Seroprevalence of Echinococcus granulosus infection in a Uruguayan rural human population

Bonifacino, Rosario; Malgor, Ramiro; Barbeito, Rafael Alonso; Ballesté, Raquel; Rodríguez, Ma José Rodríguez; Botto, Carlos; Klug, Freddy

doi:10.1016/0035-9203(91)90450-d

Cited by 23 publications

(15 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean follow-up period was 4.4 years (range 3 to 5.8 years). Immunodiagnosis Anti-echinococcus antibodies were assayed in human sera using a peroxidase micro-ELISA (enzyme-linked immunosorbent assay) [4] and modified by us [2]. Circulating antigen (CAg) and immune complexes (CIC) were assayed in whole human sera using a capture antibody method [5].…”

Section: Clinical Follow-upmentioning

confidence: 99%

See 1 more Smart Citation

Albendazole treatment and serological follow-up in hydatid disease of bone

Bonifacino¹,

Dogliani²,

Craig

1997

International Orthopaedics

View full text Add to dashboard Cite

Section: Clinical Follow-upmentioning

confidence: 99%

“…Hydatid disease, or human cystic echinococcosis, is caused by Echinococcus granulosus and is a public health problem in Uruguay [12,15]; some reports indicate a prevalence higher than 1% in the human rural population [2,13,14].…”

Section: Introductionmentioning

confidence: 99%

Albendazole treatment and serological follow-up in hydatid disease of bone

Bonifacino¹,

Dogliani²,

Craig

1997

International Orthopaedics

View full text Add to dashboard Cite

“…Serologic tests for antibodies to Echinococcus in human sera. Serum samples from those individuals who demonstrated a cyst on ultrasound were tested for total immunoglobulin antibodies by micro-ELISA using crude hydatid cyst fluid antigens as previously described, 14 and for total IgG, IgG1, and IgG4 antibodies using purified antigen B from hydatid cyst fluid. 30,31 In addition, a Western blot test using crude sheep hydatid cyst fluid was used to check for specific antibody (IgG) binding to the low molecular weight (between 8 and 20 kD) hydatid cyst fluid antigen B subunits.…”

Section: Methodsmentioning

confidence: 99%

“…13 Similarly, seroprevalence studies in rural communities, using an ELISA with crude sheep hydatid cyst fluid antigen to detect antibodies (total immunoglobulin) revealed an overall seropositive rate of 1.2% (17 of 420). 14 Ovine hydatidosis has been reported in all of the 19 Departments of Uruguay with an overall prevalence rate of 34% at slaughter; a prevalence rate of 10.7% was also recorded in 5,500 dogs subjected to arecoline purgation (Orlando D, unpublished data).…”

mentioning

confidence: 99%

Human cystic echinococcosis in a Uruguayan community: a sonographic, serologic, and epidemiologic study.

Cohen¹,

Paolillo²,

Bonifacino³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. A prevalence and transmission study of human cystic echinococcosis (CE), due to infection with the dog tapeworm Echinococcus granulosus, was undertaken in the village of La Paloma in central Uruguay. The human population was registered and screened for CE by abdominal ultrasound scan as well as a number of serologic tests. Dogs were screened for E. granulosus infection by arecoline purgation as well as specific coproantigen testing. The total prevalence of human CE (new cases and those with a previous history) was 5.6% (64 of 1,149); 3.6% (40) of the cases were new ultrasound detected asymptomatic cases (mean age ϭ 45 years). Age prevalence increased from 1.1% in the 4-6-year-old group to Ͼ 11% in the Ͼ 60-year-old group; the 20-29-year-old group had a significantly higher CE rate of 7.4%, compared with younger and older age groups, and there was no difference between sexes. A CE rate of 3.9% (20 of 514) was also recorded by ultrasound for new cases in the population residing outside the village. Most of the hydatid cysts were located in the liver presenting as either univesicular cysts or a solid mass, and of those 71% and 63%, respectively, with such cyst presentations were seropositive against E. granulosus cyst fluid antigens. Two of eight individuals who were filter paper blood spot seropositive, but ultrasound scan negative, were subsequently diagnosed respectively with pulmonary hydatidosis after radiography, and hepatic hydatidosis after computed tomography scan. Of 36 households with a CE patient, 32 were single cases while four households each harbored two CE cases. This did not represent a clustered distribution within families (23 of 117). Almost 20% of the dogs from La Paloma were found infected with E. granulosus after purge examination, with a mean worm number of 67 (range ϭ 1-1,020). An additional eight dogs that were purge negative were Echinococcus coproantigen positive. The study showed that human CE is highly endemic in Uruguay, with one of the highest local prevalence rates in the world. Transmission appears to occur readily within well-developed towns, as well as on rural sheep ranches. Mass screening by ultrasound scanning with confirmatory serologic testing is an effective approach to case detection at the community level.Echinococcosis (CE) is a chronic zoonotic parasitic helminthic disease due to infection with the larval stage (hydatid) of the small dog tapeworm Echinococcus granulosus. The parasite has a global distribution but is particularly prevalent in rural areas where it is transmitted in a cycle between the dog, the definitive host, and the sheep, the intermediate host. 1 In South America, E. granulosus is endemic and a significant public health problem in several countries including Brazil, Peru, Bolivia, Chile, Argentina, and Uruguay. [2][3][4][5][6][7][8][9][10][11][12]

show abstract

“…To control the parasite, treatment of dogs with anthelmintic is the main procedure [8]. Some progress was achieved with this procedure and the prevalence in sheep was reduced significantly in some countries or areas.…”

Section: Introductionmentioning

confidence: 99%