Serotypes, Hemagglutinins, Siderophore Synthesis, and Serum Resistance of Klebsiella Isolates Causing Human Urinary Tract Infections

Podschun, Rainer; Sievers, D. M.; Fischer, Arno; Ullmann, U.

doi:10.1093/infdis/168.6.1415

Cited by 119 publications

(118 citation statements)

References 41 publications

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“…Isolates from affected animals at the TFZ and ZTM as well as unaffected controls were also tested for assimilation of ethanolamine, histamine, D-melezitose and DL-3-hydroxybutyrate (Sigma-Aldrich, Munich, Germany) according to Monnet and Freney (1994) Germany]) for 24 h at 378C and for an additional 24 h at room temperature to promote capsular production. The K-serotypes of the isolates were determined by the capsular swelling method using K-specific antisera as described elsewhere (Podschun et al 1993).…”

Section: Histological and Bacteriological Examinationmentioning

confidence: 99%

Klebsiella oxytoca: opportunistic infections in laboratory rodents

Bleich

Kirsch

Sahly³

et al. 2008

Lab Anim

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SummaryOpportunistic pathogens have become increasingly relevant as the causative agents of clinical disease and pathological lesions in laboratory animals. This study was conducted to evaluate the role of Klebsiella oxytoca as an opportunistic pathogen in laboratory rodents. Therefore, K. oxytoca-induced lesions were studied from 2004 to early 2006 in naturally infected rodent colonies maintained at The Jackson Laboratory (TJL), Bar Harbor, USA, the Animal Research Centre (Tierforschungszentrum, TFZ) of the University of Ulm, Germany and the Central Animal Facility (ZTM) of the Hannover Medical School, Germany. K. oxytoca infections were observed in substrains of C3H/HeJ mice, which carry the Tlr4Lps-d allele; in LEW.1AR1-iddm rats, the latter being prone to diabetes mellitus; in immunodeficient NMRI-Foxn1 nu mice; and in mole voles, Ellobius lutescens. The main lesions observed were severe suppurative otitis media, urogenital tract infections and pneumonia. Bacteriological examination revealed K. oxytoca as monocultures in all cases. Clonality analysis performed on strains isolated at the ZTM and TFZ (serotyping, pulse field gel electrophoresis [PFGE], enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction, sequencing of 16S rRNA and rpoB genes) revealed that the majority of bacteria belonged to two clones, one in each facility, expressing the capsule type K55 (ZTM) or K72 (TFZ). Two strains, one isolated at the ZTM and one at the TFZ, showed different PFGE and ERIC pattern than all other isolates and both expressed capsule type K35. In conclusion, K. oxytoca is an opportunistic pathogen capable of inducing pathological lesions in different rodent species.

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Section: Histological and Bacteriological Examinationmentioning

confidence: 99%

Klebsiella oxytoca: opportunistic infections in laboratory rodents

Bleich

Kirsch

Sahly³

et al. 2008

Lab Anim

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“…Most strains of K. pneumoniae produce two different types of fimbriae that mediate adhesion to host cells. Type 1 (or common) fimbriae cause mannose-sensitive haemagglutination (MSHA) and play an important role in urinary tract infections [9][10][11]. Type 3 fimbriae mediate mannose-resistant and Klebsiella-like agglutination of tanned erythrocytes (MR=K-HA) and are reported to correlate with catheter-associated bacteriuria caused by Providencia stuartii [12].…”

Section: Introductionmentioning

confidence: 99%

“…In K. pneumoniae, serum resistance properties are more common among isolates from clinical specimens than in faecal or environmental isolates [13]. Furthermore, Klebsiella isolates have been shown to produce highaffinity iron-chelating siderophores [11,14,15]. While the role of the catechol-type siderophore, enterobactin, in virulence is still uncertain, the contribution of the hydroxamate-type siderophore, aerobactin, to virulence has been demonstrated clearly [16].…”

Section: Introductionmentioning

confidence: 99%

Expression of putative virulence factors by clinical isolates of Klebsiella planticola

Podschun

Fischer

Ullman

2000

Journal of Medical Microbiology

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A total of 92 clinical isolates of Klebsiella planticola from man was examined with respect to the production of haemagglutinins and siderophores, serum resistance and distribution of capsular types. For comparison, a group of 207 clinical isolates of K. pneumoniae was also studied. The percentages of K. planticola strains able to express mannose-sensitive haemagglutination, indicating type 1 fimbriae (83%) and mannose-resistant and Klebsiella-like agglutination, indicating type 3 fimbriae (69%), as well as to produce the siderophores enterobactin (100%) and aerobactin (2.2%) were almost identical to those of the K. pneumoniae strains. Similarly, the proportion of serum-resistant strains (30%) was comparable to that of K. pneumoniae (25%). The capsule types most often detected in K. planticola were K14 (13%), K2 (9%) and K70 (9%). The incidence of K2, which is the predominant capsular type in K. pneumoniae, was similar in both species. These findings show that K. planticola, which is being detected with increasing frequency in clinical specimens from man, has the ability to express similar putative virulence factors to K. pneumoniae, suggesting that they may have similar pathogenicity.

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“…The siderophores enterobactin and aerobactin could also potentially be involved. While enterobactin is synthesized by nearly all isolates of K. pneumoniae, aerobactin is less frequently detected (Podschun et al, 1993). However, only K. pneumoniae isolates with capsular serotype K1 or K2 that produced aerobactin were more virulent (LD 50 <10 3 cfu), and transfer of the genes necessary for synthesis of aerobactin into an avirulent strain increased the virulence by 100-fold (Nassif and Sansonetti, 1986).…”

Section: Pathogenesismentioning

confidence: 99%