Serratia inhibens sp. nov., a new antifungal species isolated from potato (Solanum tuberosum)

Abstract

A novel bacterial strain, S40T, with strong antifungal activity was isolated from the rhizosphere of green potato collected from Zealand, Denmark. Polyphasic analysis with a combined phenotypic, phylogenetic and genomic approach was used to characterize S40T. Phylogenetic analysis based on the 16S rRNA gene and MLSA (concatenated gyrB, rpoD, infB and atpD sequences) showed that strain S40T was affiliated with the genus Serratia and with Serratia plymuthica PRI-2C as the closest related strain [average nucleotide identity (ANI), 99.26 %; DNA–DNA hybridization (dDDH), 99.20%]. However, whole genome sequence analyses revealed that S40T and S. plymuthica PRI-2C genomes displayed lower similarities when compared to all other S. plymuthica strains (ANI ≤94.34 %; dDDH ≤57.6 % relatedness). The DNA G+C content of strain S40T was determined to be 55.9 mol%. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobic and displayed growth at 10–37 °C (optimum, 25–30 °C) and at pH 6–9 (optimum, pH 6–7). Major fatty acids were C16 : 0 (27.9 %), summed feature (C16 : 1 ω6c/C16 : 1 ω7c; 18.0 %) and C17 : 0 cyclo (15.1 %). The respiratory quinone was determined to be Q8 (94 %) and MK8 (95 %) and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The results of phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S40T represents a novel species of the genus Serratia , for which the name Serratia inhibens sp. nov. is proposed. The type strain is S40T (=LMG 31467T=NCIMB 15235T). In addition, we propose that S. plymuthica PRI-2C is reclassified and transferred to the species S. inhibens as S. inhibens PRI-2C.

Local Network Properties of Soil and Rhizosphere Microbial Communities in Potato Plantations Treated with a Biological Product Are Important Predictors of Crop Yield

“…It is necessary to keep in mind that the predictive power of PC variables, as principal components of a multivariate analysis, came from the interaction patterns among the OTUs and not from their individual behavior. However, we can highlight the presence, for instance, of the bacterial biocontrol-related genus Serratia ( 42 ) as the taxonomic identity of the OTU with the highest positive correlation with yield in PC25 and Bacillus sp. as the taxonomic identity of the OTU with the highest positive correlation with yield in PC3 ( Fig.…”

Genome of Serratia plymuthica UBCF_13, Insight into diverse unique traits

“…The phylogenetic tree shows that S. plymuthica UBCF_13 is in same cluster with strain AS9, PRI-2C, NCTC8015, and NCTC8900. The strain PRI-2C is reclassified and transferred to the species S. inhibens 22 . The pangenome was performed together with other strains in order to obtain further insight into specific features in the UBCF_13.…”

Pseudomonas taxonomic and functional microdiversity in the wheat rhizosphere is cultivar-dependent and links to disease resistance profile and root diameter

“…A positive result required a clearance zone of at least 1 mm around a colony. Serratia inhibens S40 (Hennessy et al, 2020) was used as the positive control.…”