Serology of potyviruses: current problems and some solutions

Shukla, D. D.; Lauricella, R.; Ward, Colin W.

doi:10.1007/978-3-7091-6920-9_6

Cited by 33 publications

(26 citation statements)

References 46 publications

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“…This crossreactivity depends on the antiserum used for the tests. Antisera produced from early bleedings are usually more specific than those from late bleedings Shukla et al, 1992).…”

Section: Serological Relationships With Other Virusesmentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

224

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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“…This crossreactivity depends on the antiserum used for the tests. Antisera produced from early bleedings are usually more specific than those from late bleedings Shukla et al, 1992).…”

Section: Serological Relationships With Other Virusesmentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

224

172

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“…Previous work on epitope analysis in the genus Potyvirus [8,36,40] has shown that virus-specific epitopes are generally located in the surface-exposed amino-terminal region, whereas those for the cross-reacting MAbs and polyclonal antisera are present in the conserved core region of the potyviral coat proteins. However, it is also known that the epitopes for the unexpected paired relationships between biologically similar and distinct potyviruses are also located in the amino-terminal region, generally consisting of the first eight amino-terminal residues [37,42].…”

Section: Discussionmentioning

confidence: 98%

“…Two procedures were developed recently to generate virus-specific MAbs to potyviruses, namely peptide-mediated electrofusion [48] and selective tolerance induction to the coat protein core region [47]. However, these procedures rely upon the availability of prior sequence information of the coat protein and facilities for peptide synthesis and are, therefore, unlikely to be used routinely in plant virus laboratories [42].…”

Section: Discussionmentioning

confidence: 99%

“…Sixty-seven amino acid residues from the amino terminus and 18 amino acid residues from the carboxy terminus of the JGMV coat protein are exposed on the virion surface, and the exposed amino-terminal region constitutes the most immunodominant region of the JGMV coat protein [36,37]. Early bleed antisera to intact JGMV particles have been shown to contain antibodies directed only to the surface-exposed amino-terminal region, whereas late bleed antisera also contain antibodies to the core region (devoid of amino and carboxy termini) of the coat protein [42]. A polyclonal antiserum produced against the dissociated core region of the JGMV coat protein was found to react with all aphid-transmitted [37] as well as mite-or whitefly-transmitted potyviruses tested [38].…”

Section: Introductionmentioning

confidence: 96%

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Characterisation and epitope analysis of monoclonal antibodies to virions of clover yellow vein and Johnsongrass mosaic potyviruses

