SERPINB3 (Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3)

Turato, Cristian; Pontisso, Patrizia

doi:10.4267/2042/56413

Cited by 18 publications

(32 citation statements)

References 61 publications

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“…In the 1970s, Kato and Torigoe isolated squamous cell carcinoma‐specific antigen from cervical squamous cell carcinoma. The antigen is composed of two isomers with almost the same molecular weight, S ERPINB3 and SERPINB4, among which SERPINB3 is a serine protease inhibitor . SERPINB3 is highly expressed in mucous epithelial squamous cell carcinoma of the cervix, esophagus, head and neck, oral cavity, liver, etc, but not expressed or expressed at a low level in normal human squamous cells .…”

Section: Discussionmentioning

confidence: 99%

Study on the targeted therapy of oral squamous cell carcinoma with a plasmid expressing PE38KDEL toxin under control of the SERPINB3 promoter

et al. 2020

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Oral squamous cell carcinoma (OSCC) has a poor prognosis and a high risk of recurrence. To improve the efficacy of OSCC therapy, it is of great significance to explore gene therapy for OSCC. The use of specific genes to regulate the targeted expression of suicide genes is a hot topic in gene therapy for cancer. The SERPINB3 gene is highly active in squamous cell carcinoma, but nearly undetectable or present at a low level in normal tissues. This specificity suggests that the SERPINB3 promoter can be used for targeted OSCC therapy. Pseudomonas aeruginosa secretes PE38KDEL, an exotoxin derivative, as a suicide gene used in gene therapy. A SERPINB3 promoter‐mediated PE38KDEL expression vector was created. The SERPINB3 gene expression was tested in different cell lines by RT‐qPCR and Western blotting, and the SERPINB3 promoter activity was detected by luciferase assay. The SERPINB3 promoter was more active in the TCA8113 cell line than in the other cell lines. The target therapeutic potential of the toxin vector pSERPINB3‐PE38KDEL was tested in the SERPINB3‐positive TCA8113 cell line, the SERPINB3‐negative MG63 cell line, and normal L02 cell line. The SERPINB3 gene was expressed at a high level in TCA8113 cells but a low level in MG63 and L02 cells. Transfection of the pSERPINB3‐PE38KDEL plasmid effectively inhibited the proliferation and invasion of TCA8113 cells and induced cell apoptosis, but no significant damage to MG63 and L02 cells was observed. The results of in vitro experiments indicated that the pSERPINB3‐PE38KDEL plasmid could be a promising strategy for targeted OSCC gene therapy.

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Section: Discussionmentioning

confidence: 99%

Study on the targeted therapy of oral squamous cell carcinoma with a plasmid expressing PE38KDEL toxin under control of the SERPINB3 promoter

et al. 2020

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“…Recent findings indicate that SCCA1 is upregulated during Barrett's carcinogenesis and predicts EAC resistance to neoadjuvant chemotherapy . In fact, several studies have highlighted a significant association between SCCA1 overexpression and poor cancer prognosis, also associated with chemoresistance in different tumor types . Squamous cell carcinoma antigen‐1 confers to cancer cells resistance inhibiting apoptosis and reactive oxygen species generation and cell death .…”

Section: Discussionmentioning

confidence: 99%

“…More recently, the role of SCCA1‐1 has been reported in inhibiting reactive oxygen species generation and cell death through its inhibitory interaction with respiratory complex I . In addition, these and other studies have highlighted a significant association between SCCA1‐1 overexpression and poor prognosis, also associated with chemoresistance in different tumor types …”

Section: Introductionmentioning

confidence: 86%

“…Recent findings indicate that squamous cell carcinoma antigen‐1 (SCCA1) is upregulated during Barrett's carcinogenesis and predicts EAC resistance to neoadjuvant chemotherapy . Squamous cell carcinoma antigen‐1 is a serine protease inhibitor that belongs to the clade B subset of serpins and includes the 2 isoforms SCCA1‐1 (also known as SerpinB3) and SCCA1‐2 (also known as SerpinB4) . The first described isoform, SCCA1‐1, was initially found to be significantly overexpressed in carcinomas with squamous differentiation, but recent evidence also extends to adenocarcinoma of the lung, breast, and pancreas, as well as hepatocellular carcinoma .…”

Section: Introductionmentioning

confidence: 99%

“…The first described isoform, SCCA1‐1, was initially found to be significantly overexpressed in carcinomas with squamous differentiation, but recent evidence also extends to adenocarcinoma of the lung, breast, and pancreas, as well as hepatocellular carcinoma . The SCCA1‐1 isoform confers to cancer cells resistance to induced apoptosis by different mechanisms, including inhibition of lysosomal cathepsin proteases, the JNK pathway, or p38 activation . More recently, the role of SCCA1‐1 has been reported in inhibiting reactive oxygen species generation and cell death through its inhibitory interaction with respiratory complex I .…”

Section: Introductionmentioning

confidence: 99%

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Squamous cell carcinoma antigen 1 is associated to poor prognosis in esophageal cancer through immune surveillance impairment and reduced chemosensitivity

et al. 2019

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Squamous cell carcinoma antigen‐1 ( SCCA 1) overexpression is associated with poor prognosis and chemoresistance in several tumor types, however, the underlying mechanisms remain elusive. Here, we report SCCA 1 in relation to the immune and peritumoral adipose tissue microenvironment in early and advanced esophageal adenocarcinoma ( EAC ). In our series of patients with EAC, free SCCA 1 serum levels were associated with significantly worse overall survival, and SCCA 1‐IgM serum levels showed a trend to a worse overall survival. Serum SCCA 1 and intratumoral SCCA 1 were inversely correlated with immune activation markers. In agreement with these findings, SCCA 1 induced the expression of the immune checkpoint molecule programmed death ligand‐1 on monocytes and a direct correlation of these 2 molecules was observed in sequential tumor sections. Furthermore, SCCA 1 mRNA expression within the tumor was inversely correlated with stem cell marker expression both within the tumor and in the peritumoral adipose tissue. In vitro, in EAC cell lines treated with different chemotherapeutic drugs, cell viability was significantly modified by SCCA 1 presence, as cells overexpressing SCCA 1 were significantly more resistant to cell death. In conclusion, poor prognosis in EAC overexpressing SCCA 1 is due to reduced tumor chemosensitivity as well as intratumoral immunity impairment, likely induced by this molecule .

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Multivariate investigation of aging in mouse models expressing the Alzheimer’s protective APOE2 allele: integrating cognitive metrics, brain imaging, and blood transcriptomics

Moon,

Mahzarnia,

Stout

et al. 2023

Brain Struct Funct

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SERPINB3 (Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3)

Abstract: Review on SERPINB3, with data on DNA/RNA, on the protein encoded and where the gene is implicated.

Cited by 18 publications

References 61 publications

Study on the targeted therapy of oral squamous cell carcinoma with a plasmid expressing PE38KDEL toxin under control of the SERPINB3 promoter

Study on the targeted therapy of oral squamous cell carcinoma with a plasmid expressing PE38KDEL toxin under control of the SERPINB3 promoter

Squamous cell carcinoma antigen 1 is associated to poor prognosis in esophageal cancer through immune surveillance impairment and reduced chemosensitivity

Multivariate investigation of aging in mouse models expressing the Alzheimer’s protective APOE2 allele: integrating cognitive metrics, brain imaging, and blood transcriptomics

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