Serum Cytokine Profiling in Cats with Acute Idiopathic Cystitis

Parys, Maciej; Yuzbasiyan‐Gurkan, Vilma; Kruger, John M.

doi:10.1111/jvim.15032

Cited by 23 publications

(39 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For cytokine measurements, citrated plasma was generated by centrifugation of whole blood for 10 min at 1,370 g. After centrifugation, plasma was frozen at −80 • C until cytokine analyses were performed. An antibody-coated microspherebased multiplex feline-specific cytokine immunoassay kit (Milliplex MAP Feline Cytokine/Chemokine Magnetic Bead Panel, Millipore-Sigma, Billerica, MA) was used to measure 19 cytokines as previously described (19)(20)(21)(22). Analytes included in the kit were Fas, FMS-like tyrosine kinase-3 ligand (Flt-3L), granulocyte macrophage-colony stimulating factor (GM-CSF), interferon-γ (IFN-γ), interleukin 1beta (IL-1β), IL-2, plateletderived growth factor-BB (PDGF-BB), IL-12, IL-13, IL-4, IL-6, IL-8, keratinocyte chemoattractant-like (KC-like), stromal cellderived factor 1 (SDF-1), Regulated upon Activation, Normal T cell Expressed, and Secreted (RANTES), stem cell factor (SCF), C-C motif chemokine ligand-2 (CCL-2) [also known as monocyte chemoattractant protein-1 (MCP-1)], tumor-necrosis factor-α (TNF-α), and IL-18.…”

Section: Feline Cytokine Assaysmentioning

confidence: 99%

“…Multiplex assays are able to simultaneously and rapidly measure a wide variety of immunologically active proteins in small volumes of biological samples. Feline-specific multiplex assays have recently been used to measure cytokines in serum and plasma samples from cats affected by heterogeneous diseases, idiopathic cystitis and degenerative joint disease (19)(20)(21)(22), and demonstrate reliable cytokine detection when the blood concentration of the analytes is high.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytokine and Chemokine Profiling in Cats With Sepsis and Septic Shock

et al. 2020

View full text Add to dashboard Cite

Background: Sepsis is a life-threatening condition associated with an exacerbated production of both pro-and anti-inflammatory cytokines that can promote a hyperactive response to infection or induce immunoparalysis. Data regarding the immune response to sepsis in cats are scarce. Establishing the profiles of cytokines and chemokines in feline sepsis to characterize the nature of the immune responses to sepsis might enable individualized treatments to be developed and targeted. Objective: To evaluate the cytokine and chemokine network in cats with sepsis and septic shock, and to investigate the associations of these analytes with disease severity and outcome. Methods: Blood samples prospectively collected at presentation of cats with sepsis and septic shock to two veterinary teaching hospitals were analyzed. Forty healthy cats were included as controls. A 19-plex feline cytokine/chemokine magnetic bead assay system was used to measure analytes in citrated plasma samples. Cytokine concentrations were compared between groups using the Kruskal-Wallis test with Dunn's post-hoc correction for multiple comparisons. Cytokine concentrations were compared between survivors and non-survivors with the Mann-Whitney U test. Odds ratios were calculated using logistic regression. A multivariable logistic regression model for prediction of septic shock was constructed. Results: The study enrolled 35 septic cats. Many cytokines were undetectable in both sick and healthy control cats and were excluded from subsequent analyses. Comparisons of cytokine concentrations among healthy controls, cats with sepsis (n = 12) and cats with septic shock (n = 23) revealed that sick cats (sepsis or septic shock) had significantly higher plasma concentrations of IL-6, IL-8, KC-like, and RANTES compared to healthy controls. The combination of MCP-1, Flt-3L, and IL-12 was predictive of septic shock. None of the cytokines analyzed was predictive of outcome in this study population. Conclusion: Plasma concentrations of IL-6, IL-8, KC-like, and RANTES are increased in cats with sepsis and may play important roles in pathogenesis. Multivariable modeling suggested that analysis of cytokines might aid differentiation of septic shock from sepsis. None of the cytokines analyzed was predictive of outcome. Measurement of these cytokines might enable future studies to better diagnose and characterize feline sepsis and septic shock.

show abstract

Section: Feline Cytokine Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytokine and Chemokine Profiling in Cats With Sepsis and Septic Shock

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Positive/negative or double contrast roentgenograpy and ultrasonography of the abdominal organs allow a detailed study of the bladder to diagnose urocystitis by measuring the thickness of the bladder wall, detecting stones, blood clots, bladder rupture, neoplasia, dislocation and diverticulum (Farrow, 2003;Hostutler et al, 2005;Kealy et al, 2011). The use of cytokines as potentially non-invasive biomarkers for the diagnosis and determination of the effectiveness of treatment of idiopathic urocystitis in cats is suggested (Parys et al, 2018). There is an announcement that the use of immunostimulants of bacterial origin are best suited for treatment of this pathology, in particular Uro-Vaxom ® , as one of its mechanisms of action is the formation of specific immunoglobulins and activation of cellular immunity (Semyonov et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Clinical-diagnostic criteria and peculiarities of treatment of urocystitis in cats

