Serum Amyloid A: Expression Throughout Human Ovarian Folliculogenesis and Levels in Follicular Fluid of Women Undergoing Controlled Ovarian Stimulation

Urieli-Shoval, Simcha; Finci-Yeheskel, Z; Eldar, Ido; Linke, Reinhold P.; Levin, Mark; Prus, Diana; Haimov‐Kochman, Ronit

doi:10.1210/jc.2012-1801

Cited by 18 publications

(16 citation statements)

References 39 publications

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“…They found a strong correlation between serum and FF levels and also reported a significant association between BMI and serum AA protein concentrations, that is higher levels associated with increased BMI. Besides obesity, controlled ovarian hyperstimulation, which induces a pro-inflammatory state [27], was indicated as second reason of elevated levels of serum AA protein [23]. In our study, although not statistically significant, both the levels of FF and serum AA protein levels of nonobese PCOS group revealed higher levels than control group.…”

Section: Discussioncontrasting

confidence: 60%

“…Besides TNF-α, several other cytokines, like IL-6, was found to be secreted from adipose tissue [22]. Serum AA protein is another acute phase protein which is thought to be more specific to PCOS-related inflammation [23]. Serum AA protein is produced by the liver in response to inflammatory stimuli [24].…”

Section: Discussionmentioning

confidence: 99%

“…Urieli-Shoval et al [23] studied follicular fluid and serum levels of AA protein in IVF patients. They found a strong correlation between serum and FF levels and also reported a significant association between BMI and serum AA protein concentrations, that is higher levels associated with increased BMI.…”

Section: Discussionmentioning

confidence: 99%

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The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome

Tımur

Yimaz

Kahyaoğlu

et al. 2015

J Assist Reprod Genet

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Purpose In this study, we aimed to investigate serum and follicular fluid amyloid A protein levels in non-obese nonhyperandrogenic patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF) and IVF outcome. Methods A total of 81 patients undergoing IVF treatment, 41 patients diagnosed as PCOS according to the Rotterdam criteria (group I) and 40 patients with the etiology of male factor infertility (group II), were included in the study. On the day of oocyte pickup, serum and follicular fluid samples were collected from all patients. Results Serum E2 level on the day of hCG (2849.93±541.54 vs. 2494.28±712.98) and total number of retrieved oocytes (13.73±3.57 vs. 10.53±4.07) were significantly higher in group I when compared to group II (p<0.05). However, number of mature oocytes, fertilization rate, and clinical pregnancy rate did not differ (p>0.05). No significant difference was found between two groups regarding the serum and follicular fluid amyloid A protein levels on the day of oocyte retrieval (p>0.05).

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Section: Discussioncontrasting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

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The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome

Tımur

Yimaz

Kahyaoğlu

et al. 2015

J Assist Reprod Genet

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“…In the last years, several works have been performed to determine the protein composition of the human follicular fluid. The majority of such researches were mainly limited to the identification and description of the fluid components, without providing significant insights into the roles that these proteins may exert in follicle physiology and in IVF outcome (Refs 3, 4, 5, 6, 7, 8). Hence, a more deep comprehension of molecular processes would help clinicians to increase success in ART procedures also by treating specific categories of patients with a personalised approach.…”

Section: Introductionmentioning

confidence: 99%

Protein pathways working in human follicular fluid: the future for tailored IVF?

Bianchi

Gagliardi

Landi

et al. 2016

Expert Rev. Mol. Med.

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The human follicular fluid (HFF) contains molecules and proteins that may affect follicle growth, oocyte maturation and competence acquiring. Despite the numerous studies, an integrated broad overview on biomolecular and patho/ physiological processes that are proved or supposed to take place in HFF during folliculogenesis and oocyte development is still missing. In this review we report, for the first time, all the proteins unambiguously detected in HFF and, applying DAVID (Database for Annotation, Visualization and Integrated Discovery) and MetaCore bioinformatic resources, we shed new lights on their functional correlation, delineating protein patterns and pathways with reasonable potentialities for oocyte quality estimation in in vitro fertilisation (IVF) programs. Performing a rigorous PubMed search, we redacted a list of 617 unique proteins unambiguously-annotated as HFF components. Their functional processing suggested the occurrence in HFF of a tight and highly dynamic functional-network, which is balanced by specific effectors, primarily involved in extracellular matrix degradation and remodelling, inflammation and coagulation. Metalloproteinases, thrombin and vitamin-Dreceptor/retinoid-X-receptor-alpha resulted as the main key factors in the nets and their differential activity may be indicative of ovarian health and oocyte quality. Despite future accurate clinical investigations are absolutely needed, the present analysis may provide a starting point for more accurate oocyte quality estimation and for defining personalised therapies in reproductive medicine.

