Serotonin uptake, storage, and synthesis in an immortalized committed cell line derived from mouse teratocarcinoma.

Abstract

We report the isolation and characterization of a serotoninergic cell line, iC1l, derived from a mouse teratocarcinoma. The clone iCli was immortalized through the expression of the simian virus 40 oncogenes. iCli presents two states: an immature epithelial-like state (1Cli precursor) and a more differentiated state (1C01*). After induction by

Regulation by Neurotransmitter Receptors of Serotonergic or Catecholaminergic Neuronal Cell Differentiation

“…Due to the low level constitutive expression of the SV40 T antigen, this construct promotes immortalization of neuroectodermal, endodermal, or mesodermal precursor cell lines while still allowing differentiation along multiple pathways to occur (8,9).…”

“…It maintains an undifferentiated phenotype and responds to induction by producing a progeny with neuron-associated markers. 1C11 cells convert within 4 days into serotonergic cells able to metabolize, store, and take up serotonin (5-HT) 1 (8,10) and expressing 5-HT 1B/D , 5-HT 2A , and 5-HT 2B receptors (11).…”

“…This receptor plays a pivotal role in the implementation of the catecholaminergic program, since its inhibition prevents the cells from acquiring a functional NE transport. (8). Mouse brain synaptosomes were obtained according to O'Reilly and Reith (13).…”

Prolongation of prion disease-associated symptomatic phase relates to CD3+ T cell recruitment into the CNS in murine scrapie-infected mice

“…Actually, predicting epitopes on the sole basis of their binding motif has a success rate of approximately 30% to reveal naturally processed peptides (Yewdell and Bennink, 1999). In this work, only 168 and 192 peptides were naturally processed and presented to the immune system by 1C11 cells, a neuronal cell line (Buc-Caron et al, 1990;Mouillet-Richard et al, 2000). Among the five tested NP-peptides, 168NP, 192NP and 217NP but not 103NP and 166NP induced T cells that were lytic in vivo for NP-but not native peptide-loaded target cells.…”

“…These results indicate that the immunizing peptides must be modified to ensure CTL recruitment. To determine if these peptides are naturally processed by cells expressing PrP, we used the mPrP-expressing 1C11 neuronal cell line (Buc-Caron et al, 1990;Mouillet-Richard et al, 2000), which express H-2D b class I but not I-A b class II molecules at the cell surface (data not shown). Wt mice were immunized with 168NP, 192NP, and 217NP mixed in HBV/CpG/IFA as described above.…”