Serum Alkaline Phosphatase Fractions in Hepatobiliary and Bone Diseases

The nature of the serum alkaline phosphatases in liver diseases

“…Using polyacrylamide gel electrophoresis we found that in patients with obstructive liver disease the serum alkaline phosphatase showed a gross increase in the fast liver isoenzyme fraction with the appearance of several other isoenzyme fractions, the major contribution remaining at the origin. Our results are in agreement with those of other workers who employed similar techniques which included molecular sieving superimposed on the true electrophoretic mobility, for example, starch gel (Kowlessar, Pert, Haeffner, and Sleisenger, 1959;Moss, Campbell, Anagnostou-Karakas, and King, 1961;Chiandussi et al, 1962;Hodson et al, 1962;Taswell and Jeffers, 1963;and Hill and Sammons, 1967) and polyacrylamide gel (Jennings, Brocklehurst, and Hirst, 1970;Walker and Pollard, 1971). …”

Alkaline Phosphatases in Biology and Medicine

“…Making it even more complex, it has been known for quite some time [ 124] that the AP in bile differs from AP derived from the liver cells. The biliary AP was thought to consist from a protein-phospholipid complex [125] which was more heat-stable than the liver AP [56], Improvement in analytic procedures re sulted in a substantial elucidation of the pro cess of regurgitation of AP from the liver [47.…”

The nature of the alkaline phosphatases of bile

“…However, more recently several workers, using a variety of techniques, have shown differences between the main AP isoenzymes present in serum and bile. Starch gel electrophoretic studies indicate that the main alkaline phosphatase isoenzyme present in the bile differs from that present either in the serum (Pope and Cooperband, 1966) or in the liver tissue (Chiondussi, Greene, and Sherlock, 1962;Hill and Sammons, 1967).…”