Serum antibodies to antigens derived from a saline extract of : Correlation with resistance to experimental bovine pneumonic pasteurellosis

Confer, Anthony W.; Lessley, Bruce A.; Panciera, Roger J.; Fulton, Robert W.; Kreps, J.A.

doi:10.1016/0165-2427(85)90052-2

Cited by 47 publications

(12 citation statements)

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“…These include live M. haemolytica (2,6,7,9,11,19,21,33,50), killed M. haemolytica cells (10,20,22,23,52), components or fractions of M. haemolytica cells (17,24,27,28,35,49,(53)(54)(55), and commercial vaccines (57,58). While immunity to M. haemolytica appears to require anti-LKT and antisurface antigen antibodies (55), partial protection could be afforded to cattle experimentally vaccinated with M. haemolytica preparations that did not contain LKT (21,40).…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of activity of antisurface antibodies and the specific surface antigens involved in anti-M. haemolytica immunity are not known; however, complementmediated bacterial lysis and bacterial phagocytosis and killing are thought to be important in defense against M. haemolytica infection (45). Complement-mediated bactericidal activity against M. haemolytica and phagocytosis of M. haemolytica by bovine neutrophils has been demonstrated with bovine immune serum (12,17,40,46).Little is known about the specific surface antigens that are important in stimulating host immunity to M. haemolytica. However, several studies point toward the importance of outer membrane proteins (OMPs).…”

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Characterization of Immunodominant and Potentially Protective Epitopes ofMannheimia haemolyticaSerotype 1 Outer Membrane Lipoprotein PlpE

2004

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Mannheimia haemolytica serotype 1 (S1) is the most common bacterial isolate found in shipping fever pneumonia in beef cattle. Currently used vaccines against M. haemolytica do not provide complete protection against the disease. Research with M. haemolytica outer membrane proteins (OMPs) has shown that antibodies to one particular OMP from S1, PlpE, may be important in immunity. In a recently published work, members of our laboratory showed that recombinant PlpE (rPlpE) is highly immunogenic when injected subcutaneously into cattle and that the acquired immunity markedly enhanced resistance to experimental challenge (A. W. Confer, S. Ayalew, R. J. Panciera, M. Montelongo, L. C. Whitworth, and J. D. Hammer, Vaccine 21:2821-2829, 2003). The objective of this work was to identify epitopes of PlpE that are responsible for inducing the immune response. Western blot analysis of a series of rPlpE with nested deletions on both termini with bovine anti-PlpE hyperimmune sera showed that the immunodominant region is located close to the N terminus of PlpE. Fine epitope mapping, in which an array of overlapping 13-mer synthetic peptides attached to a derivatized cellulose membrane was probed with various affinity-purified anti-PlpE antibodies, identified eight highly reactive regions, of which region 2 (R2) was identified as the specific epitope. The R2 region is comprised of eight imperfect repeats of a hexapeptide (QAQNAP) and is located between residues 26 and 76. Complementmediated bactericidal activity of affinity-purified anti-PlpE bovine antibodies confirmed that antibodies directed against the R2 region are effective in killing M. haemolytica.Bovine respiratory disease arises from the interaction of numerous contributing factors, including physical stresses associated with weaning, shipment, inclement weather, and overcrowding coupled with viral and bacterial infections (8,31,32,59,63). The result in severe cases is colonization of the lungs with pathogenic bacteria resulting in severe pneumonia. Pasteurella multocida, Haemophilus somnus, and Mannheimia (formerly Pasteurella) haemolytica are associated with bovine pneumonia. However, M. haemolytica serotype 1 (S1) is by far the most important bacterial pathogen in the development of the often-fatal fibrinous pleuropneumonia in beef cattle known as pneumonic pasteurellosis or shipping fever (31, 32).Immunity against M. haemolytica is thought to be primarily through production of serum antibodies that neutralize the secreted leukotoxin (LKT) and antibodies against surface antigens (45). The mechanism of activity of antisurface antibodies and the specific surface antigens involved in anti-M. haemolytica immunity are not known; however, complementmediated bacterial lysis and bacterial phagocytosis and killing are thought to be important in defense against M. haemolytica infection (45). Complement-mediated bactericidal activity against M. haemolytica and phagocytosis of M. haemolytica by bovine neutrophils has been demonstrated with bovine immune serum (12,17,40,46).Litt...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Characterization of Immunodominant and Potentially Protective Epitopes ofMannheimia haemolyticaSerotype 1 Outer Membrane Lipoprotein PlpE

2004

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“…The porins are also able to induce in£ammatory processes [31] as shown for LAPs [18]. Furthermore, among the OMPs, the LPS-associated porins may represent the dominant antigenic factor which stimulates the production of protective antibodies [32,33]. The protective activity of OMPs used as antigen, has been demonstrated experimentally by using Neisseria [34] and Haemophilus [35] OMPs.…”

Section: Introductionmentioning

confidence: 99%

Effect of Pasteurella haemolytica outer membrane proteins on bovine neutrophils

Iovane

Galdiero

Vitiello

et al. 2006

FEMS Immunology &Amp; Medical Microbiology

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The major outer membrane proteins (OMPs) isolated from Pasteurella haemolytica induce alterations of the biological activity of bovine polymorphonuclear leukocytes (PMNs). A dose‐dependent reduction of the capacity of adherence to nylon wool in vitro was observed. OMPs also acted as chemotaxins at concentrations between 5 and 20 μg/ml. Concentrations lower than 5 μg/ml did not give considerable results. Preincubation with 5 μg/ml of OMPs led to modifications in the values of the phagocytic index and of intracellular killing, which were found to be diminished with respect to controls.

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“…27 Levamisole hydrochloride effects on humoral and cellmediated immune response in several diseases have been examined. 28 Levamisole described as antiproliferative and affect both adhesion and MHC class I molecule expression. 29 Endothelial cells exposed to levamisole exhibited considerable vacuolization and increased rates of apoptotic cell death as apparent by condensation of chromatin and the presence of apoptotic vesicles when compared to their individual control cells without added levamisole.…”

Section: Citationmentioning

confidence: 99%

Effect of Levamisol and Vitamine E/ Selenium on Bovine Cellular and Humeral Immunity after Bovine Viral Diarrhea Vaccination

Sayed-Ahmed¹

2015

JDVAR

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Serum antibodies to antigens derived from a saline extract of : Correlation with resistance to experimental bovine pneumonic pasteurellosis

Cited by 47 publications

References 5 publications

Characterization of Immunodominant and Potentially Protective Epitopes ofMannheimia haemolyticaSerotype 1 Outer Membrane Lipoprotein PlpE

Characterization of Immunodominant and Potentially Protective Epitopes ofMannheimia haemolyticaSerotype 1 Outer Membrane Lipoprotein PlpE

Effect of Pasteurella haemolytica outer membrane proteins on bovine neutrophils

Effect of Levamisol and Vitamine E/ Selenium on Bovine Cellular and Humeral Immunity after Bovine Viral Diarrhea Vaccination

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