Serum cholesterol determination by gas-liquid chromatography

Blomhoff, J. P.

doi:10.1016/0009-8981(73)90459-2

Cited by 101 publications

(16 citation statements)

References 17 publications

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“…Lipids were extracted by the method of Folch, Lees & Sloane-Stanley (1957). Free and total cholesterol were assayed by gas-liquid chromatography (Blomhoff, 1973); cholesteryl esters were calculated as the difference between total and free cholesterol. Phospholipids were measured as inorganic phosphate (Marinetti, 1962).…”

Section: Methodsmentioning

confidence: 99%

Lecithin—Cholesterol Acyltransferase and the Lipoprotein Abnormalities of Parenchymal Liver Disease

et al. 1979

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1. Detailed studies have been made of the plasma lipoprotein abnormalities in parenchymal liver disease to test the hypothesis that the abnormalities would correlate with plasma lecithin-cholesterol acyltransferase (LCAT) activity. 2. When LCAT was high, very-low-density-lipoproteins (VLDL) were normal in composition and had a normal pre-beta electrophoretic mobility. When LCAT was low, VLDL concentrations were greatly reduced. 3. With high LCAT low-density lipoproteins (LDL) were normal. The LDL particles found with low LCAT activity were of normal size but of abnormal composition, being triglyceride rich and cholesteryl ester poor. Regardless of LCAT activity LDL were present in normal amounts. 4. High-density lipoproteins (HDL) were normal in composition and electron-microscopic appearance when LCAT activity was high. When LCAT activity was low HDL were abnormal in composition and 'stacked discs' were seen on electron microscopy. 5. These results suggest that low LCAT activity may be the cause of at least some of the lipoprotein changes of parenchymal liver disease.

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Section: Methodsmentioning

confidence: 99%

Lecithin—Cholesterol Acyltransferase and the Lipoprotein Abnormalities of Parenchymal Liver Disease

et al. 1979

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“…One tenth of the total volume was taken for cholesterol assay by glc and the rest dried and the radioactivity assayed. The concentrations of free and esterified cholesterol in lymph were measured by the method of Blomhoff [10].…”

Section: Cholesterol-specific Radioactivity Assaysmentioning

confidence: 99%

Effects of starvation and plasma exchange on lecithin: Cholesterol acyltransferase activity and cholesterol efflux in cholesterol-fed pigs

et al. 1984

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The effects of starvation and of plasma exchange with a cholesterol-free substitute on efflux of tissue cholesterol and on lecithin: cholesterol acyltransferase (LCAT) activity in plasma and peripheral lymph were investigated in two pigs fed a cholesterol diet for 3-4 months. The pigs were labelled with i.v. [14C]cholesterol before plasma exchange or starvation. The cholesterol diet increased plasma total cholesterol concentration and LCAT activity in plasma and lymph, but had little effect on the rate of esterification of cholesterol in plasma or lymph. During cholesterol feeding, and when the animals were fed a normal diet, cholesterol esterification rates in plasma and lymph were much lower than the maximum rates achieved when LCAT was saturated with substrate, suggesting that LCAT in normal pig plasma and lymph is not saturated with substrate. Plasma exchange, carried out when the specific activity of tissue cholesterol exceeded that of plasma cholesterol, was followed by a brief rise in the specific activity of plasma cholesterol to a maximum value between the specific activities of muscle and adipose-tissue cholesterol, reflecting the transfer of radioactive cholesterol from tissue to plasma. During the rise in plasma total cholesterol specific activity there were no differences between the specific activities of low-density lipoprotein (LDL) cholesterol and high-density lipoprotein (HDL) cholesterol in plasma or lymph. Starvation had no effect on the plasma-cholesterol specific-activity curve. From about day 14 after labelling, cholesterol-specific activity decreased in the order: tissues greater than lymph greater than plasma. This suggests that the transfer of cholesterol from tissues to plasma was mediated by lipoproteins in the interstitial fluid.

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“…The protein content of fibroblast extracts was determined by the Lowry method after precipitation in 10% trichloroacetic acid. Cholesterol was assayed by the gas-liquid chromatographic method of Blomhoff (25) with 5a-cholestane as standard. Free and esterified cholesterol were separated by thin-layer chromatography before assay.…”

Section: Introductionmentioning

confidence: 99%