SERS detection of radiation injury biomarkers in mouse serum

Abstract

In a large-scale radiological catastrophe, it is expected that hundreds and thousands of people could be exposed to radiation. A rapid method is required for triage of casualties to determine proper medical treatment. In this article, mice were exposed to different radiation doses and sera of mice were investigated by surface-enhanced Raman spectroscopy (SERS) and orthogonal projections to latent structure discriminant analysis (OPLS-DA) after total body irradiation (TBI). The results of the present study indicated that differences have widened over time. The different radiation groups showed a slight overlap at 24 h and 72 h but were completely distinct at the 10th day after TBI. The SERS spectrum between the normal group and the irradiated group showed a significant difference at 24 hours. The same trend was depicted in scatting score plots. Significant differences in Raman peaks were found, such as 744 and 1495 cm À1 corresponding to riboflavin and 593 and 1204 cm À1 corresponding to Ltryptophan. The lack of riboflavin and L-tryptophan will influence metabolism levels. Above all, these results bear potential in the development of label-free and rapid tools for on-site detection and screening of irradiation injuries.

Proteomic Profiling for Serum Biomarkers in Mice Exposed to Ionizing Radiation

“…To establish the mouse model in identical radiation conditions, mice were exposed to 0, 2, 5.5, 7, and 8 Gy and survival, body weight, and blood counts were assessed. Our published data 13 show that mice treated with 8 Gy die after 16 days, while only 4 of the 8 mice survive for 30 days following 7 Gy treatment. Mice treated with 0, 2, and 5.5 Gy all survived.…”

Accurate age-grading of field-aged mosquitoes reared under ambient conditions using surface-enhanced Raman spectroscopy and artificial neural networks

“…2, the spectral features of the field-aged F 2 female mosquitoes (field spectra) were similar but had more peaks identified when compared to SERS spectra of lab-reared female mosquitoes (lab spectra). Table 1 summarized the major characteristic peaks and their tentative assignments to either lab and/or field mosquitoes(Feng et al 2015, Kubryk et al 2016, Premasiri et al 2016, Li et al 2018, Shen et al 2018). Both of them contained peaks at 655, 729, 958, 1,092, and 1,328 cm –1 , with the most distinct peaks being the adenine-containing compounds at 729 cm –1 .…”

“…The PLS model demonstrated the capability of this approach to predict the age of field-aged mosquitoes between 0 and 22 days with R = Table 1. Tentative peak assignments of the SERS spectra of lab and field mosquitoes (adopted from Feng et al 2015, Kubryk et al 2016, Premasiri et al 2016, Li et al 2018, Shen et al 2018 Raman shift (cm −1 ) Lab Field Peak assignment 1,582 x x Guanine, adenine, tryptophan 1,447…”

Characterizing nanoplastics‐induced stress and its SERS fingerprint in an intestinal membrane model

“…[54] Although metabolomics is traditionally performed with mass spectroscopy, Raman spectroscopy, in particular surface enhanced Raman spectroscopy (SERS), has shown promise for the metabolic profiling of the cellular activity of bacterial cells and human cancer cells, [55][56][57][58][59][60] as well as for the detection of biomarkers in serum. [55,61,62] SERS is a vibrational spectroscopy that provides unique opportunities for signal amplification in aqueous media as water has low Raman cross-sections while the signal from Raman-active molecular vibrational modes in analytes can be enhanced by multiple orders of magnitude due to an electromagnetic enhancement effect in the vicinity of nanostructured noble metal surfaces. [63][64][65][66][67] This amplification of molecule-specific vibrational information facilitates a sensitive detection and identification of metabolites rapidly without the need for elaborate sample preparation or enrichment.…”