Serum Antioxidative Enzymes Levels and Oxidative Stress Products in Children and Adolescents with Type I Diabetes Mellitus

Abstract

Type I diabetes mellitus (TIDM) is an oxidative stress condition in addition to being a chronic metabolic disease. In this study, our aim is to investigate the activity of antioxidative enzymes and the products of oxidative stress in children and adolescents with TIDM and compare the findings with those in healthy control subjects. Materials and Methods: The study enrolled 41 children and adolescents with TIDM (mean age 11.4±3.3 years; 21 female, 20 male) and 25 healthy subjects (mean age 11.3±3.1 years; 8 female, 17 male) with a similar age and gender distribution. Serum samples were obtained to detect the antioxidative enzymes of paraoxonase (PON), arylesterase (ARE), oxidation degradation products of malondialdehyde (MDA) and also zinc which acts as an antioxidant. Results: We found a significant decrease in PON activity and zinc levels in diabetics compared to the healthy controls (p=0.021; p<0.001, respectively). Zinc was negatively correlated to hemoglobin A1c (r=-0.317, p=0.049). MDA and ARE did not show a significant difference in the TIDM patients compared to the healthy subjects. Conclusion: Zinc level and PON activity were lower in diabetic children and adolescents. Further studies with larger samples are required to confirm their roles in the following and prognosis of TIDM.

Salivary oxidative stress biomarkers in thai adolescents and young adults with type 1 diabetes mellitus: A cross-sectional study

“…Malondialdehyde (MDA) and protein carbonyl (PC) are commonly used as biomarkers in plasma. [ 12 13 14 15 16 ] Some studies reported that plasma levels of MDA or PC in the T1DM group were higher than in the control group,[ 12 13 14 ] while others found no differences between the groups. [ 15 16 ] Total oxidant status (TOS), developed in 2005,[ 17 ] has been used in studies of cancer patients,[ 18 ] periodontal disease,[ 19 ] and T2DM.…”

“…[ 12 13 14 15 16 ] Some studies reported that plasma levels of MDA or PC in the T1DM group were higher than in the control group,[ 12 13 14 ] while others found no differences between the groups. [ 15 16 ] Total oxidant status (TOS), developed in 2005,[ 17 ] has been used in studies of cancer patients,[ 18 ] periodontal disease,[ 19 ] and T2DM. [ 20 ] In T1DM, Aral et al [ 21 ] found that salivary TOS was higher in patients with T1DM at diagnosis compared with a healthy group; however, no significant difference was recorded between patients with T1DM and controls with gingivitis.…”