Seroprevalence of neosporosis in beef and dairy cattle breeds in Northeast Hungary

Hornok, Sándor; Edelhofer, Renate; Hajtós, I.

doi:10.1556/avet.54.2006.4.6

Cited by 16 publications

(15 citation statements)

References 17 publications

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“…The seroprevalence (27.7%) in 3 counties (Arad, Bihor, and Timiş) was lower, however, than that previously reported in cattle with reproductive problems from dairy farms in central and northern regions of the country (55.9%; Gavrea and Cozma, 2010). Compared to several other European countries, our seroprevalence was higher than that reported in Slovakia (20.1%; Reiterová et al, 2009) and Hungary (2.5%; Hornok et al, 2006), similar to that found in the northwest of Spain (23.2%; Eiras et al, 2011) but lower than that reported in Italy (32.0%; Rinaldi et al, 2007) and Turkey (46.5%; Kul et al, 2009).…”

supporting

confidence: 75%

“…0.05) was found between the seroprevalence of N. caninum antibodies associated with the breed of the animals. Several studies have shown that dairy cattle are more susceptible to N. caninum than are beef cattle (Bartels et al, 2006;Hornok et al, 2006;Rinaldi et al 2007), and vice versa (Eiras et al 2011), but breed-related susceptibility to infection was not demonstrated; in addition, the role of farm dogs was not investigated in these studies.…”

mentioning

confidence: 95%

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Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Imre¹,

Morariu²,

Ilie³

et al. 2012

Journal of Parasitology

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Serum samples from 376 randomly selected adult cattle, from 25 farms located in 3 counties (Arad, Bihor, and Timiş) from western Romania, were sampled for Neospora caninum antibodies using a commercial ELISA-kit. Seroprevalence values and risk factors for neosporosis (cow age, breed, herd size, farming system, previous abortion, and number of farm dogs) were examined using a generalized linear mixed model with a binomial distribution. Overall, the seroprevalence of N. caninum was 27.7% (104/376) with a prevalence of 27.9% (24/86) in Arad, 26.9% (25/93) in Bihor, and 27.9% (55/197) in Timiş. Of 25 cattle herds, 23 were seropositive with a prevalence ranging from 10.0 to 52.2%. No correlation was found between N. caninum seropositivity and age, breed, herd size, breeding system, and previous abortion. The number of farm dogs was the only factor (P Wald 5 0.03) positively associated with seroprevalence in cows and can be considered the risk factor in the acquiring of infection. The present work is the first regarding serological evidence of N. caninum infection in cattle from western Romania.

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supporting

confidence: 75%

mentioning

confidence: 95%

Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Imre¹,

Morariu²,

Ilie³

et al. 2012

Journal of Parasitology

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“…ND = not done; -(negative); + (positive); JER (Jersey); HOL (Holstein); J/H (Jersey/Holstein); Legend for histopathology: 0, absent; +, discrete; ++, moderate; +++, severe; IHC = Immunohistochemistry; +1= positive in the myocardium; +2=positive in the cerebrum. (DUBEY et al, 2007;HORNOK et al, 2006). It has been demonstrated that sero-prevalence might increase with the age and the number of gestations in beef and dairy cattle (DUBEY et al, 2007;HORNOK et al, 2006), suggesting that horizontal transmission is of importance in cattle herds.…”

Section: Discussionmentioning

confidence: 99%

“…(DUBEY et al, 2007;HORNOK et al, 2006). It has been demonstrated that sero-prevalence might increase with the age and the number of gestations in beef and dairy cattle (DUBEY et al, 2007;HORNOK et al, 2006), suggesting that horizontal transmission is of importance in cattle herds.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

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The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.Keywords: Neosporosis, fetuses, lesion, serology, cattle. ResumoO objetivo deste estudo foi avaliar a ocorrência de N. caninum associado a abortamentos em vacas de leite do estado de Santa Catarina, sul do Brasil pelo uso das técnicas de ELISA (HerdCheck, IDEXX), reação em cadeia pela polimerase (PCR) e imunohistoquímica (IHC). O sangue das vacas leiteiras que abortaram, bem como, o líquido intratorácico e amostras de tecidos (cérebro, coração, fígado e pulmão) de seus fetos foram coletados e usados para sorologia, PCR (Np21+ e Np6+), e IHC. Vinte e uma vacas (51,2%) de um total de 41, bem como, oito fetos (26,7%) de um total de 30 foram positivos no ELISA (IDEXX) para N. caninum. As vacas > 36 meses de idade tiveram um maior risco de serem soropositivas do que os animais mais jovens. PCR e IHC revelaram que 38,8% e 25,0% dos fetos foram positivos para N. caninum, respectivamente para cada um dos testes. As vacas soropositivas tiveram uma maior frequência de fetos que também foram positivos no fluído intratorácico, na PCR ou na IHC. Em resumo, o presente estudo observou uma alta frequência de N. caninum em abortos de vacas leiteiras na região estudada, com maior prevalência de N. caninum em vacas com mais de 36 meses de idade. Além disso, sorologia, PCR e IHC deveriam ser utilizadas conjuntamente para melhor diagnóstico de neosporose em bovinos.

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“…This phenomenon might represent the manifestation associated with each management system, i.e., the differences between having or not access to infectious sources, the nutritional differences and emphasis of production activity (such as milking, weaning), or the variability in the duration of time during which the animals are maintained within breeding programs. However, breed predisposition for N. caninum infection cannot be excluded and should be further investigated (Hornok et al 2006). …”

Section: Discussionmentioning

confidence: 99%

Neospora caninum: evaluation of vertical transmission in slaughtered beef cows (Bos indicus)

et al. 2010

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Neospora caninum is a protozoan parasite that causes the most important reproductive problems in cattle worldwide. The objective of this study was to evaluate the possibility of vertical transmission of N. caninum in zebus breed beef cows (Bos indicus) submitted for slaughter at an abattoir in the northern region of the State of Paraná, southern Brazil. One hundred and fifty-nine cows were evaluated: 83 pregnant (in different stages of gestation) and 76 non-pregnant. Serum determination of N. caninum was evaluated by indirect ELISA (Idexx). Blood (with EDTA) from pregnant cows and tissue samples (brain and heart) from their fetuses were collected and used for PCR analyses. Antibodies against N. caninum were observed in 14.6% (12/83) of pregnant and in 15.8% (12/76) of non-pregnant cows. Antibodies against the parasites were detected in one fetus (1.4%). The PCR analyses revealed that 6.0% (5/83) of cows and 4.8% (4/83) of fetuses evaluated were positive to specific N. caninum primers. These positive fetuses were between 4 and 6 months of age. Thus, considering PCR and serology as an indicative of vertical transmission in fetuses, 4.8% of fetuses were infected by N. caninum during gestation.

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Seroprevalence of neosporosis in beef and dairy cattle breeds in Northeast Hungary

Cited by 16 publications

References 17 publications

Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Serological Survey of Neospora caninum Infection in Cattle Herds From Western Romania

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Neospora caninum: evaluation of vertical transmission in slaughtered beef cows (Bos indicus)

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