Seroprevalence of hepatitis E virus infection in rural and urban populations, Tunisia

Houcine, Neffati; Ritter, Jacques; Feki, S.; Dron, Anne-Gaëlle; Slim, A.; Hassine, Mohsen; Hamadi, Braham; Ramière, Christophe; Pèlegrin, André; Aouni, Mahjoub; Scholtès, Caroline

doi:10.1111/j.1469-0691.2012.03793.x

Cited by 18 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study found the anti-HEV IgG seroprevalence to be higher in male than female donors in agreement with previous studies (38)(39)(40)(41). The rate also increased with age in agreement with previous findings (37,(41)(42)(43). In this way, HEV screening in blood transfusions also should be considerate for male and over 40 years old blood donors, as they appears as a risk group.…”

Section: Discussionsupporting

confidence: 93%

Prevalence of Hepatitis E Virus Antibodies Among Blood Donors: A Systematic Review and Meta-Analysis

et al. 2017

View full text Add to dashboard Cite

Context: Hepatitis E virus (HEV) is well-known to be transmitted by the fecal-oral route. In addition, several studies in Europe and Asia had reported potential HEV transmission associated with blood transfusion, but this route is still uncertain.Objective: The aim of this study was to estimate the anti-HEV IgG seroprevalence among blood donors around the world using the Bayesian-based methods.Evidence Acquisition: A systematic review was performed using the PubMed/Medline, Scopus, Web of Science and Cochrane Library databases using the terms "prevalence", "hepatitis E" and "blood donors". Studies with a timeframe from inception to March 2016, in Roman characters, that had outcomes of interest such as prevalence of IgG antibodies were included. The estimation of anti-HEV IgG (presented as the event rate and 95% confidence intervals, CI) was performed using a Bayesian-based random effect model using the Comprehensive Meta-Analysis software, version 2. Pairwise meta-analyses and chi-square tests were used to assess significant differences between different sexes and ages. Results and Conclusions:Of the retrieved studies, 71 met the inclusion criteria, comprising a total of 113 316 blood donors. The prevalence rate was statistically significant in males and over 40 years old donors (P < 0.05). The overall estimation of the anti-HEV IgG prevalence was 0.058 (CI 95%: 0.049 -0.068). Subgrouping by region, the estimates were higher for Asia and the Middle East, respectively: 0.113 (CI 95%: 0.040 -0.278) and 0.112 (CI 95%: 0.081 -0.152). Europe, Africa, Oceania and America had an estimated prevalence between 0.014and 0.088. A relevant prevalence of anti-HEV IgG among blood donors was found worldwide, especially in Asia and the Middle East and in males and donors over 40 years. This review reinforces the view that HEV parenteral route infection needs to be further investigated and possibly screened for in transfused blood.

show abstract

Section: Discussionsupporting

confidence: 93%

Prevalence of Hepatitis E Virus Antibodies Among Blood Donors: A Systematic Review and Meta-Analysis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In Northern Tunisian regions, by using the Globe Diagnostics assay, reported seroprevalences were 12;1% among pregnant women [7], 28.9% among polytransfused patients [7], 10.2% among hemodialysis patients [6] and 4,5% among blood donors [6]. A similar low prevalence of 5.4% was reported among blood donors by using the Genelabs Diagnostics assay [8]. By using a more sensitive assay, our study thus suggests that Southern Tunisia is indeed a low endemicity region for HEV.…”

Section: Discussionmentioning

confidence: 67%

Southern Tunisia: A still high endemicity area for hepatitis A

et al. 2017

View full text Add to dashboard Cite

BackgroundHepatitis A (HAV) and E (HEV) viruses are responsible for enterically transmitted hepatitis. Tunisia is reported to be of intermediate endemicity for HAV and of low seroprevalence for HEV; however, data from rural areas of South Tunisia are lacking.MethodsSera from 216 asymptomatic pregnant women and from 92 patients with acute hepatitis were collected between October 2014 and November 2015. Total and IgM anti-HAV immunoglobulins and anti-HEV IgG and IgM were investigated. Anti-HAV IgM-positive samples were subjected to RT-PCR targeting the VP1/2A region and sequenced. HEV IgM positive samples and all samples from acute hepatitis patients were assessed for HEV RNA.ResultsAmong pregnant women (mean age 32+/-8), HAV seroprevalence was 98.6%, none presented anti-HAV IgM; HEV seroprevalence was 5.1% and three presented weakly reactive anti-HEV IgM without detectable RNA. Among acute hepatitis patients (mean age 18.5 +/- 14), HEV seroprevalence was 19,5%, none presented anti-HEV IgM, nor HEV RNA. HAV seroprevalence exceeded 90% by age 5 and acute HAV infection was detected in 20 patients (21,7%), younger than patients with other hepatitis causes (9,8 years vs. 20,4 years, p = 0,004); 65% were male. Most acute HAV infections were observed in a coastal area where HAV infections represented 52% of hepatitis etiology. Phylogenetic analysis identified genotype IA strains, clustering close to previously published Tunisian sequences.ConclusionThe present study confirmed a low HEV endemicity and evidenced a still high level of HAV circulation in Southern Tunisia, suggesting distinct dissemination patterns for these viruses.

show abstract

“…Similarly, previous studies revealed higher rates of HEV infection in individuals with a low education level (Bortoliero et al 2006, Martins et al 2014). Additionally, other studies have reported that low educational level was statistically associated with HEV seropositivity (Junaid et al 2014, Yoon et al 2014) as well as living in rural area (Mansuy et al 2011, Houcine et al 2012). Most people who live in rural areas are farmers, suggesting potential exposure to HEV, either by contact with animals or by poor sanitary conditions (Labrique et al 2009, Goumba et al 2011, da Silva et al 2012, Junaid et al 2014).…”

Section: Discussionmentioning

confidence: 94%

Hepatitis E virus infection in patients with acute non-A, non-B, non-C hepatitis in Central Brazil

Freitas

Santana

Silva

et al. 2016

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Hepatitis E virus (HEV) infection has a worldwide distribution and represents an important cause of acute hepatitis. This study aims to investigate the occurrence of HEV infection and factors associated with this infection in patients with acute non-A, non-B, non-C hepatitis in Central Brazil. From April 2012 to October 2014, a cross-sectional study was conducted among 379 patients with acute non-A, non-B, non-C hepatitis in the City of Goiania, Central Brazil. Serum samples of all patients were tested for serological markers of HEV infection (anti-HEV IgM and IgG) by ELISA. Positive samples were confirmed using immunoblot test. Anti-HEV IgM and IgG positive samples were tested for HEV RNA. Of the 379 serum samples, one (0.3%) and 20 (5.3%) were positive for anti-HEV IgM and IgG, respectively. HEV RNA was not found in any sample positive for IgM and/or IgG anti-HEV. After multivariate analysis, low education level was independently associated with HEV seropositivity (p = 0.005), as well as living in rural area, with a borderline p-value (p = 0.056). In conclusion, HEV may be responsible for sporadic self-limited cases of acute hepatitis in Central Brazil.

show abstract

Seroprevalence of hepatitis E virus infection in rural and urban populations, Tunisia

Cited by 18 publications

References 13 publications

Prevalence of Hepatitis E Virus Antibodies Among Blood Donors: A Systematic Review and Meta-Analysis

Prevalence of Hepatitis E Virus Antibodies Among Blood Donors: A Systematic Review and Meta-Analysis

Southern Tunisia: A still high endemicity area for hepatitis A

Hepatitis E virus infection in patients with acute non-A, non-B, non-C hepatitis in Central Brazil

Contact Info

Product

Resources

About