Serum alkaline phosphatase polymorphism in pigs¹

Abstract

Summary Genetic variants of serum alkaline phosphatase were studied by the method of starch gel electrophoresis in the Zlotnicka Pstra breed of pigs. Two regions of alkaline phosphatase migration were observed. A single fraction in region I and four different phenotypes: AB, B, BC and BD in region II, were found. For AB, B and BC phenotypes the genetic control by three alleles AkpA, AkpB and AkpC in suggested. The observed segregation ratios in some cases deviated significantly from the expected ones.

Serum and tissue alkaline phosphatases in pigs*

“…Up till now, the heterogeneity of serum AP in pigs has been interpreted as being .due to genetic polymorphism (Batra & Khanna, 1981;Przytulski et al, 1982). However, differences between observed and expected frequencies of phenotypes were reported by Kierek-Jaszczuk et al (1979). Now we have found that the properties and tissue origin for serum APs of the A ' , A and C zones differ from those of the B zone (Table 1) and hence the serum APs must be controlled by at least two loci.…”

“…U p till now, it was unknown which kind of system regulates the appearance of pig AP. In pig serum the majority of mature animals have neither A nor C zones (Kierek-Jaszczuk et al 1979 and only for a few animals either the A or the C zone could be observed together with the B zone. Only in developing piglets, all serum APs occurred simultanously (Kierek-Jaszczuk & Zurkowski, 1980).…”

“…Native and pretreated samples of serum and tissue extracts were analysed by horizontal starch gel electrophoresis (STAGE) as described by Kierek-Jaszczuk et al (1979) and by vertical polyacrylamide gel electrophoresis (PAGE). PAGE was performed in slabs (220 X 115 X 2 mm) between glass plates which were cooled at 5 "C (+ 1 "C).…”

“…In PAA as well as starch gels the enzyme was visualized as described by Kierek-Jaszczuk et al (1979) using a staining mixture with l-naphthyl phosphate and Fast Blue BB salt. The PAA gels were incubated at 37 "C only 30 min and then the staining mixture was replaced by a new one in which the gels were left overnight at 4 "C.…”

“…The A P variants observed later on were suggested to be controlled by 5 (Dinklage, 1970) or 3 (Verhorst, 1973) codominant alleles of an autoso-ma1 locus. However, assuming this mode of inheritance, an excess of animals with the A P B phenotype and no animals with the AP A C and C phenotypes were observed (Kierek-Jaszczuk et al, 1979).…”