SEROTYPES OF <i>ESCHERICHIA COLI</i> ASSOCIATED WITH COLIBACILLOSIS IN NEONATAL ANIMALS

Glantz, Paul J.

doi:10.1111/j.1749-6632.1971.tb34994.x

Cited by 30 publications

(13 citation statements)

References 5 publications

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“…In an earlier study on colibacillosis in animals, Glantz (1971) determined the serotype of E. coli isolated from piglets, calves, lambs, young birds and rabbits. Of the 10 serotypes listed in Tables I and 2, only the first four appear on the lists of Glantz (1971), i.e., the VTEC serotypes currently most frequently reported. Of these, serotypes OIII:H-and 026:HII were associated with bovine colibacillosis, serotype 05:H-with ovine colibacillosis and serotype 0103:H2, with lapine colibacillosis.…”

Section: To Predict the Futurementioning

confidence: 99%

Role of non-O157 VTEC

Bettelheim

2000

Journal of Applied Microbiology

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SUMMARY Non‐O157 VTEC are typical Escherichia coli that differ only in their ability to produce verocytotoxins (VT). The transmission of VTEC is discussed in relation to the transmission of commensal E. coli. The emergence over the last few decades of a great variety of VTEC serotypes from healthy and diseased humans and animals is described. Particular attention is given to the distribution of the more important serogroups pathogenic for humans that have been described from around the world, particularly serogroups O26, O111, O128 and O103. The possible role of ruminants as reservoirs is discussed. The problems of laboratory diagnosis of non‐O157 VTEC are considered and various laboratory methods are assessed. Evidence is presented that the particular E. coli serotypes now known to be VTEC were present in humans and animals many years ago, but have acquired the ability to produce VT and probably other virulence factors. Finally, predictions are made of the possible increase in problems associated with these emerging pathogens.

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Section: To Predict the Futurementioning

confidence: 99%

Role of non-O157 VTEC

Bettelheim

2000

Journal of Applied Microbiology

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“…Serotyping was performed by standard methods at The E. coli Reference Center, The Pennsylvania State University (Wilson and Francis, 1986). E. coli strains were grown overnight in tryptic soy broth, then heated to 1008C for 2 h. Agglutinations were performed by using preselected dilutions of each of 183 O group antisera in microtitre plates and the positive reactions were confirmed by microtitre titrations against monovalent antisera (Glantz, 1971). If there was no agglutination in the preliminary screening assay, the bacterial suspension was reheated at 1218C for an additional 1 h and the microtitre agglutination assays were repeated.…”

Section: Serotypingmentioning

confidence: 99%

Characterization of eae+ Escherichia coli isolated from healthy and diarrheic calves

Holland

Wilson²,

Holland

et al. 1999

Veterinary Microbiology

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Strains of Escherichia coli from 101 healthy and 114 diarrheic calves were screened by PCR for the eae (intimin) gene and Shiga toxin genes (stx). Each eae+ and eae/stx+ strain was examined for antimicrobial susceptibility, enterohemolysin activity, and the somatic O antigen was determined. An immunoassay was used to detect Shiga toxin antigens for the eae/stx+ E. coli. Significantly more (p = 0.005) of the healthy calves carried eae+ and eae/stx+ E. coli in their feces when compared to strains from diarrheic calves. Moreover, Shiga toxin antigens were detected significantly more (p = 0.001) often among the eae/stx+ strains from healthy calves when compared to eae/stx+ strains from diarrheic calves. However, significantly more (p = 0.001) of the eae+ and eae/stx+ strains from diarrheic calves were resistant to at least one of the antimicrobials tested, and the strains from diarrheic calves had a significantly (p = 0.05) higher rate of antimicrobial resistance to at least two different antimicrobial classes. No significant difference (p> or =0.05) was detected among the eae+ and eae/stx+ strains from healthy and diarrheic calves for enterohemolysin production. Serogroups O-negative, O5, O26, and O111 were predominate among both healthy and diarrheic calves.

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“…Serotyping was performed on 4 of the 6 strains using standard methods at the Escherichia coli Reference Center, Pennsylvania State University. 8,25 The 6 animals examined were presented to the Veterinary Medical Center at Michigan State University for medical care. At the time of fecal collection, 5 of the 6 animals had diarrhea ( Table 1).…”

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confidence: 99%