Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle

Abstract

Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n = 15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n = 15). A quantitative real-time PCR method was used to detect Capripoxvirus (CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection.

Molecular characterization of lumpy skin disease virus (LSDV) emerged in Bangladesh reveals unique genetic features compared to contemporary field strains

“…The Bangladesh LSDVs presented the 12-nucleotide insertion found in the GPCR gene of LSDV KSGP-0240, LSDV Neethling vaccines, and a few historical LSDVs such as the LSDV NI2490 and LSDV Kenya, LSDVs from China and the recombinant LSDVs described in Russia. The presence of this 12-nucleotide insertion makes them different from commonly circulating field LSDVs encountered in Africa, Europe, and the Middle East [ 11 , 16 , 19 , 24 , 40 ]. However, a 27-nucleotide insertion in the EEV glycoprotein gene of Bangladesh isolates, and the RPO30 and GPCR gene trees’ analysis differentiated them from LSDV Neethling derived vaccines.…”

Hematological, biochemical and oxidative stress studies of lumpy skin disease virus infection in cattle

“…Serum ALP was elevated in infected cattle, this phenomenon may have been caused by the effects of inflammation on cells lining and surrounding the biliary ducts; related to the presence of hepatic cholestasis (Abutarbush 2015;Stockham and Scott 2008), which was confirmed by elevated direct bilirubin; or attributable to renal or intestinal infections (Coles 1986). Therefore, elevated AST and ALP in LSDVinfected cattle could be related to viremia-induced hepatic injury (Sevik et al 2016). Serum LDH and CPK were elevated in LSD-infected cattle; based on results indicating that LDH and CPK levels may be elevated due to heart injury, these manifestations may reflect myocardial damage (Marmor et al 1988).…”

Vaccinal prevention of nodular dermatitis in Hereford cattle (clinical and immunological implications)

“…The pathogen is a virus of the genus Capripoxvirus of the family Poxviridae. Clinical evidence is characterized by fever, nodulation on the skin and mucous membranes, corrosion of the respiratory and gastrointestinal tract, and enlargement of the superficial lymph nodes [4][5][6][7]. The vectorborne way of transmission of the pathogen is proved [8].…”