Serovars of Mycobacterium avium complex isolated from AIDS and non-AIDS patients in Spain

Abstract

NA N . 2000. Antigen fingerprinting based on surface glycolipid antigens was applied to the epidemiology of clinical isolates of the Mycobacterium avium complex from 128 acquired immunodeficiency syndrome (AIDS) and 31 non-AIDS patients from several different regions of Spain. The application of thin-layer chromatography, gas chromatography-mass spectrometry and monoclonal antibodies, combined with ELISA, allowed a facile identification, differentiation and classification of the isolates. The cumulative results demonstrate that, among the clinical isolates, serovar 4 was predominant in both AIDS (33·6%) and non-AIDS (22·6%) isolates. In general, the results demonstrate geographical as well as disease-related differences in the distribution of Myco. avium complex serovars of clinical importance.

Characterization of Clinical and Environmental Mycobacterium avium Spp. Isolates and Their Interaction with Human Macrophages

“…We next determined the specific serovar for each isolate by directly analyzing the GPL that defines the serovar by TLC. The specific serovar assignment for each isolate was performed based on their R f values and previous literature [26]. The results showed that all isolates, except 2, contain nsGPLs and ssGPLs (Fig.…”

“…2). Based on our previous reports [25], [26] and the limitations that TLC analysis presents [26], a tentative determination of the serovar for each isolate studied was performed. The results indicated that isolate 1 could be a mixture of Ser.…”

“…GPLs were obtained as we previously described [26]. Briefly, bacilli were scraped from agar plates and transferred directly to sterile screw-capped glass culture tubes and suspended in chloroform:methanol (2∶1, v/v) at a cell:volume ratio of approximately 1∶10.…”

“…Total GPL extractions (100 µg) were converted to alditol acetates using scyllo -inositol as an internal standard and analyzed by gas chromatography/mass spectrometry (GC/MS) as we previously described [26]. GC of alditol acetates was performed on a ThermoQuest Trace Gas Chromatograph 2000 connected to a GCQ/Polaris MS detector (ThermoQuest, Austin, TX) at an initial temperature of 50°C for 1 min, increasing to 170°C at 30°C/min and finally to 270°C at 5°C/min.…”

Serotype-Specific Modulation of Human Monocyte Functions by Glycopeptidolipid (GPL) Isolated from Mycobacterium avium Complex

“…[5][6][7][8][9][10] Among more than 31 serotypes of MAC reported so far, 11) MAC with serovar 1, 4, 8 and 9 were frequently isolated in AIDS patients, and the cumulative results demonstrated that the serovar 4 was predominant in clinical isolates of AIDS patients. 1,12) Stimulation of human peripheral blood mononuclear cells (PBMC) and MonoMAC 6 cells, a human macrophage cell line, with GPL serovar 4 induced production of cytokines TNF-a, IL-1b and IL-6 as well as lipid mediators prostaglandin E 2 and thromboxane B 2 . 13,14) When human PBMC were exposed for 24 h to GPL, especially GPL serovar 4, prior to treatment with phytohemagglutinin and phorbol myristate acetate, Th1 cytokine production such as IL-2 and IFN-g was significantly suppressed.…”