Serum concentrations of squamous-cell carcinoma antigen and tissue polypeptide antigen in the follow-up of patients with vulvar cancer

Hefler, Lukas; Frischmuth, Katrin; Heinze, Georg; Sliutz, Gerhard; Leodolter, Sepp; Reinthaller, Alexander; Kainz, Christian; Tempfer, Clemens

doi:10.1002/(sici)1097-0215(19991008)83:2<167::aid-ijc4>3.0.co;2-e

Cited by 6 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vulvar SCC patients, not only tumours but also metastatic lymph nodes are potential sources of SCCA protein. We think that the raised levels of serum SCCA observed by some researchers in the advanced stages of vulvar carcinoma [36] may result from secondary changes related to the disease progression, such as alterations induced by metastatic cancer cells in their microenvironment and/or an increase in proinflammatory cytokines. These changes create a feedback between local and systemic inflammation, a phenomenon that we have discussed elsewhere [37].…”

Section: Discussionmentioning

confidence: 99%

Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma

et al. 2010

View full text Add to dashboard Cite

Squamous cell carcinoma antigen (SCCA) is expressed in normal squamous cell epithelia and in squamous cell carcinomas (SCC). Two nearly identical genes encode the inhibitory serpins SCCA1 (SERPINB3) and SCCA2 (SERPINB4). Serum levels of SCCA are elevated in patients with benign skin diseases and in patients with SCC. SCCA, used for the monitoring of SCC patients, presents no satisfactory diagnostic specificity. As we have shown previously, the reverse transcription polymerase chain reaction (RT-PCR)-based SCCA messenger RNA (mRNA) testing aimed at detecting disseminated cancer cells may be hampered by the false-positive results due to SCCA expression in activated peripheral blood mononuclear cells (PBMC). The aim of this study was to assess the expression of SCCA at mRNA and protein levels in cultured normal PBMC, compared to that in vulvar SCC (VSCC) samples. High SCCA concentrations were found in vulvar tumours and in metastatic lymph nodes, while negative inguinal lymph nodes from the same patients often presented significantly less SCCA. In normal activated PBMC, the level of SCCA protein was the lowest. At the mRNA level SCCA was detectable in normal PBMC even in cultures with no mitogen stimulation, but only by the nested RT-PCR, contrary to VSCC samples found to be SCCA positive already in one-step PCR. Both SCCA1 and SCCA2 transcripts were present in cultured PBMC; SCCA1 was expressed at a higher level than SCCA2. In conclusion, both SCCA forms are detectable in normal PBMC cultured in vitro. SCCA expression level in normal PBMC is much lower than in the squamous epithelium-derived cells. In VSCC, in addition to tumour itself, metastatic lymph nodes seem also to be a potential source of serum SCCA.

show abstract

Section: Discussionmentioning

confidence: 99%

Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma

et al. 2010

View full text Add to dashboard Cite

show abstract

“…VEGF has angiogenic, mitogenic and vascular permeability enhancing activity specific for endothelial cells, resulting in sprouting of vessels from pre-existing microvessels (VEIKKOLA et al, 2000). VEGF is involved in development and growth of a wide variety of different tumors (HYODO et al, 1998;HEFLER et al, 1999). And plays a role in the formation of distant metastasis (MAEDA et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Fator de crescimento do endotélio vascular no soro e plasma de cães saudáveis

Queiróz¹,

Franchini²,

Neto³

et al. 2014

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

Vascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs.

show abstract

Squamous cell carcinoma antigen serum levels as prognostic parameter in patients with early stage vulvar cancer

Hefler

Sliutz

Leodolter

et al. 2005

Gynecologic Oncology

View full text Add to dashboard Cite

Serum concentrations of squamous-cell carcinoma antigen and tissue polypeptide antigen in the follow-up of patients with vulvar cancer

Cited by 6 publications

References 24 publications

Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma

Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma

Fator de crescimento do endotélio vascular no soro e plasma de cães saudáveis

Squamous cell carcinoma antigen serum levels as prognostic parameter in patients with early stage vulvar cancer

Contact Info

Product

Resources

About