Seroprevalence of<i>Neospora caninum</i>infection following an abortion outbreak in a dairy cattle herd

Atkinson, R; Cook, R W; Reddacliff, L.A.; Rothwell, J.; Broady, Kevin W.; Harper, P. A. W.; Ellis, John

doi:10.1111/j.1751-0813.2000.tb11752.x

Cited by 58 publications

(40 citation statements)

References 34 publications

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“…In conclusion, this report describes the high seroprevalence of N. caninum infection in dairy cows in Thailand and other countries such as Brazil (14.1%, Gondim et al 1999), Australia (24.0%, Atkinson et al 2000), USA (16.0%, Rodriguez et al 2002) and Vietnam (5.5%, Huong et al 1998). Further serological surveys in dogs, lived in the surrounding area of infected farm need to be accomplished and also seroprevalence study in other regions, as well as, the association between seropositive status and bovine abortions in this country.…”

Section: Discussionmentioning

confidence: 99%

“…The seroprevalence of N. caninum in cattle or dairy cows had been studied in many countries (Gondim et al 1999;Atkinson et al 2000;Bae et al 2000;Huong et al 1998;Jenkins et al 2000;Ooi et al 2000). The prevalences were varied among countries, regions, herds and even at different times of the same herds (Yu et al 2007), but there were a few reports on bovine neosporosis in Southeast Asia (Huong et al 1998;Suteeraparp et al 1999;Kashiwasaki et al 2001;Jittapalapong et al 2008;Arunvipas et al 2012).…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Inpankaew¹,

Jittapalapong

Mitchell

et al. 2014

Acta Parasitologica

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Neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of neosporosis, recognized as a major cause of bovine abortion around the world. Thailand is a developing agricultural country located in Southeast Asia. Livestock developments particularly in dairy cows of this country have been hampered by low productivity including milk and slow growth rate due to the impact of many pathogens including N. caninum. Currently, there is no effective method for control of neosporosis since there is less information regarding current status of infections. The objective of this study was to investigate the seroprevalence of neosporosis in dairy cows of the northern part of Thailand. During 2006-2007, the sera of 642 cows from 42 small farm holders with the top three highest consensus of dairy farms in the northern provinces, such as Chiang Rai, Chiang Mai and Lumpang were collected and performed tests. Antibodies to N. caninum were assayed by enzyme-linked immunosorbent assay (ELISA) with recombinant N. caninum surface antigen 1 (NcSAG1) and indirect fluorescent antibody test (IFAT). The overall prevalence of N. caninum infection in this study was 46.9% (301/642) by ELISA and 34.3% (220/642) by IFAT.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Inpankaew¹,

Jittapalapong

Mitchell

et al. 2014

Acta Parasitologica

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“…Em bovinos, diversos estudos demonstraram que a via de transmissão vertical ou congênita do N. caninum é considerada a mais importante e pode ocorrer por diversas gerações (BARR et al, 1993;BJÖRKMAN et al, 1996BJÖRKMAN et al, , 2003HIETALA, 1997a;WOUDA et al, , 1999aDAVISON et al, 1999b,c;ATKINSON et al, 2000;BERGERON et al, 2000;DIJKSTRA et al, 2001aDIJKSTRA et al, , 2003LÓPEZ-GATIUS et al, 2004a;HALL et al, 2005;FRÖSLING et al, 2005;CHANLUN et al, 2007;, WOODBINE et al, 2008.…”

Section: Transmissão Verticalunclassified

“…Entretanto, taxa de transmissão vertical menor que 73% foram relatadas em estudos transversais, que não usaram amostras de sangue pré-colostrais (MAINAR-JAIME et al 1999;DYER et al, 2000;BERGERON et al, 2000;ATKINSON et al, 2000;DIJKSTRA et al, 2003;FRANKENA, 2003;PAN et al, 2004;BARTELS et al, 2007;CHANLUN et al, 2007;DIJKSTRA et al, 2008;MORÉ et al, 2009MORÉ et al, , 2010. A mais baixa taxa de transmissão vertical nestes estudos pode ser resultado da ocorrência de transmissão horizontal na vaca ou de seu bezerro após o parto ou declínio do título de anticorpos contra N. caninum abaixo dos níveis de detecção do teste empregado na data do sorodiagnóstico.…”

Section: Transmissão Verticalunclassified

“…Diversos estudos demonstraram que vacas soropositivas para N. caninum foram mais prováveis de ter histórico de abortamento que vacas soronegativas MAINAR-JAIME et al, 1999;DAVISON et al, 1999c;ATKINSON et al, 2000;PEREIRA BUENO et al, 2000;THOMPSON et al, 2001;CORBELLINI et al, 2002CORBELLINI et al, , 2006aHERNANDEZ et al, 2002;MOORE et al, 2003MOORE et al, , 2009VÁCLAVEK et al, 2003;LÓPEZ-GATIUS et al, 2004a,b, 2005aQUINN et al, 2004;HOBSON et al, 2005;GARCIA-VAZQUEZ et al, 2005;KOIWAI et al, 2005;WALDNER, 2005;BARTELS et al, 2006;STÅHL et al, 2006;OSHIRO et al, 2007;, 1999 caninum.…”

Section: Abortamentounclassified

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Aspectos epidemiológicos da infecção por Neospora caninum em bovinos leiteiros da região do Vale do Paraíba Paulista

Cardoso¹

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Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum

et al. 2013

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Neosporosis is a disease caused by the apicomplexan parasite Neospora caninum, which is closely related to Toxoplasma gondii. N. caninum infection represents an important cause of reproductive failure in sheep, goats, horses, and cattle worldwide. The diagnosis of neosporosis is based on the detection of pathogen-specific antibodies in animal sera or the presence of tissue cysts. However, morphological similarities and serological cross-reactivity between N. caninum and T. gondii can result in the misdiagnosis. In this study, the N. caninum tachyzoite surface protein Ncp-43 was expressed in a recombinant form to elicit polyclonal antibodies (pAb) response. The pAb was purified and conjugated to horseradish peroxidase (HRP) or fluorescein isothiocyanate (FITC) to detect the recombinant and native Ncp-43 proteins, respectively. The pAb and pAb/HRP were able to recognize rNcp-43 by dot blot and ELISA, and pAb/FITC immunolabeled the apical complex of tachyzoites. A blocking enzyme-linked immunosorbent assay (b-ELISA) was performed to evaluate pAb/HRP as a diagnostic tool. The mean percent inhibition for the positive and negative serum samples from cattle with neosporosis was significantly different (P < 0.0001). These results suggest that the pAb may bind to the same epitopes of Ncp-43 as anti-N. caninum antibodies in the positive samples tested. The b-ELISA using the pAb/HRP can facilitate diagnostic testing for neosporosis, since fewer steps are involved, and cross-reactivity with secondary antibodies is avoided. In summary, this report describes the production of antibodies against N. caninum, and evaluates the potential of these tools for the development of new diagnostic tests for neosporosis.

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Seroprevalence ofNeospora caninuminfection following an abortion outbreak in a dairy cattle herd

Cited by 58 publications

References 34 publications

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Aspectos epidemiológicos da infecção por Neospora caninum em bovinos leiteiros da região do Vale do Paraíba Paulista

Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum

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