Serum antibody responses to <i>Wolbachia</i> surface protein in patients with human lymphatic filariasis

Shiny, C.; Krushna, N.S.A.; Archana, Bairavasundaram; Farzana, Begum; Narayanan, R. B.

doi:10.1111/j.1348-0421.2009.00172.x

Cited by 10 publications

(12 citation statements)

References 30 publications

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“…(2009) recently reported a 54‐year‐old man from Haiti who, although initially microfilaraemic (with 100 microfilariae/ml blood) and seropositive for antifilarial IgG4, became amicrofilaraemic (and persistently seronegative for antifilarial IgG4) after treatment with diethylcarbamazine but developed lymphoedema in one leg approximately 1 year later. In this patient, titres of antifilarial IgG1 began to increase after the development of the lymphoedema, peaking after a further 2 years (Shiny et al ., 2009). Similar observations were made by Kurniawan et al .…”

Section: Correlation Of Igg4 Levels With the Absence Of Pathology In mentioning

confidence: 86%

Induction of immunoglobulin G4 in human filariasis: an indicator of immunoregulation

Adjobimey¹,

Hoerauf²

2010

Annals of Tropical Medicine & Parasitology

144

148

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Filarial parasites are known to induce a large range of immunoregulatory mechanisms, including the induction of alternatively activated macrophages and regulatory T cells. These mechanisms are used to evade and down‐modulate the host’s immune system, to support parasite survival. Several reports have focused on some of these mechanisms, in humans and murine models, but the complex immunoregulatory networks associated with filarial infections remain unclear. Recent publications have conferred a role for regulatory T cells in the ability of helminth parasites to modulate human immune responses, such cells promoting the induction of the non‐complement‐fixing immunoglobulin G4 (IgG4). High plasma concentrations of IgG4 have been reported in hypo‐responsive and asymptomatic cases of helminth infection. In both human lymphatic filariasis and onchocerciasis, the asymptomatic infections are characterised by high plasma concentrations of IgG4 (compared with those of IgE) and of the complement‐fixing antibodies IgG1, IgG2 and IgG3. In asymptomatic filarial infection, elevations in IgG4 are also often associated with high worm loads and with high plasma levels of the immunomodulatory interleukin‐10. Here, various aspects of the induction of IgG4 in humans and it roles in the immunomodulation of the human responses to filarial parasites are reviewed.

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Section: Correlation Of Igg4 Levels With the Absence Of Pathology In mentioning

confidence: 86%

Induction of immunoglobulin G4 in human filariasis: an indicator of immunoregulation

Adjobimey¹,

Hoerauf²

2010

Annals of Tropical Medicine & Parasitology

144

148

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“…Sera from individuals harboring microfilaria contained elevated levels of rWmhsp60 [17] and recombinant Wolbachia surface protein specific antibodies [18]. Hence we have produced recombinant Wolbachia surface protein (rWSP) and recombinant Wolbachia heat shock protein 60 (rWmhsp60) in our lab to study their role in filarial pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Wolbachia heat shock protein 60 induces pro-inflammatory cytokines and apoptosis in monocytes in vitro

Vijayan

Sreenivas

Haripriya

et al. 2012

Microbes and Infection

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Recombinant Wolbachia heat shock protein 60 (rWmhsp60) induces gene expression of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in human monocytic cell line THP-1. In addition, it inhibits the phagocytic activity and does not alter the nitric oxide production by differentiated THP-1 macrophages, which corroborates with no significant change in inducible nitric oxide synthase gene expression in rWmhsp60 treated THP-1 monocytes. Further, 24 h stimulation of peripheral blood mononuclear cells from normal individuals by rWmhsp60 reveals that monocytes enter the late apoptotic stage, while lymphocytes do not show apoptosis. Thus these findings suggest that rWmhsp60 may contribute to inflammation mediated monocyte dysfunction in filarial pathogenesis.

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“…Besides this, our recent observation of elevated isotype antibody levels (IgG1 and IgG4 in CP and MF patients) (Shiny et al 2009) against rWSP implies the immunodominant nature of this protein. Hence, it becomes imperative to address in detail the rWSP-induced modulation of immune responses in filarial patients (MF and CP) and endemic normals (EN).…”

Section: Discussionmentioning

confidence: 88%

“…rWSP was expressed and purified as described previously (Shiny et al 2009). Briefly, WSP gene was PCR amplified from B. malayi genomic DNA and cloned in pRSET-A vector for large scale expression of the recombinant protein.…”

Section: Methodsmentioning

confidence: 99%

Recombinant Wolbachia surface protein (WSP)-induced T cell responses in Wuchereria bancrofti infections

et al. 2011

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Human lymphatic filariasis is a debilitating parasitic disease characterized by downregulation of the host’s immune response in asymptomatic carriers along with profound hyperreactivity in chronic patients apart from putatively immune endemic normals. The endosymbiont Wolbachia, a bacterium of filarial nematodes has received much attention as possible chemotherapeutic target and its involvement in disease pathogenesis. The role of recombinant Wolbachia surface protein (rWSP), one of the most abundantly expressed proteins of the endosymbiont, in modulating cell-mediated immune responses in patients harboring Wuchereria bancrofti infections was evaluated in the current study. rWSP-induced lymphoproliferation with peripheral blood mononuclear cells suggested an impaired proliferative response in asymptomatic microfilaremic (MF) and symptomatic chronic pathology (CP) patients compared to endemic normals (EN). This was further supported by a significantly diminished expression of CD69 along with elevated levels of CD127 and CD62L in filarial patients (MF and CP) compared to EN. Further, rWSP induced the expression of regulatory T cell markers CTLA-4 and CD25 along with suppressor cytokines IL-10 and TGF-β in MF and CP patients compared to EN. However, the rWSP-stimulated expression of IFN-γ was diminished significantly in filarial patients compared to endemic normals. Thus, these findings suggest that WSP may also contribute to the suppression of immune responses seen in filarial patients.

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Serum antibody responses to Wolbachia surface protein in patients with human lymphatic filariasis

Cited by 10 publications

References 30 publications

Induction of immunoglobulin G4 in human filariasis: an indicator of immunoregulation

Induction of immunoglobulin G4 in human filariasis: an indicator of immunoregulation

Wolbachia heat shock protein 60 induces pro-inflammatory cytokines and apoptosis in monocytes in vitro

Recombinant Wolbachia surface protein (WSP)-induced T cell responses in Wuchereria bancrofti infections

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