SERRS as a more sensitive technique for the detection of labelled oligonucleotides compared to fluorescence

Faulds, Karen; Barbagallo, Romina Paola; Keer, Jacquie T.; Smith, W. Ewen; Graham, Duncan

doi:10.1039/b406423b

Cited by 126 publications

(125 citation statements)

References 12 publications

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“…Surface-enhanced Raman spectroscopy is a plasmonic effect whereby molecules adsorbed onto nanoroughened noble metal surfaces show a dramatic increase in the incident electromagnetic field, resulting in high Raman intensities. 23 Figure 4 shows the spectra of the different blood samples and confirms the absence of SWCNT in the blood samples. The absence of SWCNT in the blood 24 hours after injection is in accordance with reported studies showing that the blood circulation of SWCNT is limited to a few hours (16 hours maximum), and can vary with the type of nanotubes used and their functionalization.…”

Section: Ex Vivo Analysismentioning

confidence: 92%

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In vivo biodistribution and biological impact of injected carbon nanotubes using magnetic resonance techniques

Faraj¹,

Fauvelle²,

Luciani³

et al. 2011

IJN

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International audienceSingle-walled carbon nanotubes (SWCNT) hold promise for applications as contrast agents and target delivery carriers in the field of nanomedicine. When administered in vivo, their biodistribution and pharmacological profile needs to be fully characterized. The tissue distribution of carbon nanotubes and their potential impact on metabolism depend on their shape, coating, and metallic impurities. Because standard radiolabeled or fluorescentlylabeled pharmaceuticals are not well suited for long-term in vivo follow-up of carbon nanotubes, alternative methods are required

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Section: Ex Vivo Analysismentioning

confidence: 92%

“…22,23 Volumes as low as 10 µL of homogenized blood were put on a gold-coated silicon surface (Mesophotonics United, Southampton, UK) to enhance the Raman signal, and the spectra were acquired using the same parameters as described above.…”

Section: Blood Raman Measurementsmentioning

confidence: 99%

In vivo biodistribution and biological impact of injected carbon nanotubes using magnetic resonance techniques

Faraj¹,

Fauvelle²,

Luciani³

et al. 2011

IJN

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“…IIIE for details on these types of imaging agents). In brief, Raman-active nanoparticles (or SERS nanoparticles) are known to significantly enhance the Raman scattering efficiency (221) and thus improve the detection limits (sensitivity) of the technique to around three orders of magnitude lower than that achievable via fluorescence imaging (101). By administering different SERS nanoparticles (FIGURE 10A), one can theoretically visualize several targets simultaneously.…”

Section: Raman Imagingmentioning

confidence: 99%

A Molecular Imaging Primer: Modalities, Imaging Agents, and Applications

James

Gambhir

2012

Physiological Reviews

967

954

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Molecular imaging is revolutionizing the way we study the inner workings of the human body, diagnose diseases, approach drug design, and assess therapies. The field as a whole is making possible the visualization of complex biochemical processes involved in normal physiology and disease states, in real time, in living cells, tissues, and intact subjects. In this review, we focus specifically on molecular imaging of intact living subjects. We provide a basic primer for those who are new to molecular imaging, and a resource for those involved in the field. We begin by describing classical molecular imaging techniques together with their key strengths and limitations, after which we introduce some of the latest emerging imaging modalities. We provide an overview of the main classes of molecular imaging agents (i.e., small molecules, peptides, aptamers, engineered proteins, and nanoparticles) and cite examples of how molecular imaging is being applied in oncology, neuroscience, cardiology, gene therapy, cell tracking, and theranostics (therapy combined with diagnostics). A step-by-step guide to answering biological and/or clinical questions using the tools of molecular imaging is also provided. We conclude by discussing the grand challenges of the field, its future directions, and enormous potential for further impacting how we approach research and medicine.

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“…The discovery of the surface enhanced Raman scattering (SERS) phenomenon offers an exciting opportunity to overcome this lack of sensitivity and introduce Raman spectroscopy into new fields (8). SERS is a plasmonic effect where molecules adsorbed onto nano-roughened noble metal surfaces experience a dramatic increase in the incident electromagnetic field, resulting in high Raman intensities comparable to fluorescence (6).…”

mentioning

confidence: 99%

“…Fluorescence imaging, in particular, has significant potential for in vivo studies but is limited by several factors (5,6), including a limited number of fluorescent molecular imaging agents available in the near infra-red (NIR) window with large spectral overlap between them, which restricts the ability to interrogate multiple targets simultaneously (multiplexing). In addition, background autofluorescence emanating from superficial tissue layers restricts the sensitivity and the depth to which fluorescence imaging can be used.…”

mentioning

confidence: 99%

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Keren

Zavaleta

Cheng

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

563

475

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Molecular imaging of living subjects continues to rapidly evolve with bioluminescence and fluorescence strategies, in particular being frequently used for small-animal models. This article presents noninvasive deep-tissue molecular images in a living subject with the use of Raman spectroscopy. We describe a strategy for small-animal optical imaging based on Raman spectroscopy and Raman nanoparticles. Surface-enhanced Raman scattering nanoparticles and single-wall carbon nanotubes were used to demonstrate whole-body Raman imaging, nanoparticle pharmacokinetics, multiplexing, and in vivo tumor targeting, using an imaging system adapted for small-animal Raman imaging. The imaging modality reported here holds significant potential as a strategy for biomedical imaging of living subjects.nanotubes ͉ SERS nanoparticles M olecular imaging of living subjects provides the ability to study cellular and molecular processes that have the potential to impact many facets of biomedical research and clinical patient management (1-4). Imaging of small-animal models is currently possible by using positron emission tomography (PET), single photon emission computed tomography, magnetic resonance imaging, computed tomography, optical bioluminescence and fluorescence, high frequency ultrasound, and several other emerging modalities. However, no single modality currently meets the needs of high sensitivity, high spatial and temporal resolution, high multiplexing capacity, low cost, and high-throughput.Fluorescence imaging, in particular, has significant potential for in vivo studies but is limited by several factors (5, 6), including a limited number of fluorescent molecular imaging agents available in the near infra-red (NIR) window with large spectral overlap between them, which restricts the ability to interrogate multiple targets simultaneously (multiplexing). In addition, background autofluorescence emanating from superficial tissue layers restricts the sensitivity and the depth to which fluorescence imaging can be used. Moreover, rapid photobleaching of fluorescent molecules limits their useful lifetime and prevents studies of prolonged duration. Therefore, we have attempted to develop new strategies that may solve some of the limitations of fluorescence imaging in living subjects.Raman spectroscopy can differentiate the spectral fingerprint of many molecules, resulting in very high multiplexing capabilities. Narrow spectral features are easily separated from the broadband autofluorescence, because Raman is a scattering phenomenon as opposed to absorption/emission in fluorescence, and Raman active molecules are more photostable compared with fluorophores, which are rapidly photobleached. Unfortunately, the precise mechanism for photobleaching is not well understood. However, it has been linked to a transition from the excited singlet state to the excited triplet state. Photobleaching is significantly reduced for single molecules adsorbed onto metal particles because of the rapid quenching of excited electrons by the metal surface...

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SERRS as a more sensitive technique for the detection of labelled oligonucleotides compared to fluorescence

Cited by 126 publications

References 12 publications

In vivo biodistribution and biological impact of injected carbon nanotubes using magnetic resonance techniques

In vivo biodistribution and biological impact of injected carbon nanotubes using magnetic resonance techniques

A Molecular Imaging Primer: Modalities, Imaging Agents, and Applications

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

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