Seroprevalences for ovine enzootic abortion in Switzerland

Borel, Nicole; Doherr, Marcus G.; Vretou, Evangelia; Psarrou, Evgenia; Thoma, R.; Pospischil, Andreas

doi:10.1016/j.prevetmed.2004.08.005

Cited by 45 publications

(42 citation statements)

References 21 publications

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“…The positive results obtained by PCR and ELISA were equally distributed among the three groups and there was no significant difference in prevalence observed between the cantons of Grisons and Valais (Marreros et al, submitted; this study) as it was described previously in sheep (Borel et al, 2004). In domestic ruminants, it is well known that antibody titers are highest during lambing period and also direct antigen detection by PCR should be performed around birth time.…”

Section: Salmonella Enterica Serovar Abortusovis Chlamydophila Abortsupporting

confidence: 66%

“…Animals from Groups 1 and 2 were already used in a previous study on abortive agents (ELISA; Marreros et al, submitted), they originate from various colonies throughout the Swiss Alps. Animals from Group 3 are from a geographical region considered as a risk area for chlamydial infections according to studies performed in domestic sheep (Borel et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

“…However, seroprevalence for C. abortus is much lower in the canton of Valais (western Swiss Alps) altough management systems are similar to those in Grisons, suggesting other unknown factors favouring chlamydial abortion in small ruminants in Grisons (Borel et al, 2004). Wild ruminants (especially chamois Rupicapra r. rupicapra and Alpine ibex Capra i. ibex) graze on the same Alpine summer pastures as domestic sheep and goats.…”

Section: Introductionmentioning

confidence: 98%

“…As reported in a previous study, 39% of examined abortions in sheep and 23% in goats were caused by C. abortus (Chanton-Greutmann et al, 2002). In the canton of Grisons (eastern Swiss Alps), economic losses due to chlamydial abortion in small domestic ruminants are significantly higher than in the other cantons (Grisons: 43% seroprevalence in sheep for C. abortus, Borel et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Alpine ibex (Capra i. ibex) is not a reservoir for chlamydial infections of domestic ruminants and humans

et al. 2010

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Chlamydophila (C.) abortus is the most common infectious abortigenic agent in small domestic ruminants in Switzerland. In contrast, the knowledge about chlamydiae in wild ruminants is scarce. As interactions between livestock and Alpine ibex (Capra i. ibex) occur on alpine pastures, the question raises if wild ruminants could play a role as carriers of chlamydiae. Thus, we investigated the prevalence of chlamydiae in Alpine ibex in Switzerland. In total, 624 sera, 676 eye swabs, 84 organ samples and 51 faecal samples from 664 ibex were investigated. Serum samples were tested by two commercial ELISA kits specific for C. abortus. Eye swabs, organs and faecal samples were examined by a Chlamydiaceae-specific real-time PCR. Positive cases were further investigated by the ArrayTube (AT) microarray method for chlamydial species determination. Of 624 serum samples investigated, 612 animals were negative, whereas nine sera (1.5%) reacted positively in one of the two tests and three sera showed an inconclusive result. Eye swabs of seven out of 412 ibex (1.7%) were tested positive for Chlamydiaceae by real-time PCR. By AT microarray, Chlamydophila pecorum was identified in two animals, Chlamydophila pneumoniae was detected in one animal and a mixed infection with Chlamydophila abortus and Chlamydophila pecorum was found in four animals. Organs and faecal samples were all negative by real-time PCR analysis. In summary, we conclude that C. abortus is not a common infectious agent in the Swiss ibex population. To our knowledge, this is the first description of C. pneumoniae in ibex. Further studies are necessary to elucidate the situation in other species of wild ruminants as chamois (Rupicapra r. rupicapra), red deer (Cervus elaphus) and roe deer (Capreolus c. capreolus) in Switzerland. (C.) abortus is the most common infectious abortigenic agent in small domestic ruminants in Switzerland. In contrast, the knowledge about chlamydiae in wild ruminants is scarce. As interactions between livestock and Alpine ibex (Capra i. ibex) occur on alpine pastures, the question raises if wild ruminants could play a role as carriers of chlamydiae. Thus, we investigated the prevalence of chlamydiae in Alpine ibex in Switzerland. In total, 624 sera, 676 eye swabs, 84 organ samples and 51 faecal samples from 664 ibex were investigated. Serum samples were tested by two commercial ELISA kits specific for C. abortus. Eye swabs, organs and faecal samples were examined by a Chlamydiaceae-specific real-time PCR. Positive cases were further investigated by the ArrayTube (AT) microarray method for chlamydial species determination. Of 624 serum samples investigated, 612 animals were negative, whereas nine sera (1.5%) reacted positively in one of the two tests and three sera showed an inconclusive result. Eye swabs of seven out of 412 ibex (1.7%) were tested positive for Chlamydiaceae by real-time PCR. By AT microarray, Chlamydophila pecorum was identified in two animals, Chlamydophila pneumoniae was detected in one animal and a mixed infec...

