Serotypes, virulence genes, intimin types and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia

Vu-Khac, Hung; Holoda, E.; Pilipčinec, E.; Blanco, Miguel; Dahbi, Ghizlane; Mora, Azucena; López, Cecilia; González, Enrique A.; Blanco, Jesús E.

doi:10.1016/j.tvjl.2006.05.019

Cited by 100 publications

(104 citation statements)

References 47 publications

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“…This is in accordance with the Swiss situation about 15 years ago [3] and also with other studies from Europe and the US [4,5,11]. The fimbrial gene for F4 was detected uniquely in ETEC isolates and was the predominant adhesion factor (77%).…”

Section: Etecsupporting

confidence: 91%

Aetiologically Relevant Typing of E. Coli Isolates from Diseased Pigs in Switzerland during 2014 and 2015

Schneeberger¹,

Dietz²,

Kirchgässner³

et al. 2017

ARC Journal of Animal and Veterinary Sciences

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Section: Etecsupporting

confidence: 91%

Aetiologically Relevant Typing of E. Coli Isolates from Diseased Pigs in Switzerland during 2014 and 2015

Schneeberger¹,

Dietz²,

Kirchgässner³

et al. 2017

ARC Journal of Animal and Veterinary Sciences

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“…The PFGE method is a sensitive molecular method to analyze the degree of genetic relatedness or variability among porcine ETEC strains. [21][22][23] To the authors' knowledge, genetic relatedness compared with virulent and antibiotic patterns of ETEC from pigs have never been reported in Korea. Therefore, the purpose of the current study is to examine virulence genotype, antibiogram, and PFGE profiles of ETEC strains.…”

Section: Adherence Factors (F1 [Type 1 Fimbriae] F4 [K88] F5mentioning

confidence: 99%

Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

et al. 2009

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Abstract. Adherence factors and enterotoxins are major virulence factors of enterotoxigenic Escherichia coli (ETEC). Antibiotics have been used frequently for the treatment and prevention of ETEC infection in piggeries worldwide, including Korea. Therefore, data on both virulence profiles and antibiotic resistance patterns are useful in the epidemiological study of ETEC. A total number of 198 E. coli field isolates were examined. The most prevalent pathotype was F1, followed by a combination of F1 and EAST1. All of the 71 isolates were resistant to more than 2 antibiotics used in a disk diffusion test, and 87.94% of the isolates were found to be resistant to more than 4 antibiotics. Investigations were also conducted to correlate the virulence gene profiles with antibiogram and pulsed-field gel electrophoresis (PFGE). Although a high degree of polymorphism was noted among strains having the same virulence patterns, the highest similarity pattern was observed carrying the same virulence profiles and similar antibiogram. Thus, investigation of both virulence profiles and antibiogram is essential to the epidemiological study of ETEC. Moreover, the PFGE method might be applicable as a tool to reveal genetic relatedness among E. coli strains from piggeries in Korea.

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“…Therefore, impairing this ability represents an ideal defense approach because of its importance in the early stages of the infectious process (Cegelski et al 2008;Savar et al 2014). The pathogenicity of ETEC also depends on its ability to adhere to and colonize the intestinal epithelium (Vu-Khac et al 2007). Adhesins facilitate attachment of ETEC to specific receptors on mucosal epithelial cells and the adjacent mucous layer of the small intestine (Vu-Khac et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…The pathogenicity of ETEC also depends on its ability to adhere to and colonize the intestinal epithelium (Vu-Khac et al 2007). Adhesins facilitate attachment of ETEC to specific receptors on mucosal epithelial cells and the adjacent mucous layer of the small intestine (Vu-Khac et al 2007). In particular, ETEC F4 (K88), F5 (K99), and F6 (987P or Fas) fimbria facilitate adhesion in neonatal piglets (Kim et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Hur

Kim

et al. 2015

Veterinary Quarterly

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Results: All groups with single, double, and triple inoculations of the ghost showed higher humoral and mucosal immune responses than the control group. In particular, the groups with intramuscular double and triple inoculations showed significantly higher immune responses. In addition, oral inoculation with a combination of the ghost and MONTANIDE IMS 1113 (MI1113) resulted in high and prolonged induction of intestinal IgA levels. Conclusion: These results indicated that both systemic and mucosal immunity against ETEC fimbrial antigens expressed by the ghost are induced by intramuscular booster inoculation with the ghost, and that addition of M1113 to the ghost was found to result in prominent induction of mucosal immunity through oral inoculation.

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Serotypes, virulence genes, intimin types and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia

Cited by 100 publications

References 47 publications

Aetiologically Relevant Typing of E. Coli Isolates from Diseased Pigs in Switzerland during 2014 and 2015

Aetiologically Relevant Typing of E. Coli Isolates from Diseased Pigs in Switzerland during 2014 and 2015

Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

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