Serotyping of pneumococci: evaluation of the genetic approach and performance with clinical samples

Burckhardt, Irene; Roemer, Johanna; Burckhardt, Florian; Zimmermann, Stefan

doi:10.1016/j.diagmicrobio.2014.08.013

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…After thawing of each strain, its serotype was confirmed by a serotype-specific PCR before the isolate was used for database generation [18, 19]. …”

Section: Methodsmentioning

confidence: 99%

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Analysis of Streptococcus pneumoniae using Fourier-transformed infrared spectroscopy allows prediction of capsular serotype

Burckhardt

Sebastian

Mauder

et al. 2019

Eur J Clin Microbiol Infect Dis

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Determination of the capsule type of clinical isolates of Streptococcus pneumoniae is a prerequisite for epidemiological studies and further vaccine development. The Quellung reaction for serotyping is expensive and mostly done in reference centres. We wanted to evaluate whether Fourier-transformed infrared (FT-IR) spectroscopy is suitable for capsular type analysis and prediction of pneumococcal serotypes. We used the IR-Biotyper™ (Bruker) to create a database containing the spectra of 120 strains from invasive disease. The strains covered the 24 vaccine serotypes contained in the 13-valent conjugate vaccine (PCV13) and the 23-valent polysaccharide vaccine (PSV23). Hierarchical clustering analysis was performed. Finally, two different classification sets were created (PCV13 and PSV23). They were used to predict the serotype of 168 different challenge strains (invasive and non-invasive disease) covering 48 different serotypes (vaccine and non-vaccine types). FT-IR spectra from pneumococci (1300–800 cm−1) clustered along their serotype as determined by the Quellung reaction (120 strains, 24 different serotypes). Strains with unknown serotype fell within the cluster of the correct serotype, as long as the latter was represented in the database (168 strains, 48 different serotypes). Concordance between the Quellung reaction and FT-IR spectroscopy was excellent (kappa ≥ 0.75). FT-IR spectroscopy is a fast and cost-effective method to predict the capsular serotype of pneumococci.Electronic supplementary materialThe online version of this article (10.1007/s10096-019-03622-y) contains supplementary material, which is available to authorized users.

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“…After thawing of each strain, its serotype was confirmed by a serotype-specific PCR before the isolate was used for database generation [18, 19]. …”

Section: Methodsmentioning

confidence: 99%

“…All pneumococcal strains used for database creation and classification dataset creation had been previously serotyped (Quellung reaction). After thawing of each strain, its serotype was confirmed by a serotype-specific PCR before the isolate was used for database generation [18, 19].…”

Section: Methodsmentioning

confidence: 99%

Analysis of Streptococcus pneumoniae using Fourier-transformed infrared spectroscopy allows prediction of capsular serotype

Burckhardt

Sebastian

Mauder

et al. 2019

Eur J Clin Microbiol Infect Dis

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“…In this context, very few dimethoxytriazines have been described [24][25][26][27][28][29][30][31][32][33][34], and some of their 2-(alk-1 0 -ynyl) derivatives such as I-III [35] present interesting cytotoxic activities toward different tumor cell lines (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and biological evaluation of a new class of triazin–triazoles as potential inhibitors of human farnesyltransferase

et al. 2015

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A new synthesis of ethynyldimethoxytriazine 1, an important platformcompound for developing new chemical entities for anticancer research and for other biological applications, is described. Compound 1 was further reacted with azides 5a-i to provide triazin-triazoles 2a-i, which were tested on human farnesyltransferase and on the NCI-60 human tumor cell lines. Synthesis of other dimethoxytriazine derivatives 15 and 16, linked to a sp 2 or a sp 3 carbon atom were also studied. Electronic supplementary material The online version of this article (2a-i target compounds CO 2 H 2c: IC 50 (human FTase) = 38.6 µM

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Streptococcus pneumoniae as an agent of urinary tract infections – a laboratory experience from 2010 to 2014 and further characterization of strains

Burckhardt

Panitz

Linden

et al. 2016

Diagnostic Microbiology and Infectious Disease

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Serotyping of pneumococci: evaluation of the genetic approach and performance with clinical samples

Cited by 5 publications

References 17 publications

Analysis of Streptococcus pneumoniae using Fourier-transformed infrared spectroscopy allows prediction of capsular serotype

Analysis of Streptococcus pneumoniae using Fourier-transformed infrared spectroscopy allows prediction of capsular serotype

Synthesis and biological evaluation of a new class of triazin–triazoles as potential inhibitors of human farnesyltransferase

Streptococcus pneumoniae as an agent of urinary tract infections – a laboratory experience from 2010 to 2014 and further characterization of strains

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