Serology and plasmid carriage in Vibrio cholerae

Newland, John W.; Voll, Mary Jane; McNicol, L A

doi:10.1139/m84-180

Cited by 15 publications

(16 citation statements)

References 25 publications

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“…Additionally, 46/187 clinical and environmental non-O1 strains were reported to carry plasmids of low molecular weight [9]. Our study provides further evidence that cryptic plasmids of low molecular weight are frequently found in V. cholerae non-O1 strains.…”

Section: Resultssupporting

confidence: 62%

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Molecular characterization and antibiotic susceptibility ofVibrio choleraenon-O1

Dalsgaard¹,

Serichantalergs

Pitarangsi

et al. 1995

Epidemiol. Infect.

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SUMMARYA collection of 64 clinical and environmental Vibrio cholerae non-01 strains isolated in Asia and Peru were characterized by molecular methods and antibiotic susceptibility testing. All strains were resistant to at least 1 and 80% were resistant to two or more antibiotics. Several strains showed multiple antibiotic resistance ( > three antibiotics). Plasmids most often of low molecular weight were found in 21/64 (33%) strains. The presence of plasmids did not correlate with antibiotic resistance or influence ribotype patterns. In colony hybridization studies 63/64 (98%) V. cholerae non-01 strains were cholera toxin negative, whereas only strains recovered from patients were heat-stable enterotoxin positive. Forty-seven Bgl I ribotypes were observed. No correlation was shown between ribotype and toxin gene status. Ribotype similarity was compared by cluster analysis and two main groups of 13 and 34 ribotypes was found. Ribotyping is apparently a useful epidemiological tool in investigations of V. cholerae non-01 infections.

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Section: Resultssupporting

confidence: 62%

“…Cryptic plasmids of low molecular weight are often found in V. cholerae non-01. These plasmids do not normally encode genes for antibiotic resistance [9].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and antibiotic susceptibility ofVibrio choleraenon-O1

Dalsgaard¹,

Serichantalergs

Pitarangsi

et al. 1995

Epidemiol. Infect.

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“…Plasmid carriage among the Vibrionaceae of medical importance appears to be sporadic, and, although high-molecular-weight, multiple-antibiotic resistance plasmids occur in V. cholerae and V. parahaemolyticus, most plasmids de- [141] nor Glassman et al [53] indicated the biotypes of their 01 serovars, but all strains were isolated during the seventh pandemic), because Cook et al (36) found that, while their El Tor strains lacked plasmids, classical strains typically contained both a 21-and a 3-megadalton plasmid. Classical strains tested were isolated from both the sixth and seventh pandemic, and HindIll restriction digests of their plasmids were identical, although no function could be assigned to these genetic elements.…”

Section: Plasmidsmentioning

confidence: 99%

“…The molecular basis for exclusion and instability of most plasmids in V. cholerae is unknown, and the apparent lack of plasmids may be complicated by difficulties encountered in isolating extrachromosomal material from vibrios (2,36,38,141). Sometimes pooling of as many as four plasmid preparations is required before bands are visible.…”

Section: Plasmidsmentioning

confidence: 99%

Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp

et al. 1988

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Recent taxonomic advances have now implicated several different Vibrio species as human pathogens. While the most common clinical presentation of Vibrio infection continues to be gastroenteritis, an increasing number of extraintestinal infections are being reported, particularly in immunocompromised individuals. Detection of Vibrio infections requires a good clinical history and the use of appropriate isolation and identification procedures by the laboratory to confirm illnesses attributed to Vibrio species. Except for Vibrio cholerae O1 and Vibrio parahaemolyticus, there is little direct evidence linking the production of a myriad of cell-associated or extracellular factors produced by each species with human disease and pathogenesis. Many questions regarding pathogenic Vibrio species remain unanswered, including their frequency and distribution in environmental specimens (water, shellfish), infective doses, virulence potential of individual isolates, and markers associated with such strains.

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“…Extracts or lysates of chromosomal and plasmid DNAs were prepared by the method of Portnoy et al (1981), Kado & Liu (1981), Newland et al (1984 and Davis et al (1980). Chromosomal and plasmid DNAs were isolated by ethidium bromide/caesium chloride density centrifugation of large scale plasmid extracts.…”

mentioning

confidence: 99%

Novel Transmissible Factors in a Non-O1 Vibrio cholerae and a Vibrio sp.

Smigocki¹,

Voll²

1986

Microbiology

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Transmissible factors encoding production of lacunae (L factors) were demonstrated in a non-O1 Vibrio cholerae and a Vibrio sp. of recent environmental origin. Lacunae were produced in lawns of non-O1 V. cholerae indicator strains under the same assay conditions as those where lacunae were produced by the well characterized P fertility plasmid of V. cholerae O1 and the V fertility factor found in a non-cholera vibrio strain. The origin of the lacunae produced by strains harbouring the V and L factors was examined. No vibriocin or phage activity was found in culture supernates or in lacunae produced by the strains, suggesting that, as in the case of the P plasmid, the lacunae probably represent sites of active mating. Unlike the P plasmid, neither the Vn or L factor could be detected or isolated by conventional plasmid techniques.

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Serology and plasmid carriage in Vibrio cholerae

Cited by 15 publications

References 25 publications

Molecular characterization and antibiotic susceptibility ofVibrio choleraenon-O1

Molecular characterization and antibiotic susceptibility ofVibrio choleraenon-O1

Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp

Novel Transmissible Factors in a Non-O1 Vibrio cholerae and a Vibrio sp.

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