Serum Amylase and Serum Lipase Levels in Man after Administration of Secretin and Pancreozymin

Burton, P.; Hammond, Elizabeth; Harper, A. A.; Howat, Henry T.; Scott, J. E.; Varley, H.

doi:10.1136/gut.1.2.125

Cited by 65 publications

(22 citation statements)

References 13 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this table the incidence of duodenal ulcer (Table 7). (Burton et al, 1960b reatitis is a reduced maxirmal bicarbonate concentration and reduced bicarbonate output following a standard submaximal dose of secretin and in somes cases a reduced enzyme output following pancreozymin is obtained (Fig. 3).…”

Section: Aetiologymentioning

confidence: 97%

“…In the earlier stages significant elevations of serum amylase and lipase may be found during exacerbations of pain or after stimulating the pancreas with secretin and pancreozymin (Burton et al, 1960b). The evocative test is best combined with an oral glucose tolerance test; the combined test indicated pancreatic involvement in fortythree of forty-nine patients (87%) who had chronic pancreatitis and in a similar percentage of patients with primary cancer of the pancreas.…”

Section: Pancreatic Insufficiencymentioning

confidence: 99%

See 1 more Smart Citation

Chronic pancreatitis: medical aspects

Howat

1968

Postgraduate Medical Journal

Self Cite

View full text Add to dashboard Cite

Section: Aetiologymentioning

confidence: 97%

Section: Pancreatic Insufficiencymentioning

confidence: 99%

Chronic pancreatitis: medical aspects

Howat

1968

Postgraduate Medical Journal

Self Cite

View full text Add to dashboard Cite

“…Pilocarpine (1) and other secretory stimulants (2)(3)(4) are known to increase the enzyme content of the blood and lymph. Numerous other investigations have indicated that these enzymes originate from the pancreas and enter the blood to a large extent by means of the lymphatic pathway (5)(6)(7)(8).…”

Section: Effect Of Pilocarpine and Pancreatectomy On Amylase And Lipasementioning

confidence: 99%

Effect of Pilocarpine and Pancreatectomy on Amylase and Lipase Levels of Serum and Hepatic Lymph

Singh

Appert

1969

Experimental Biology and Medicine

View full text Add to dashboard Cite

Pilocarpine (1) and other secretory stimulants (2-4) are known to increase the enzyme content of the blood and lymph. Numerous other investigations have indicated that these enzymes originate from the pancreas and enter the blood to a large extent by means of the lymphatic pathway (5-8). Recently, it has been shown that rat liver synthesized amylase in isolated perfusion experiments (9, 10). Relatively little information, however, is available on the contribution of hepatic lymph to the increased enzyme levels observed in the thoracic duct lymph after the administration of various secretory stimulants. In this series of experiments, enzyme levels were studied in normal dogs as well as in pancreatectomized dogs in order to evaluate the effect of pilocarpine on the production of serum enzymes by the liver as well as the pancreas. In addition, the enzyme levels of the hepatic and portal blood were studied in order to determine the relative importance of the venous versus the lymphatic pathways.Materials and Methods. Two series of experiments were performed. Mongrel dogs of either sex, weighing 15-20 kg, were used in these experiments. Six experiments were done in the first control series. The animals were subjected to laparotomy under pentobarbital anesthesia and controlled endo tracheal ven tilation. Lymphatic vessels were identified in the hilum of the liver after injecting Evan's blue dye subcapsularly in the liver. The efferent lymphatics from the hepatic lymph node were stripped of the surrounding tissue, and the largest one was incised. A polyethylene tube was inserted toward the liver, being held in place by ligatures.The remaining efferent lymphatics from the hepatic lymph node, as well as those * Supported by Grants AM-02723, HE-04182 from the National Institutes of Health. coming towards it from the direction of the pancreas and duodenum, were ligated ( 11). The right side of the thorax was then opened along the ninth intercostal space. A polyethylene tube was introduced into the inferior vena cava and passed down into the hepatic vein. This tube was available for sampling of hepatic venous blood.Cannulation for sampling of the portal vein was accomplished by passing a similar tube through the superior mesenteric vein into the portal vein. Peripheral blood was sampled from the femoral vein.Two control samples of heparinized lymph and blood were taken simultaneously at 20-min intervals from each of the catheters. Pilocarpine (20 mg diluted in 200 ml of normal saline) solution was administered intravenously by drip over a period of 40 min. Four samples of lymph and blood, each simultaneously at 20-min intervals, were taken after pilocarpine infusion (Table I A and B).The lymph samples were collected continuously throughout each 20-min interval.In the second series of four experiments, hepatic lymphatic cannulation was done immediately after subjecting the animal to ,total pancreatectomy. Three samples of hepatic lymph and peripheral venous blood, each obtained at 15-min intervals, were taken simultaneousl...

show abstract

“…Another role for semm amylase assay concerns the response o f the pancreas to stimulation by the entero-hormones secretin and cholecystokinin (CCK) (33). Normal individuals show little change.…”

Section: Serum Amylasementioning

confidence: 99%

Amylase, Isoamylase and Macroamylase

Goldberg¹,

Spooner²

1975

Digestion

View full text Add to dashboard Cite

Hyperamylasaemia has long been regarded as pathognomonic of acute pancreatitis. However, recent work has revealed a number of conditions where a gross elevation may be an incidental finding, notably diabetic ketoacidosis. The recent discovery of ‘macroamylase’, a high molecular weight amylase-protein complex capable of producing hyperamylasaemia with low urine amylase, has further complicated diagnosis and has led to the introduction of the ratio of amylase clearance to creatinine clearance as a diagnostic aid. Serum amylase may be resolved, by most electrophoretic media, into bands which correspond to those obtained when pancreatic homogenates or saliva are electrophoresed. The initial promise of this technique has not been realised at the routine diagnostic level. Duodenal juice amylase has been the classical enzyme used in assessing exocrine pancreatic function and although it is still of value it is being amplified by other enzyme tests.

show abstract

Serum Amylase and Serum Lipase Levels in Man after Administration of Secretin and Pancreozymin

Cited by 65 publications

References 13 publications

Chronic pancreatitis: medical aspects

Chronic pancreatitis: medical aspects

Effect of Pilocarpine and Pancreatectomy on Amylase and Lipase Levels of Serum and Hepatic Lymph

Amylase, Isoamylase and Macroamylase

Contact Info

Product

Resources

About