Shulzhenko

Chernenko

Holubyev

et al. 2019

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

The results of a study of cats with signs of urocystitis are presented. The general principles of diagnostics based on clinical, laboratory and additional research methods are established. Clinically acute urocystitis was manifested by disorders of urination – pollakiuria, dysuria, stranguria. Some of the animals showed an increase in body temperature, pain in the bladder and tightness of the walls of the abdominal cavity. According to the results of urine study, it was observed that 43.7% of the cats with urocystitis showed an increase in relative density of the urine, a change in urine pH towards higher alkaline levels, urine residue – erythrocytes, leukocytes and microorganisms were indicated. Impurities of salt and sand were found in 58.4% of the cats. In 31.4% of the cats, urocystitis was a complication of urolithiasis. Early diagnosis, especially differential diagnosis of acute and chronic, secondary urocystitis presents difficulties. Therefore, in cases of suspected inflammatory diseases of the urinary tract, a complex examination using ultrasound of the kidneys, bladder, prostate gland (in males) and the uterus (in females) is recommended.With therapeutic purpose, sick cats (two groups of 8 animals/group) were administered: anti-microbial agent (5% solution of Enrofloxacin), spasmolytic medicinal agent – Riabal®. Animals of the second group were additionally administered the drug Cystocure (Candioli Farmaceutici). According to the instructions, the plant basis of the powder Cystocure is presented by extracts of cranberries and orange peels. These substances provide the main properties of the drug – help in resisting the bacterial colonization of the lower urinary tract and change in urine pH to the acid side. The applied therapy positively influenced the clinical condition of the cats. Their general health was improved, painful sensations during the act of urination disappeared, the quantity and frequency of diuresis normalized. On the seventh day of treatment, according to the results of the study, the urine became transparent, no microorganisms were detected. In cats of the second group, which additionally used the drug Cystocure, the amount of salts and sand decreased significantly, the pH of the urine did not exceed the recommended value of 6.4, in contrast to animals of the first group.

show abstract

“…Cytokine profiling in companion animal medicine has been the subject of a number of recent studies 62 , 64 , 65 . One limitation of such studies to date is the study design to compare cytokine profiles in animals with the designated diagnosis of interest and apparently healthy animals as controls.…”

Section: Discussionmentioning

confidence: 99%

“…For example; CXCL10 and CCL2 plasma concentrations can be combined with circulating levels of IFN-γ to more accurately discriminate between active and latent TB states, whilst the quantification of vascular endothelial growth factor can help differentiate patients with TB pleural effusion from those with neoplastic pleural effusions 57 , 61 . Similar assays have also been used successfully in the study of a limited number of companion animal diseases, including canine lymphoma and feline cystitis 62 – 65 .…”

Section: Introductionmentioning

confidence: 99%

Cytokine and Chemokine Concentrations as Biomarkers of Feline Mycobacteriosis

O’Halloran

McCulloch

Rentoul

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Mycobacteriosis is an emerging zoonotic disease of domestic cats and timely, accurate diagnosis is currently challenging. To identify differential cytokine/chemokine concentrations in serum/plasma of cats, which could be diagnostic biomarkers of infection we analysed plasma/serum from 116 mycobacteria-infected cats, 16 healthy controls and six cats hospitalised for unrelated reasons was analysed using the Milliplex MAP Feline Cytokine Magnetic Bead multiplex assay. Three cytokines; sFAS, IL-13 and IL-4 were reduced while seven; GM-CSF, IL-2, PDGF-BB, IL-8, KC, RANTES and TNF-α were elevated in mycobacteria-infected cats compared to healthy controls. However, IL-8 and KC concentrations were not significantly different from cats hospitalised for other reasons. Elevations in TNF-α and PDGF-BB may have potential to identify M. bovis and M. microti infected cats specifically while GM-CSF, IL-2 and FLT3L were increased in MTBC infected cats. This study demonstrates potential use of feline tuberculosis as a spontaneously occurring model of this significant human disease. Cytokine profiling has clear diagnostic potential for mycobacteriosis of cats and could be used discriminate tuberculous from non-tuberculous disease to rapidly inform on zoonotic risk. Future work should focus on the in-field utility of these findings to establish diagnostic sensitivity and specificity of these markers.

show abstract

Serum Cytokine Profiling in Cats with Acute Idiopathic Cystitis

Cited by 23 publications

References 47 publications

Cytokine and Chemokine Profiling in Cats With Sepsis and Septic Shock

Cytokine and Chemokine Profiling in Cats With Sepsis and Septic Shock

Clinical-diagnostic criteria and peculiarities of treatment of urocystitis in cats

Cytokine and Chemokine Concentrations as Biomarkers of Feline Mycobacteriosis

Contact Info

Product

Resources

About