Serotonin-immunoreactive Neurons in the Antennal Sensory System of the Brain in the Carpenter Ant, Camponotus japonicus

Tsuji, Emiko; Aonuma, Hitoshi; Yokohari, Fumio; Nishikawa, Masao

doi:10.2108/zsj.24.836

Cited by 17 publications

(28 citation statements)

References 57 publications

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“…Comprehensive antennal sensillar mapping is however needed as a first step in analyzing the signal input channels of antennal sensory systems. Carpenter ants, which are common ant species and have relatively large brains, have been useful laboratory animals for electrophysiological studies (Galizia et al 1999;Ozaki et al 2005;Yamagata et al 2005Yamagata et al , 2006) and neuroanatomical studies (Gronenberg et al 1996;Ehmer and Gronenberg 2004;Kleineidam et al 2005;Tsuji et al 2007;Nishikawa et al 2008;Zube and Rossler 2008;Mysore et al 2009). Furthermore, the antennal sensilla for nestmate recognition have previously been identified by electrophysiological and biochemical studies in the ant (Ozaki et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Sex-specific antennal sensory system in the ant Camponotus japonicus: structure and distribution of sensilla on the flagellum

et al. 2009

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The antennae are a critically important component of the ant's highly elaborated chemical communication systems. However, our understanding of the organization of the sensory systems on the antennae of ants, from peripheral receptors to central and output systems, is poorly understood. Consequently, we have used scanning electron and confocal laser microscopy to create virtually complete maps of the structure, numbers of sensory neurons, and distribution patterns of all types of external sensilla on the antennal flagellum of all types of colony members of the carpenter ant Camponotus japonicus. Based on the outer cuticular structures, the sensilla have been classified into seven types: coelocapitular, coeloconic, ampullaceal, basiconic, trichoid-I, trichoid-II, and chaetic sensilla. Retrograde staining of antennal nerves has enabled us to count the number of sensory neurons housed in the different types of sensilla: three in a coelocapitular sensillum, three in a coeloconic sensillum, one in an ampullaceal sensillum, over 130 in a basiconic sensillum, 50-60 in a trichoid-I sensillum, and 8-9 in a trichoid-II sensillum. The basiconic sensilla, which are cuticular hydrocarbon-receptive in the ant, are present in workers and unmated queens but absent in males. Coelocapitular sensilla (putatively hygro- and thermoreceptive) have been newly identified in this study. Coelocapitular, coeloconic, and ampullaceal sensilla form clusters and show biased distributions on flagellar segments of antennae in all colony members. The total numbers of sensilla per flagellum are about 9000 in unmated queens, 7500 in workers, and 6000 in males. This is the first report presenting comprehensive sensillar maps of antennae in ants.

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Section: Introductionmentioning

confidence: 99%

Sex-specific antennal sensory system in the ant Camponotus japonicus: structure and distribution of sensilla on the flagellum

et al. 2009

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“…Hence, the LNs in T6 glomeruli in this study are apparently not serotonergic neurons. The existence of a specific glomerular group lacking serotonergic innervation has been defined in ants but not in honeybees (Bicker, 1999;Dacks et al, 2006;Tsuji et al, 2007;.The separate processing systems of LNs seen from our data could suggest coding differences in two parallel systems.…”

Section: Segregation Of Lns In the Primary Processing Centermentioning

confidence: 55%

“…Innervation patterns of serotonin-immunoreactive neurons in the ant brain also suggest subdivisions of the lip in mushroom body calyces (Tsuji et al, 2007;. In a hemisphere, only a single neuron innervates the basal ring and lip of the mushroom body calyces: serotonin-immunoreactive neurites innervate the medial layer of the lip, whereas the inner and outer layers lack all such neurites (Tsuji et al, 2007;Zube and Röss-ler, 2008).…”

Section: Mushroom Body Calyxmentioning

confidence: 96%

“…The distinct segregation of LNs suggests that two categories of antennal signals are processed independently in each network. The existence of two categories of glomeruli is also suggested by the patterns of innervation of serotonergic neurons in the ant antennal lobe (Dacks et al, 2006;Tsuji et al, 2007;. Carpenter ants have two types of serotonin-immunoreactive neurons, which entirely innervate non-T6 glomeruli (T1-T5 and T7) but not T6 glomeruli (Tsuji et al, 2007;.…”