Hewish

Xiao

Mishra

et al. 1993

Archives of Virology

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Mouse monoclonal antibodies (MAbs) against the Australian B strain of clover yellow vein (ClYVV-B) and the JG strain of Johnsongrass mosaic (JGMV) potyviruses were produced, characterised and the epitopes with which they reacted were deduced. Using intact particles of ClYVV a total of ten MAbs were obtained which reacted strongly with ClYVV-B in an enzyme-linked immunosorbent assay and Western blots. Four of these MAbs (1, 2, 4, and 13) were found to be ClYVV-specific, as they reacted with all five ClYVV strains from Australia and the U.S.A. but not with 11 strains of bean yellow mosaic (BYMV), pea mosaic (PMV), and white lupin mosaic (WLMV) viruses which, together with ClYVV, form the BYMV subgroup of potyvirses. These MAbs failed to react with eight other potyvirus species, including six which infect legumes like the viruses in the BYMV subgroup. The ClYVV MAb 10 was found to be BYMV subgroup-specific. It reacted strongly with 15 of the 16 strains of viruses in the subgroup and gave no reaction with eight other potyviruses. The other five ClYVV MAbs reacted with varying degrees of specificity with the BYMV subgroup viruses and also with other potyviruses. Eight of the ClYVV MAbs (1, 2, 4, 5, 13, 17, 21, and 22) reacted with the intact coat proteins only and not with the truncated (minus amino terminus) coat protein of ClYVV suggesting that the epitopes for these MAbs are located in the surface-exposed, amino-terminal region of the ClYVV coat protein. Comparison of published coat protein sequences of BYMV and ClYVV isolates indicated that the epitopes for the four ClYVV-specific MAbs may be in the amino-terminal region spanning amino acid residues 18 to 30, whereas those for the other four MAbs may be located in the first 17 amino-terminal amino acid residue region. The epitopes that reacted with BYMV subgroup-specific MAb 10 and MAb 30 which reacted with 20 of the 24 potyvirus isolates, are probably located in the core region of ClYVV coat protein as these MAbs reacted with the intact as well as truncated coat protein of ClYVV. Analysis, in Western blot immunoassay, of 17 MAbs raised against virions of JGMV revealed that only two MAbs (1-25 and 4-30) were JGMV-specific, whereas others displayed varying degrees of specificity to different potyviruses.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…No entanto, o isolado RIB-1 foi detectado em PTA-ELISA com o anti-soro policlonal proveniente do Texas, produzido a partir de um "pool" de isolados norte-americanos do SCMV. Esta aparente inconsistência entre os resultados dos testes biológicos e serológicos não é incomum, e a sorologia, muitas vezes, apresentase insatisfatória na identificação de membros da família Potyviridae (Shukla et al, 1992b). As relações antigênicas entre estirpes de uma espécie de Potyviridae, particularmente do gênero Potyvirus, podem ser extremamente complexas e inconsistentes, e freqüentemente relações serológicas não se correlacionam com propriedades biológicas (Koenig, 1988, Van Regenmortel, 2000.…”

Section: Fig 4 -Análise Eletroforética Dos Produtos Amplificados Porunclassified

Caracterização de um isolado do Sugarcane mosaic virus que quebra a resistência de variedades comerciais de cana-de-açúcar

et al. 2007

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Os vírus pertencentes ao subgrupo do Sugarcane mosaic virus (SCMV, gênero Potyvirus, família Potyviridae) infectam e causam mosaico em diferentes espécies botânicas da subfamília Panicoideae (família Poaceae), porém apenas o SCMV e o Sorghum mosaic virus (SrMV) infectam naturalmente cana-de-açúcar. No Brasil, a espécie SCMV parece ser o único agente causal da doença. Embora a maioria das variedades comerciais de cana-de-açúcar seja considerada resistente ou tolerante ao SCMV, relatos de incidência de mosaico em tais variedades têm ocorrido no campo. Amostras com sintomas, de diversos clones e variedades, foram coletadas em campos experimentais e comerciais de cana-de-açúcar. Dentre as variedades, a RB72-454, uma das mais plantadas no país e considerada resistente à doença, também apresentou plantas com sintomas de mosaico. As amostras foram testadas por DAS-ELISA, com anti-soros policlonais para as espécies SCMV, Johnsongrass mosaic virus (JGMV) e Maize dwarf mosaic virus (MDMV), apresentando resultados negativos. Porém, sintomas de mosaico foram observados em mudas de sorgo "Rio" e "TX2786" quando inoculadas mecanicamente com os isolados, indicando tratar-se de infecção pelo SCMV. RNA total foi extraído das folhas de cana e submetido a RT-PCR com oligonucleotídeos específicos para SCMV e SrMV. Fragmentos específicos de aproximadamente 880 pares de bases foram amplificados com os oligonucleotídeos para o SCMV, confirmando os resultados da inoculação mecânica. Os produtos de PCR foram clonados e seqüenciados. Um dos isolados de SCMV encontrado constitui uma nova estirpe, mais severa, capaz de infectar plantas da variedade RB72-454 e de outras variedades, consideradas tolerantes, no campo.

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Serology of potyviruses: current problems and some solutions

Cited by 33 publications

References 46 publications

Zucchini yellow mosaic virus

Zucchini yellow mosaic virus

Characterisation and epitope analysis of monoclonal antibodies to virions of clover yellow vein and Johnsongrass mosaic potyviruses

Caracterização de um isolado do Sugarcane mosaic virus que quebra a resistência de variedades comerciais de cana-de-açúcar

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