Serotyping of Campylobacter jejuni based on heat stable antigens: relevance, molecular basis and implications in pathogenesis

Moran, Anthony P.; Penner, J L

doi:10.1046/j.1365-2672.1999.00713.x

Cited by 62 publications

(54 citation statements)

References 120 publications

(205 reference statements)

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“…(29); however, the additional presence in some strains of ladder-like high-molecular-weight molecules was at first thought to suggest that such strains also produced high-molecular-weight O-chain-bearing LPS (38). The structural similarity of some of the polysaccharide repeat units to those found in other gram-negative O-chains supported the assumption that the ladder-like polysaccharide was the O-chain and, thus, was linked to the core oligosaccharide (29). Although there were limited structural data to support the covalent attachment of an O-chain polysaccharide to a lipid A core molecule (3), there were data from structural studies to suggest the production of other types of extracellular polysaccharide molecules (2,15).…”

Section: Discussionmentioning

confidence: 52%

“…Thus, although there is a greater capacity for polysaccharide biosynthesis than previously supposed, C. jejuni may only express LOS and not LPS. C. jejuni heat-stable antigens, considered to include a capsule and LOS, form the basis of the Penner serotyping system (18,29,38). Certain serotypes, and hence LOS structures, have been linked to the development of C. jejuni-associated GBS or MFS (37).…”

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confidence: 99%

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Characterization of theCampylobacter jejuniHeptosyltransferase II Gene,waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent

et al. 2002

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Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurological complications. In Escherichia coli and Salmonella enterica serovar Typhimurium the waaF gene encodes a heptosyltransferase, which catalyzes the transfer of the second L-glycero-D-manno-heptose residue to the core oligosaccharide moiety of lipopolysaccharide (LPS), and mutation of waaF results in a truncated core oligosaccharide. In this report we confirm experimentally that C. jejuni gene Cj1148 encodes the heptosyltransferase II enzyme, WaaF. The Campylobacter waaF gene complements an S. enterica serovar Typhimurium waaF mutation and restores the ability to produce full-sized lipopolysaccharide. To examine the role of WaaF in C. jejuni, waaF mutants were constructed in strains NCTC 11168 and NCTC 11828. Loss of heptosyltransferase activity resulted in the production of a truncated core oligosaccharide, failure to bind specific ligands, and loss of serum reactive GM 1 , asialo-GM 1 , and GM 2 ganglioside epitopes. The mutation of waaF did not affect the higher-molecular-weight polysaccharide supporting the production of a LOS-independent capsular polysaccharide by C. jejuni. The exact structural basis for the truncation of the core oligosaccharide was verified by comparative chemical analysis. The NCTC 11168 core oligosaccharide differs from that known for HS:2 strain CCUG 10936 in possessing an extra terminal disaccharide of galactose-␤(1,3) N-acetylgalactosamine. In comparison, the waaF mutant possessed a truncated molecule consistent with that observed with waaF mutants in other bacterial species.

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Section: Discussionmentioning

confidence: 52%

mentioning

confidence: 99%

Characterization of theCampylobacter jejuniHeptosyltransferase II Gene,waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent

et al. 2002

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“…Recent studies have shown the existence of two distinct HS antigens in Campylobacter, which may account for the differences in the two schemes. These antigens are a polysaccharide capsule (3,13) and a lipopolysaccharide and/or lipooligosaccharide component of the cell surface (17). The majority of C. jejuni isolates belong to only a limited number of HS serotypes, and 19% of isolates are nontypeable with the current antiserum panel using the direct agglutination method.…”

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confidence: 99%

Genome Sequence-Based Fluorescent Amplified Fragment Length Polymorphism of Campylobacter jejuni , Its Relationship to Serotyping, and Its Implications for Epidemiological Analysis

Desai

Logan

Frost³

et al. 2001

J Clin Microbiol

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The published genome sequence of Campylobacter jejuni strain NCTC 11168 was used to model an accurate and highly reproducible fluorescent amplified fragment length polymorphism (FAFLP) analysis. Predicted and experimentally observed amplified fragments (AFs) generated with the primer pair HindIII؉A and HhaI؉A were compared. All but one of the 61 predicted AFs were reproducibly detected, and no unpredicted fragments were amplified. This FAFLP analysis was used to genotype 74 C. jejuni strains belonging to the nine heat-stable (HS) serotypes most prevalent in human disease in England and Wales. The 74 C. jejuni strains exhibited 60 FAFLP profiles, and cluster analysis of them yielded a radial tree showing genetic relationships between and within 13 major clusters. Some clusters were related, and others were unrelated, to a single HS serotype. For example, all strains belonging to serotypes HS6 and HS19 grouped into corresponding single genotypic clusters, while strains of serotypes HS11 and HS18 each grouped into two genotypic clusters. Strains of HS50, the most prevalent serotype infecting humans, were found both in one large (multiserotype) cluster complex and dispersed throughout the tree. The strain genotypes within each FAFLP cluster were characterized by a particular combination of AFs, and among the cluster there were additional differential AFs. Identification of such AFs could act as a search tool to look for potential associations with disease or animal hosts, when applied to large number of human isolates. Genome-sequence based FAFLP, thus, has the potential to establish a genetic database for epidemiological investigations of Campylobacter.

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“…The former is the most widely accepted and well evaluated phenotypic method. The molecular basis for the HS antigenic diversity in C. jejuni and C. coli is the expression of somatic (O) lipopolysaccharide (LPS) (17,18,21,22,33,34,35). LPS is a major constituent of the outer membrane in gram-negative bacteria and comprises three covalently linked regions: lipid A, core oligosaccharide (inner core and outer core), and O polysaccharide.…”

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confidence: 99%

Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis

et al. 2002

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A molecular typing approach for Campylobacter jejuni and Campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb PCR-amplified portion of the lipopolysaccharide gene cluster. Sixty-one Penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. Eleven additional genogroups were obtained from 87 clinical C. jejuni strains tested. This molecular typing method shows a correlation with the Penner heat-stable serotyping method, a phenotypic typing method based on lipopolysaccharide structures that is often used as a "gold standard" for subtyping Campylobacter spp. This strong correlation suggests that the data obtained can be directly compared with epidemiological data collected in the past by classical serotyping of C. jejuni and C. coli. In contrast to the high percentage of nontypeability by phenotyping, this molecular typing method results in 100% typeability and provides a superior alternative to serotyping.

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Serotyping of Campylobacter jejuni based on heat stable antigens: relevance, molecular basis and implications in pathogenesis

Cited by 62 publications

References 120 publications

Characterization of theCampylobacter jejuniHeptosyltransferase II Gene,waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent

Characterization of theCampylobacter jejuniHeptosyltransferase II Gene,waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent

Genome Sequence-Based Fluorescent Amplified Fragment Length Polymorphism of Campylobacter jejuni , Its Relationship to Serotyping, and Its Implications for Epidemiological Analysis

Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis

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