Serotonin-induced stimulation of progesterone production by cow luteal cells in vitro

Abstract

The addition of acetylcholine or histamine (10(-7) to 10(-4) M), gamma-aminobutyric acid, a dopamine agonist, and melatonin (10(-7) to 10(-5) M) did not alter basal or LH-stimulated progesterone production (P greater than 0.05). The addition of the specific beta 2-adrenergic agonist terbutaline and salbutamol did not significantly elevate progesterone production. Treatment of luteal cells with serotonin (5-HT), 10(-6) to 10(-4) M, increased the production of progesterone (P less than 0.05). This stimulated production was inhibited by the addition of mianserin (10(-5) M, a 5-HT antagonist; P less than 0.05). Isoproterenol (10(-7) to 10(-4) M) also resulted in significant increases in progesterone production (P less than 0.05). The combined treatments of 5-HT + LH, isoproterenol + LH, or isoproterenol + 5-HT did not result in a further increase in progesterone above that observed in response to LH or isoproterenol alone (P greater than 0.05). The isoproterenol-induced progesterone production could not be blocked by butoxamine (10(-5) M, a beta 2-antagonist), or practolol (10(-5) M, a beta 1-antagonist), but was inhibited by propranolol (10(-5) M, a general beta-antagonist; P less than 0.05). The response to isoproterenol was unaffected by mianserin (10(-5) M). These results demonstrate a possible role for 5-HT in the regulation of steroidogenesis by the corpus luteum of the cow. Furthermore, these results suggest that serotonin-induced progesterone production is a receptor-mediated event.

Participation of Opioid and Serotoninergic Systems in Prolactin Secretion Induced by Hypothalamic Action of Estradiol

“…It is possible that pCPA or NAL acted directly on the ovary. Serotonin can have luteotropic actions directly on luteal cells [52,53], while opioid peptides are present in the ovary [54,55],…”

Changing responsiveness of luteal cells of the marmoset monkey (Callithrix jacchus) to luteotrophic and luteolytic agents during normal and conception cycles

“…In the rabbit, in-vitro responses to PGF-2a were dependent both on dose and time of incubation (Puri & Laumas, 1975), . In the cow, melatonin did not stimulate progesterone production in short-term incubation of luteal cells (Battista & Condon, 1986) but did stimulate progesterone production by granulosa cells in culture (Webley & Luck, 1986). This was also the case with human cells (Webley & Luck, 1986;Webley et ai, 1988).…”

Characterization of presynaptic 5‐HT receptors on adrenergic nerves supplying the bovine ovarian follicle

“…Besides the previously described motor effects on the follicle wall (O'Shea & Phillips, 1974;Gimeno et al, 1976;Talbot & Schroeder, 1982), it is suggested that 5-HT stimulates progesterone synthesis in bovine luteal cells in vitro (Battista & Condon, 1986). Also the ovarian concentration of 5-HT has been shown to increase during the oestrous period in cycling rats (Clausell & Soliman, 1978).…”