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“…where higher levels of ALS were found in good prognosis patients undergoing IVF, such as normal body mass index, brief controlled ovarian stimulation, AMH level between 1 and 3 ng/ml, and diagnosis of male or unexplained factor. Another interesting finding was described by Urieli-Shoval et al [48]. Levels of serum amyloid A(SAA), a different type of amyloid protein related to inflammatory processes, such as tissue injury, infection and neoplasia, were measured in follicular fluid aspirates and blood samples from infertile women undergoing controlled ovarian stimulation cycles.…”

Section: Discussionmentioning

confidence: 82%

Identificação e caracterização de substância amiloide em oócitos murinos e humanos e embriões de camundongos

Pimentel¹

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Introduction: Previous studies with budding yeasts evidenced an important role of amyloid substances (AS) in the regulation of gametogenesis, questioning its potential as a marker of gametic quality. However, to date, no study has evaluated the presence of AS in mammalian oocytes and embryos. Objectives: To describe the presence, to characterize the distribution pattern and to compare AS levels in mature oocytes and preimplantation embryos using murine model. Identify, characterize the distribution pattern and quantify AS in human oocytes at different stages of development (GV-germinal vesicle, MImetaphase I and MII-metaphase II) and establish the correlation between oocytes AS levels and clinical characteristics of patients undergoing control ovarian stimulation (COE) for in vitro fertilization (IVF). Methods: An experimental prospective pilot study was carried out using 20 mature oocytes and 200 preimplantation mouse embryos (1-cell, 2-cell, 4-cell, 8cell and blastocysts) and 46 immature and in vitro mature human oocytes donated from 11 consenting patients submitted to COE for IVF. Frozen MII oocytes and mouse preimplantation embryos, immediately after thawing, were fixed for immunostaining for visualization of AS by confocal microscopy and by transmission electron microscopy. Immature (GV and MI) and in vitro mature (GV Arrested, MI, MI Arrested and MII) human oocytes were also fixed and immunostained for AS evaluation, using the same antibody, by confocal microscopy. Fluorescence intensity from immunofluorescent staining and data from confocal microscopy were quantified using ImageJ program. Results: In all samples (mice and humans), immunostaining for AS appears throughout the zona pellucida, as well as in the cytoplasm and nucleus of oocytes, polar bodies, and preimplantation embryos. In mouse, 2-cell embryos exhibited higher levels of AS (69000187,4 SD 6733098,1 a.u.) when compared to the other stages of development (<0.0001). Electron microscopy confirmed the presence of AS in mouse oocytes and preimplantation embryos. In humans, fresh GV stage oocytes exhibited higher levels of AS (4164.7 SD 1573.5 a.u.) when compared to MI and MII oocytes (= 0.008). A negative association was found between levels of AS and patient body mass index (BMI) (-0.54; = 0.0007), number of days of control ovarian stimulation (-0.44; = 0.002); (-0.34, = 0.002), dose of gonadotropin used, time between oocyte retrieval and fixation (-0.33; = 0.02) and time after the hCG trigger (-0.33; = 0.02). Significantly higher levels of AS were found in patients with AMH between 1 and 3 ng/ml, compared to <1 ng/ml (4592.

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Serum Amyloid A: Expression Throughout Human Ovarian Folliculogenesis and Levels in Follicular Fluid of Women Undergoing Controlled Ovarian Stimulation

Abstract: SAA is locally produced in ovarian developing follicles and is a constituent of follicular fluids, suggesting its role within the follicular environment. Elevated follicular SAA levels are associated with decreased pregnancy rate and may signify lower reproductive performance.

Cited by 18 publications

References 39 publications

The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome

The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome

Protein pathways working in human follicular fluid: the future for tailored IVF?

Identificação e caracterização de substância amiloide em oócitos murinos e humanos e embriões de camundongos

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