Serum antibodies to <i>klebsiella</i> capsular polysaccharides in ankylosing spondylitis

Sahly, Hany; Podschun, Rainer; Sass, Rainer; Bröker, Barbara M.; Kekow, Jörn; Gross, Wolfgang L.; Ullmann, U.

doi:10.1002/art.1780370521

Cited by 28 publications

(15 citation statements)

References 26 publications

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“…However, if the organism is grown on the same media supplemented with pullulan (0.3% w/v) as the carbon source the degree of antibody binding in AS patients is greatly increased. This increase also shows that the capsule is highly antigenic and is one of the predominant antigens recognised by AS patients, an observation also made by other workers [14]. In the control experiment, where P mirabilis is grown in the same conditions, with and without pullulan, antibody elevations are only seen in RA patients not AS or healthy controls.…”

Section: Discussionsupporting

confidence: 83%

Molecular mimicry and ankylosing spondylitis: possible role of a novel sequence in pullulanase of Klebsiella pneumoniae

et al. 1995

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Molecular mimicry has been shown between two sequences of Kiebsieilapneumoniae puID secretion protein (DRDE) with HLA-B27 (DRED) and pulA (pullulanase) enzyme (Gly-XPro) with types I, lII and IV collagen respectively. IgG antibody levels in AS patients were elevated against 16mer synthetic peptides of HLA-B27 and pulD by enzyme linked immunosorbent assay (ELISA) compared to controls (P < 0.001). ELISA assays against K. pneumoniae grown in the absence and presence of pullulan demonstrated significant levels of IgA antibody in AS patients compared to controls (P < 0.001). Increased IgA and lgG antibody levels to pulA and types I and IV collagen were observed in AS patients compared to controls (P < 0.001). These observations could be relevant in the sequence of molecular events in AS.

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Section: Discussionsupporting

confidence: 83%

Molecular mimicry and ankylosing spondylitis: possible role of a novel sequence in pullulanase of Klebsiella pneumoniae

et al. 1995

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“…There is strong evidence for the involvement of humoral and cell-mediated immunity in the pathogenesis of AS, though investigations on abnormalities in immune cell numbers in peripheral blood [15][16][17], cytokine production [7,8,18] and occurrence of certain antibodies to different microbes [2,19,20] show conflicting results. It should be considered that there is a wide diversity of presenting symptoms in AS, and clinical expression varies a lot [21].…”

Section: Discussionmentioning

confidence: 99%

Stimulation of whole blood cultures in patients with ankylosing spondylitis by a mitogen derived fromMycoplasma arthritidis (MAS) and other mitogens

et al. 1997

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In this study we compared cytokine production and cell proliferation of immunocompetent cells derived from patients with ankylosing spondylitis (AS) to those from healthy blood donors using a whole blood assay. To this end, blood cell cultures were stimulated with the superantigens MAS (Mycoplasma arthritidis supernatant) and staphylococcal enterotoxin B (SEB) and the plant lectins phytohaemagglutinin (PHA) and concanavalin A (Con A). The number of white blood cells (WBC) and lymphocyte subsets were also determined. Cell proliferation and levels of interferon-gamma (IFN-gamma), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) were measured after stimulation with the different mitogens. An ELISA test was used to analyse supernatant cytokine levels. Individuals with AS showed significantly lower IFN-gamma concentrations and markedly lower cell proliferation rates with all tested mitogens than healthy controls, while there was no significant difference in IL-6 synthesis. IL-1 beta levels were slightly impaired in the patient group, but only blood cell cultures stimulates with MAS showed a statistical significance. Furthermore, there was a significant elevation of leucocytes and lymphocytes in patients with AS resulting in higher numbers of CD4-positive cells, which implies a higher CD4:CD8 cell ratio. CD19- and CD8-positive cells were not significantly distinct compared to healthy controls. This deviation in cytokine levels and cell proliferation points to a suppression of T lymphocytes. A disturbed T-lymphocyte function may play a part in the pathogenesis of AS.

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“…The parent K2 and K21a encapsulated strains were previously described (26). Spontaneous noncapsulated variants of serotypes K2, K21a, K26, K50, and K36 were obtained from nonmucoid segments of colonies of capsulated parent strains as described elsewhere (22,37). Likewise, a revertant capsulated K21a variant was obtained from mucoid segments in colonies of the noncapsulated K21a strain.…”

Section: Methodsmentioning

confidence: 99%

Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

Podschun²,

et al. 2000

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The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistant Klebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization of Klebsiella.Klebsiella pneumoniae is an opportunistic pathogen involved in outbreaks of nosocomial infections, such as bacteremia and sepsis, mainly in immunocompromised individuals (36). Due to the emergence of multidrug resistance among Klebsiella strains, the search for new approaches for the prevention or treatment of Klebsiella infections is now under intensive investigation (34). To be successful, these efforts will require a better understanding of the infectious process.Multiple Klebsiella components (e.g., fimbriae, siderophores, O antigens, and capsular antigens) have been considered to be potential virulence factors (34). Of these factors, capsular antigens are probably considered the major determinants of pathogenicity (4,9,17,21). As a consequence, new therapeutic approaches have been targeted against the capsule. A polysaccharide-based vaccine has been tested (5). Based on the structural variability of its capsular polysaccharides (CPS), Klebsiella has been classified into 77 serotypes (27), which differ markedly in pathogenicity potential and epidemiological relevance (3,28,29,35). Epidemiological findings showed that over 70% of all cases of Klebsiella bacteremia were caused by only 25 of the 77 different serotypes (6).

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Serum antibodies to klebsiella capsular polysaccharides in ankylosing spondylitis

Cited by 28 publications

References 26 publications

Molecular mimicry and ankylosing spondylitis: possible role of a novel sequence in pullulanase of Klebsiella pneumoniae

Molecular mimicry and ankylosing spondylitis: possible role of a novel sequence in pullulanase of Klebsiella pneumoniae

Stimulation of whole blood cultures in patients with ankylosing spondylitis by a mitogen derived fromMycoplasma arthritidis (MAS) and other mitogens

Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

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