Serum amyloid A in the serum and milk of ewes with mastitis induced experimentally with<i>Staphylococcus</i>epidermidis

Winter, P.; Fuchs, Klemens; Walshe, Kieran; Colditz, Ian G.

doi:10.1136/vr.152.18.558

Cited by 26 publications

(21 citation statements)

References 24 publications

(30 reference statements)

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“…The findings showed that acute phase reaction developed in cows with subclinical mastitis and confirmed earlier observations reported by other authors (Winter et al 2003, Grönlund et al 2005, Eckersall et al 2006, Akerstedt et al 2007, Hiss et al 2007, Szczubiał et al 2008, Pyörälä et al 2011. Acute phase proteins are mainly produced in the liver, yet it was demonstrated that both SAA , Weber et al 2006 and Cp (Jaeger et al 1991, Cerveza et al 2000, Donley et al 2002 could also be produced in the udder.…”

Section: Discussionsupporting

confidence: 81%

Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens

Szczubiał¹,

Dąbrowski²,

Kankofer³

et al. 2012

Polish Journal of Veterinary Sciences

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The aim of the present study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with subclinical mastitis caused by different pathogens. Eighty-four milk samples from cows with subclinical mastitis and fourteen milk samples from healthy cows were examined. SAA concentration was determined using the commercial ELISA kit (Tridelta Development Ltd., Greystones, Wicklow, Ireland). Cp activity was assessed spectrophotometrically, using the Rice method. The results reveal that the concentration of SAA (with exception of CNS) and activity of Cp in cow milk can be regarded as markers of subclinical mastitis, irrespective of the microorganism inducing the disease. In conclusion, measurement of SAA and Cp in milk samples could be a useful method in diagnosing subclinical mastitis in cows, but the method should be adapted for field use.

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Section: Discussionsupporting

confidence: 81%

Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens

Szczubiał¹,

Dąbrowski²,

Kankofer³

et al. 2012

Polish Journal of Veterinary Sciences

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“…In a previous study, we found elevated levels of both haptoglobin and SAA in milk from udder quarters with sub-clinical mastitis 21 to 35 days after experimental infection with Staphylococcus aureus, but only SAA was significantly different from pre-infection levels [6]. Winter et al [22] also described elevated levels of SAA during sub-clinical experimentally induced Staphylococcus epidermidis mastitis in ewes. Several studies have also stated that healthy cows have very low, or undetectable, serum levels of SAA and haptoglobin [1][2][3]6], and this is also the case in milk from healthy quarters [3,6].…”

Section: Introductionmentioning

confidence: 90%

Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis

2005

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-New tools are needed to detect chronic sub-clinical mastitis, especially in automatic milking systems. Haptoglobin and serum amyloid A (SAA) are the two most sensitive bovine acute phase proteins, and their concentrations increase in milk from cows with clinical mastitis and in milk from cows with experimentally induced chronic sub-clinical Staphylococcus aureus mastitis. The aim of this study was to further evaluate the potential for haptoglobin and SAA in milk as indicators of chronic sub-clinical mastitis. Quarter milk samples were collected from 41 cows with a mean composite milk somatic cell count (CSCC) above 300 000 cells/mL during at least two months prior to sampling. Quarter milk samples were also taken from eleven cows with a mean CSCC below 80 000 cells/mL during at least two previous months. These samples were analysed for haptoglobin, SAA, adenosine triphosphate (ATP) activity and bacterial growth. The samples were grouped according to their ATP, haptoglobin and SAA status. ATP+ samples had ATP > 2 × 10 -10 mol/mL, Hp+ and SAA+ samples had detectable levels of haptoglobin (≥ 0.3 mg/L) and SAA (≥ 0.9 mg/L), respectively. In udder quarter samples from healthy cows, 42 out of 44 samples belonged to the ATPHp-SAA-group. Among cows with chronic sub-clinical mastitis, the ATP+Hp+SAA+ group contained 66 out of 164 samples while 44 samples belonged to the ATP+Hp-SAA-group. Detectable levels of haptoglobin and SAA were found in 92 and 80 samples, respectively. Growth of udder pathogens was detected in 28 samples and Staphylococcus aureus was the most common bacteria. In conclusion, haptoglobin and SAA concentrations below the detection limit were considered as good indicators of healthy udder quarters. A substantial variation in haptoglobin and SAA concentrations in milk was observed in udder quarters with chronic sub-clinical mastitis. serum amyloid A / haptoglobin / chronic / sub-clinical / mastitis

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“…Whereas SAA levels in milk may be useful for the diagnosis on subclinical mastitis in individual ewes further studies are needed to determine its usefulness from bulk milk (Winter et al, 2006). Interestingly, opposite to bovine, the changes in the concentration of SAA in ewes with mastitis experimentally induced by Staphylococcus epidermidis are observed earlier in serum than in milk (Winter et al, 2003). In goats the studies are rather limited than in sheep.…”

Section: Apps In Small Ruminantsmentioning

confidence: 87%

Acute Phase Proteins as Biomarkers in Animal Health and Welfare

Gómez-Laguna¹,

Salguero²,

Pallarés³

et al. 2011

Acute Phase Proteins as Early Non-Specific Biomarkers of Human and Veterinary Diseases

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Serum amyloid A in the serum and milk of ewes with mastitis induced experimentally withStaphylococcusepidermidis

Cited by 26 publications

References 24 publications

Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens

Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens

Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis

Acute Phase Proteins as Biomarkers in Animal Health and Welfare

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