Serum concentration of 25 (OH) vitamin D in healthy dogs: factors as age, sex, and diet

Fonseca, F. M. da; Beltrame, Olair Carlos; Seixas, Sandra Vogel; Laskoski, Luciane Maria; Félix, Ananda Portella; Locatelli-Dittrich, Rosângela

doi:10.1007/s00580-020-03111-1

Cited by 2 publications

(7 citation statements)

References 18 publications

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“…Similarly, in a study performed by Fonseca et al. ( 2020 ) no significant difference was observed between vitamin D levels and sex. However, in a study performed by Sharp et al.…”

Section: Discussionmentioning

confidence: 63%

“…( 2002 ) and Fonseca et al. ( 2020 ) on dogs, this relationship was significant. They assumed this difference originates from high levels of growth hormone (GH) in puppies as well as lack of development in their gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 71%

“…As an example, in a study by Fonseca et al. ( 2020 ), serum 25(OH) Vit D concentration in dogs was less than 100 ng/ml, and 25(OH) Vit D levels in most of the dogs ranged between 25 and 100 ng/ml. Other research reported normal levels of 25(OH) Vit D between 9.5 and 249 ng/ml (Selting et al., 2016 ).…”

Section: Discussionmentioning

confidence: 96%

“…Our result differed from the values reported in other studies. As an example, in a study by Fonseca et al (2020), serum 25(OH)…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it has been hypothesised that dogs' skin is inefficient in making vitamin D from ultraviolet radiation. These animals consequently depend solely on a diet to get the vitamin D they need (Fonseca et al., 2020 ). The liver of prey is the main source of vitamin D for carnivores, and food can regularly meet the need for vitamin D in these animals (How et al., 1994 ; Zafalon et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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The effects of age, sex, breed, diet, reproductive status and housing condition on the amounts of 25(OH) vitamin D in the serum of healthy dogs: Reference values

Alizadeh

Ahmadi

Sarchahi

et al. 2022

Veterinary Medicine & Sci

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Background Optimal amount of vitamin D for the proper functioning of the immune system is different from the required vitamin D amount for bones to prevent rickets. However, reports on vitamin D reference values in dogs are minimal, and there is still not enough information regarding the relationship between vitamin D and various variables such as disease, age, breed, diet type, and so on, as well as its relationship with haematological and serum biochemical parameters. Obgectives The present study aimed to determine reference values of 25(OH) Vit D in dogs and its concentration in different groups, categorized based on age, sex, breed, housing conditions, and diet, as well as 25(OH) Vit D relationship with hematology and serum biochemistry parameters. Methods In this study, 90 healthy dogs were selected to determine the reference value of 25 (OH) Vit D of serum after evaluating of their haematological and biochemical parameters to assess their general health. Dogs were divided into different groups according to above‐mentioned variables. Serum 25 (OH) Vit D was subsequently measured by the ELISA method. Results The median concentration of 25 (OH) Vit D was 52.50 ng/ml with minimum and maximum amounts of 14.00 and 155.57 ng/ml, respectively. No significant difference was observed between 25 (OH) Vit D levels in the studied dogs regarding their different age, sex, breed, diet, housing condition, and reproductive status. Serum 25 (OH) Vit D concentration is directly correlated with the number of band neutrophils (p < 0.05). We also witnessed indirect correlations between serum 25 (OH) Vit D levels and the number of blood eosinophils and serum glucose (p < 0.05). Conclusion In the present study age, sex, breed, housing condition and age had no significant effects on the amounts of 25(OH) vitamin D. According to correlations of vitamin D with MCH, band and eosinophil numbers and glucose, vitamin D may have a role in erythropoiesis and leukocytes response and also in energy metabolism in dog.

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“…Similarly, in a study performed by Fonseca et al. ( 2020 ) no significant difference was observed between vitamin D levels and sex. However, in a study performed by Sharp et al.…”

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 96%

“…Our result differed from the values reported in other studies. As an example, in a study by Fonseca et al (2020), serum 25(OH)…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effects of age, sex, breed, diet, reproductive status and housing condition on the amounts of 25(OH) vitamin D in the serum of healthy dogs: Reference values

Alizadeh

Ahmadi

Sarchahi

et al. 2022

Veterinary Medicine & Sci

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A Novel UHPLC–MS/MS Method for the Measurement of 25-Hydroxyvitamin D3 in Canine Serum and Its Application to Healthy Dogs

Bardhi,

Vecchiato,

Sabetti

et al. 2023

Animals

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Several studies have shown the importance of vitamin D3 supplementation in small animals. In dogs, a low vitamin D3 status is associated not only with bone metabolism but also with different kinds of disorders, such as congestive heart failure, gastrointestinal diseases, chronic kidney diseases, and some types of cancer. However, it is crucial to maintain balance and monitor the introduction of this essential nutrient through the diet because over-supplementation can result in toxicity. Due to the clinical importance of assessing the vitamin D3 status in small animal patients, a quick, simple, and highly performing analytical method for its measurement is needed. In this study, we describe the development of a novel liquid chromatography–tandem mass spectrometry method for 25-hydroxyvitamin D3 quantification in canine serum. The approach was successfully validated following current European guidelines, proving excellent linearity (R2 always ≥0.996), accuracy (always within ±13%) and precision (always <10%). The application of the validated approach to samples collected from 40 healthy dogs made possible the definition of a reliable reference interval for 25-hydroxyvitamin D3, the main biomarker of vitamin D3. In addition, variations below 5% in the results obtained quantifying the same samples using a water-based calibration curve demonstrated that a surrogate matrix may be used without affecting data accuracy. Thanks to its simplicity, the proposed technique represents a useful tool for supporting clinical routine and investigating correlations between serum concentrations of this metabolite and multiple diseases. Additionally, it could enable the monitoring of supplementation in small animal patients in veterinary clinical practice.

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Serum concentration of 25 (OH) vitamin D in healthy dogs: factors as age, sex, and diet

Cited by 2 publications

References 18 publications

The effects of age, sex, breed, diet, reproductive status and housing condition on the amounts of 25(OH) vitamin D in the serum of healthy dogs: Reference values

The effects of age, sex, breed, diet, reproductive status and housing condition on the amounts of 25(OH) vitamin D in the serum of healthy dogs: Reference values

A Novel UHPLC–MS/MS Method for the Measurement of 25-Hydroxyvitamin D3 in Canine Serum and Its Application to Healthy Dogs

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