Sertoli–Leydig cell tumours of the ovary and testis: a CGH and FISH study

Verdorfer, Irmgard; Horst, Dale; Höllrigl, Alexandra; Rogatsch, Hermann; Mikuz, Gregor

doi:10.1007/s00428-006-0344-9

Cited by 10 publications

(6 citation statements)

References 18 publications

(18 reference statements)

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“…4 Imbalances and breakpoints of MLTC-1 according to mcb and CMA data were aligned to human homologous regions using Ensembl and the UCSC Genome Browser, as previously done [4]. The obtained data was compared to genetic changes known from human LCTs 5,6 , testicular germ cell tumors 7 and seminomas. [8][9][10][11]…”

Section: Methodsmentioning

confidence: 99%

First cytogenomic characterization of murine testis tumor cell line MLTC-1

Azawi

Kankel

Liehr

et al. 2022

Mol. exp. biol. med.

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The cell line MLTC-1 was established in 1982 as a transplantable Leydig cell tumor from a C57BL/6 mouse. The cell line has already been applied in >100 studies: still, the only information about its genetic content is given in the first description: MLTC-1 initially had a polyploid karyotype. Here, a molecular karyotyping and multicolor banding-based molecular cytogenetic study was done to provide the first chromosomal/ (molecular) cytogenetic characterization of MLTC-1. A hexaploid karyotype with 72 to 105 chromosomes was hereby characterized. Besides polyploidy, unbalanced two- and three-way translocations, dicentrics and one neocentric derivative were identified. Also, no Y-chromosome could be detected in this clearly male derived cell line. Overall, a completely unbalanced genome is present in MLTC-1 with ~20 regions being subject to copy number losses or gains. After translating these imbalances into the human genome in a standardized way, a 40% match of imbalances with human Leydig cell tumors was evident; however, about the same rate of concordance was detectable for human spermatocytic seminomas and non-seminomas as well as testicular germ cell tumor. Accordingly, MLTC-1 is better suited as an advanced testicular germ cell tumor model in general, rather than specifically for human Leydig cell tumors.

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Section: Methodsmentioning

confidence: 99%

First cytogenomic characterization of murine testis tumor cell line MLTC-1

Azawi

Kankel

Liehr

et al. 2022

Mol. exp. biol. med.

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“…It is important to remember that the lack of features characteristic of virilization and masculinization does not exclude Leydig cell ovarian neoplasm. In 25-30% of cases, there is no increase in the level of androgens [10].…”

Section: Introductionmentioning

confidence: 95%

Leydig cell ovarian tumor – clinical case description and literature review

Klimek¹,

Radosz²,

Lemm³

et al. 2020

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Leydig cell ovarian tumors constitute not only a medical problem for clinicians but also a social problemwhich is why women with symptoms of hirsutism relatively quickly contact physicians for medical consultation. Leydig cell ovarian tumor is a rare sex cord-gonadal stromal tumor which constitutes less than 0.5% of ovarian tumors. These cancers appear at all ages but the majority of the cases concern women in the perimenopause. In the majority of cases (70-85%), the growth is accompanied by androgen secretion, together with virilization and hirsutism. The presence of hormonally active ovarian cancers should be suspected in cases of rapidly growing symptoms of masculinization, especially when the level of free testosterone in the blood exceeds the upper limit for the given age more than three times. In diagnosing postmenopausal hyperandrogenism, it is necessary to take into account hormonally active ovarian tumors, as well as adrenal cancers. It is important to exclude other causes of hyperandrogenism, e.g. endocrinopathies (acromegaly or hypothyroidism), or iatrogenic and idiopathic factors. In order to make the diagnosis and implement the proper treatment method faster, an interdisciplinary team of physicians specializing in endocrinology, gynecology and oncology is crucial. This paper contains a study of two cases concerning Leydig cell ovarian tumors in women of postmenopausal age with symptoms of masculinization and hirsutism.

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“…Imbalances and breakpoints of I-10, based on the mcb and CMA data, were aligned to the human homologous regions using Ensembl and the UCSC Genome Browser, as previously described [5]. The data were compared to genetic changes known from human LCTs [6,7], seminomas, nonseminomas, and testicular germ cell tumors [8][9][10][11][12].…”

Section: Cell Line Work Up and Molecular (Cyto) Geneticsmentioning

confidence: 99%

“…The copy number changes associated with molecular subtypes of human LCTs and seminomas, according to previous studies[6][7][8][9][10][11][12], compared to the copy number variants (CNVs) in cell line I-10. Concordances with human CNVs are highlighted in bold.…”

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confidence: 99%

First Cytogenomic Characterization of the Murine Testicular Tumor Cell Line I-10

Azawi¹,

Barf²,

Liehr³

et al. 2022

OBM Genet

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After being established in 1967, the commercially available murine Leydig cell tumor line I-10 has been used in almost 50 published studies. I-10 has not been characterized, either at the chromosomal/ cytogenetic level or the genetic level, similar to many other murine tumor cell lines. In this study, we performed molecular karyotyping and multicolor banding-based molecular cytogenetics. A slightly hyperdiploid karyotype with 43 chromosomes was described. The main aberrations comprised several unbalanced translocations and three unusual rearrangements (two dicentric derivatives and one neocentric derivative). Nine regions showed copy number gains, and only five small chromosomal parts showed loss of copy numbers. A standardized translation of these imbalances in the human genome was performed, which showed a 63% overlap of the detected imbalances with testicular germ cell tumors, a 53% concordance with human spermatocytic seminomas and non-seminomas, and only a 36% overlap (approx.) of large copy number gains and losses were similar to the corresponding human Leydig cell tumors. However, no Y-chromosome was detected in this male-derived cell line. Overall, the I-10 cell line was found to be a testicular germ cell tumor model and cannot be treated as a model that is specific to human Leydig cell tumors. At best, it might be suited as a model for an early onset of Leydig cell tumors.

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Sertoli–Leydig cell tumours of the ovary and testis: a CGH and FISH study

Cited by 10 publications

References 18 publications

First cytogenomic characterization of murine testis tumor cell line MLTC-1

First cytogenomic characterization of murine testis tumor cell line MLTC-1

Leydig cell ovarian tumor – clinical case description and literature review

First Cytogenomic Characterization of the Murine Testicular Tumor Cell Line I-10

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