS patients in Spain

Torrelles¹,

Chatterjee²,

Lonca³

et al. 2000

J Appl Microbiol

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“…In the traditional PGL-1ELISA method, di-saccharide-bovine serum albumin (D-BSA) and bovine serum albumin (BSA) are used as antigen and control respectively while phosphate buffer saline is used as buffer [10]. The ELISA test was based on a synthetic conjugate containing the carbohydrate epitope of PGL-1 [11]; the major antigenic glycolipid in the bacterium. The principal antigenic determinant of PGL-1 is the di-and trisaccharide of the molecule [12].…”

Section: Introductionmentioning

confidence: 99%

Epidemiological Surveillance of Leprosy in Cameroon Using the ELISA Test with D-BSA and Tris-HCl as Buffer

Nsagha¹,

Bamgboye²,

Ls³

et al. 2014

AJEID

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Background: Early detection of leprosy is important for elimination, but most cases are diagnosed only when lesions occur and nerves and skin are damaged. The determination of the risk of contacts to develop leprosy is still unpredictable, and elimination is not possible as long as contacts can still develop the disease. Methods: A modified PGL-I ELISA using tris-HCl as buffer and D-BSA as antigen evaluated IgM leprosy antibodies for the detection of subclinical leprosy infection and monitor effective chemotherapy. This case-control study took place in a leprosy endemic area among lepers diagnosed clinically and bacteriologically, intra-familial and extra-familial contacts and controls consisting of patients attending a health facility for reasons other than leprosy. Results: The highest mean absorbance of positive sera was from leprosy patients (0.14 ± 0.06), contacts (0.12 ± 0.05) and controls (0.04±0.04). The proportion of sero-positive subjects for IgM anti-bodies to M. leprae was 43.5 % (95% CI: 37.6% -49.4%). Twenty-three (44.2%) discharged cases since 1985 were still sero-positive for leprosy IgM anti-bodies; 15 years after treatment. We found a risk factor of 6 among contacts and controls suggesting a high level of transmission. Boyo division had a higher seropositivity (51.5%) compared to Mezam division (30%). Positive cases were clustered among students (44.8%), farmers (42.5%), the self-employed (44.7%), those with paid employment (37.5%) and the unemployed (60.0%) (P=0.01). Conclusion: The PGL-I ELISA with D-BSA and tris-HCl as buffer is a useful epidemiological surveillance tool for the detection of sub-clinical leprosy infection and chemotherapy monitoring. The discharged patients who tested positive should be re-evaluated for relapses.

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“…One of the principal goals of leprosy research is to develop tests that will allow early detection and timely treatment of individuals at greater risk of developing the disease. Phenolic glycolipid (PGL-I) of Mycobacterium leprae has a unique trisaccharide component and is found in large amounts in tissues of infected humans and armadillos (1). The terminal sugar of the trisaccharide makes the molecules specific and strongly antigenic, as evidenced by the antibody response to it by patients with multibacillary leprosy (2,3).…”

Section: Introductionmentioning

confidence: 99%

High Incidence of IgG Antibodies to Phenolic Glycolipid in Non‐Leprosy Patients in India

Kumar

Sinha

Sehgal

1998

The Journal of Dermatology

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Purified phenolic glycolipid (PGL-1) from Mycobacterium leprae was used to detect IgG antibodies against PGL-1 in leprosy patients in an enzyme-linked immunosorbent assay (ELISA). A total of 698 sera were screened; they came from patients suffering from leprosy, autoimmune disease, myeloma, tuberculosis and sexually transmitted diseases (STDs). Cases with miscellaneous diseases and persons undergoing AIDS screening were also included. Sera from lepromatous and tuberculoid leprosy patients gave positivity rates of 60.5% and 41.7%, respectively. In non-leprosy cases, the PGL-1 ELISA showed an overall positivity rate of 6.9%; this was greatest in patients with tuberculosis (43.8%) followed by autoimmune diseases (40.9%) and miscellaneous cases including liver diseases (37.9%). This study emphasizes that PGL-1 ELISA has a low predictive value for diagnosis of active infection by Mycobacterium leprae. Positive reactions in a significant percentage of patients with autoimmune disease are intriguing and need indepth study.

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Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy

Cited by 206 publications

References 15 publications

Serovars of Mycobacterium avium complex isolated from AIDS and non-AIDS patients in Spain

Serovars of Mycobacterium avium complex isolated from AIDS and non-AIDS patients in Spain

Epidemiological Surveillance of Leprosy in Cameroon Using the ELISA Test with D-BSA and Tris-HCl as Buffer

High Incidence of IgG Antibodies to Phenolic Glycolipid in Non‐Leprosy Patients in India

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