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Section: Salmonella Enterica Serovar Abortusovis Chlamydophila Abortsupporting

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Alpine ibex (Capra i. ibex) is not a reservoir for chlamydial infections of domestic ruminants and humans

et al. 2010

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“…In Switzerland, prevalence of antibody to C. abortus in small ruminants was highest (43%) in the Canton of Grisons (Borel et al 2004) where even wild ruminants are sporadic carriers of this abortigenic agent (e.g., Holzwarth et al 2011a, b). As interactions between domestic or wild ruminants and free-living small mammals may occur on Alpine pastures, the role of the latter as reservoirs of C. abortus and other Chlamydiaceae should be considered.…”

Section: Introductionmentioning

confidence: 99%

Chlamydiaceae and Chlamydia-Like Organisms in Free-Living Small Mammals in Europe and Afghanistan

Stephan

Pospischil

Hilbe

et al. 2014

Journal of Wildlife Diseases

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ABSTRACT:Few data are available on the occurrence of chlamydial infections in wild small mammals. We investigated the significance of free-living small mammals as reservoirs or transmission hosts for microorganisms of the phylum/class Chlamydiae. We obtained 3,664 tissue samples from 911 animals in Switzerland, Germany, Austria, the Czech Republic, and Afghanistan. Samples included internal organs (n53,652) and feces (n512) from 679 rodents (order Rodentia) and 232 insectivores (order Soricomorpha) and were tested by three TaqManH real-time PCRs specific for members of the family Chlamydiaceae and selected Chlamydia-like organisms such as Parachlamydia spp. and Waddlia spp. Only one of 911 (0.11%) animals exhibited a questionable positive result by Chlamydiaceae-specific real-time PCR. Five of 911 animals were positive by specific real-time PCR for Parachlamydia spp. but could not be confirmed by quantitative PCR targeting the Parachlamydia acanthamoebae secY gene (secY qPCR). One of 746 animals (0.13%) was positive by real-time PCR for Waddlia chondrophila. This result was confirmed by Waddlia secY qPCR. This is the first detection of Chlamydia-like organisms in small wildlife in Switzerland. Considering previous negative results for Chlamydiaceae in wild ruminant species from Switzerland, these data suggest that wild small mammals are unlikely to be important carriers or transport hosts for Chamydiaceae and Chlamydia-like organisms.

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Disorders of Sheep and Goats

Moeller

2011

Kirkbride's Diagnosis of Abortion and Neonatal Loss in Animals

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Seroprevalences for ovine enzootic abortion in Switzerland

Cited by 45 publications

References 21 publications

Alpine ibex (Capra i. ibex) is not a reservoir for chlamydial infections of domestic ruminants and humans

Alpine ibex (Capra i. ibex) is not a reservoir for chlamydial infections of domestic ruminants and humans

Chlamydiaceae and Chlamydia-Like Organisms in Free-Living Small Mammals in Europe and Afghanistan

Disorders of Sheep and Goats

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