Section: Segregation Of Lns In the Primary Processing Centermentioning

confidence: 99%

“…In a hemisphere, only a single neuron innervates the basal ring and lip of the mushroom body calyces: serotonin-immunoreactive neurites innervate the medial layer of the lip, whereas the inner and outer layers lack all such neurites (Tsuji et al, 2007;Zube and Röss-ler, 2008). The outer layer of the lip, which lacks serotonin-containing neurites, appears to correspond to the terminal layer of the T6 uni-PN in this study.…”

Section: Mushroom Body Calyxmentioning

confidence: 99%

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Higher brain centers for social tasks in worker ants, Camponotus japonicus

Nishikawa

Watanabe

Yokohari

2012

J of Comparative Neurology

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Ants, eusocial insects, have highly elaborate chemical communication systems using a wide variety of pheromones. In the carpenter ant, Camponotus japonicus, workers and queens have the female-specific basiconic sensilla on antennae. The antennal lobe, the primary processing center, in female carpenter ants contains about 480 glomeruli, which are divided into seven groups (T1–T7 glomeruli) based on sensory afferent tracts. The axons of sensory neurons in basiconic sensilla are thought to project to female-specific T6 glomeruli. Therefore, these sensilla and glomeruli are thought to relate to female-specific social tasks in the ants. By using dye filling into local neurons (LNs) and projection neurons (PNs) in the antennal lobe, we neuroanatomically revealed the existence of an isolated processing system for signals probably relating to social tasks in the worker ant. In the antennal lobe, two categories of glomeruli, T6 glomeruli and non-T6 glomeruli, are clearly segregated by LNs. Furthermore, axon terminals of uniglomerular PNs from the respective categories of glomeruli (T6 uni-PNs and non-T6 uni-PNs) are also segregated in the secondary olfactory centers, the calyces of the mushroom body and the lateral horn: T6 uni-PNs terminate in the outer layers of the basal ring and lip of mushroom body calyces and in the posterior region of the lateral horn, whereas non-T6 uni-PNs terminate in the middle and inner layers of the basal ring and lip and in the anterior region of the lateral horn. These findings suggest that information probably relating to social tasks might be isolated from other olfactory information and processed in a separate subsystem.

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Characterization of a multiple epigenetic marker panel for lung cancer detection and risk assessment in plasma

Hsu

Chen

Hung

et al. 2007

Cancer

135

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BACKGROUND.Methylation patterns may be useful biomarkers of cancer detection and risk assessment.METHODS.The methylation status of 6 genes, including a candidate tumor suppressor gene (BLU), the cadherin 13 gene (CDH13), the fragile histidine triad gene (FHIT), the cell cycle control gene p16, the retinoic acid receptor β gene (RARβ), and the Ras association domain family 1 gene (RASSF1A), was examined in plasma samples, corresponding tumor tissues, and normal lung tissues from a group of 63 patients with lung cancer and in plasma samples from 36 cancer‐free individuals. The detection rate of the p16 gene was validated in a test group of 20 patients with lung cancer.RESULTS.The concordance of methylation in tumor tissues and plasma samples was 86%, 87%, 80%, 75%, 76%, and 84% for the BLU, CDH13, FHIT, p16, RARβ, and RASSF1A genes, respectively. The test group showed a similar concordance for p16 methylation detection. Multiple logistic regression analysis showed that the odds ratio for having lung cancer was 10.204 for individuals with p16 methylation (P = .013) and 9.952 for individuals with RASSFIA methylation (P = .019). After several trial tests, the authors established that methylation for ≥2 of the 6 markers met the criterion for an elevated risk of cancer. Comparisons yielded a sensitivity of 73%, a specificity of 82%, and a concordance of 75% between the methylation patterns in tumor tissues and in corresponding plasma samples. The detection rate was relatively high in cigarette smokers with advanced squamous cell lung cancer.CONCLUSIONS.The current results indicated that multiple epigenetic markers in the plasma, especially the p16 and RASSF1A genes, can be used for lung cancer detection. This methylation marker panel should improve the detection of cancer or the risk assessment for lung cancer in combination with conventional diagnostic tools. Cancer 2007. © 2007 American Cancer Society.

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Serotonin-immunoreactive Neurons in the Antennal Sensory System of the Brain in the Carpenter Ant, Camponotus japonicus

Cited by 17 publications

References 57 publications

Sex-specific antennal sensory system in the ant Camponotus japonicus: structure and distribution of sensilla on the flagellum

Sex-specific antennal sensory system in the ant Camponotus japonicus: structure and distribution of sensilla on the flagellum

Higher brain centers for social tasks in worker ants, Camponotus japonicus

Characterization of a multiple epigenetic marker panel for lung cancer detection and risk assessment in plasma

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