Serovars and SpaA Types of Erysipelothrix rhusiopathiae Isolated from Pigs in Japan from 2012 to 2019

Morimoto, Misako; Kato, Atsushi; Kojima, Hiroe; Akaike, Yuta; Nogami, Kotoe; Sasakawa, Chihiro; Nagai, Shinya; To, Ho

doi:10.1007/s00284-020-02254-2

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…The Fujisawa strain was used as the reference strain of E. rhusiopathiae [ 18 ]. One of the major SpaA-type variants having M203/I257 SpaA-type, 2012 Miyazaki variant that was isolated previously [ 15 , 16 ], was representatively used. The Fujisawa strain and 2012 Miyazaki variant belong to serovar 1a variant.…”

Section: Methodsmentioning

confidence: 99%

“…Marienfelde strain was cultured in BHI medium [ 16 ]. Colony-forming units (CFU) of Fujisawa strain and 2012 Miyazaki variant were measured by counting the colonies on TPB agar plates as previously described [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Importantly, the outbreaks in Japan from 2008 to 2011 were caused mainly by serovar 1a (95.2%), and among these, 96.2% was M203/I257 SpaA-type variant [ 13 ]. Predominance of this variant (74.6%) was still observed in strains isolated during the outbreak in Japan from 2012 to 2019 [ 15 ]. This indicates that the serovar 1a M203/I257 SpaA-type variant of E. rhusiopathiae is the major causative strain of SE as ever in Japan.…”

Section: Introductionmentioning

confidence: 99%

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The Swine Erysipelas Vaccine SER-ME Effectively Protects Pigs against Challenge with the Erysipelothrix rhusiopathiae M203/I257 SpaA-Type Variant

Morimoto

Kato

Nogami

et al. 2022

Veterinary Sciences

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Erysipelothrix rhusiopathiae causes swine erysipelas (SE). Sporadic SE outbreaks in Japan are mostly caused by the E. rhusiopathiae serovar 1a variant featured by methionine (M) and isoleucine (I) at amino acid positions 203 and 257 of the surface protective antigen (Spa) A protein (M203/I257 SpaA-type). To determine if current vaccines are effective against infection with this variant in pigs, one representative inactivated vaccine, SER-ME (containing E. rhusiopathiae serovar 2a), was evaluated. All vaccinated pigs survived without any apparent clinical signs after lethal challenge with the Fujisawa reference strain or the variant. This indicates that the SER-ME vaccine effectively protects pigs against the infection of E. rhusiopathiae M203/I257 SpaA-type variant. Current vaccines in Japan, including SER-ME, suggest that outbreaks in Japan are unlikely caused by vaccine failure.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Swine Erysipelas Vaccine SER-ME Effectively Protects Pigs against Challenge with the Erysipelothrix rhusiopathiae M203/I257 SpaA-Type Variant

Morimoto

Kato

Nogami

et al. 2022

Veterinary Sciences

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“…An outbreak of acute swine erysipelas was reported in Eastern China, in which seven other E. rhusiopathiae strains were isolated since the summer of 2013 [15]. In Japan, approximately 2000 cases of erysipelas in pigs are reported per year, with sporadic outbreaks of the disease reported from 2008 to 2019 mainly caused by the Met-203 type strain, suggesting that this strain might be widespread in the country [16][17][18]. Due to the high frequency of occurrence of these outbreaks, there is a suspicion that the vaccine composed of the attenuated bacterium may have its virulence reversed.…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and Protection by DnaK and SpaA Recombinant Proteins Against Erysipelothrix Rhusiopathiae in a Murine Model

2023

Cell Physiol Biochem

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Background/Aims: Swine erysipelas is a disease caused by Erysipelothrix rhusiopathiae , a Gram-positive bacillus, which has great economic importance because it leads to the loss of the swine herd. To control this disease, animals are immunized with a cellular vaccine of killed or attenuated E. rhusiopathiae , but even with herd vaccination, cases of swine erysipelas outbreaks have been reported in the United States, China and Japan, leading to the search for other antigenic components of the bacteria that may promote greater protection against E. rhusiopathiae . The surface protein SpaA from E. rhusiopathiae has been shown to be a candidate to constitute a subunit vaccine, since it has already been reported to induce a host immune response against the bacterium. DnaK, a hsp70 molecular chaperone, also seems to be a good candidate in the composition of a vaccine, as it has been demonstrated to be an antigenic protein of the bacteria. Methods: This work evaluated the immunogenicity and protection induced by the E. rhusiopathiae SpaA and DnaK recombinant proteins in a murine model, by intramuscular administration to mice with two doses of 100 µg at 21-day interval between them. The candidate proteins were tested either separately and together, compared with the commercial vaccine and the non-vaccination condition, and mice were challenged with a virulent strain of E. rhusiopathiae. Serum was collected to assess the produced antibodies and peripheral blood cells, whereas spleen and kidney tissues were assayed for E. rhusiopathiae presence by colony counting. Results: A survival curve of the animals was performed, which confirmed the protection induced by the proteins. IgG antibodies increased in the animal serum inoculated with the proteins when compared to the control, and a significant delay in disease symptoms was observed. Conclusion: These results suggest that E. rhusiopathiae DnaK and SpaA are immunogenic in mice and interfere with the disease development.

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Comparative study of the phenotype and virulence of recent serovar 1a, 1b, and 2a isolates of Erysipelothrix rhusiopathiae in Japan

Morimoto

Kato

Akaike

et al. 2022

Veterinary Microbiology

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Serovars and SpaA Types of Erysipelothrix rhusiopathiae Isolated from Pigs in Japan from 2012 to 2019

Cited by 5 publications

References 27 publications

The Swine Erysipelas Vaccine SER-ME Effectively Protects Pigs against Challenge with the Erysipelothrix rhusiopathiae M203/I257 SpaA-Type Variant

The Swine Erysipelas Vaccine SER-ME Effectively Protects Pigs against Challenge with the Erysipelothrix rhusiopathiae M203/I257 SpaA-Type Variant

Immunogenicity and Protection by DnaK and SpaA Recombinant Proteins Against Erysipelothrix Rhusiopathiae in a Murine Model

Comparative study of the phenotype and virulence of recent serovar 1a, 1b, and 2a isolates of Erysipelothrix rhusiopathiae in Japan

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