Serum Diamine Oxidase as a Hemorrhagic Shock Biomarker in a Rabbit Model

Zhao, Liang; Luo, Lin; Jia, Weikun; Xiao, Juan; Huang, Gang; Tian, Geng; Li, Jingwei; Ye, Xiao

doi:10.1371/journal.pone.0102285

Cited by 34 publications

(34 citation statements)

References 42 publications

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“…Thus, this indicates that serum D-Lactate reflects the integrity and maturity of intestinal mucosa [42]. DAO is one of the DAO catalyzed by deaminases, only exists in the villi of the upper small intestine, and its increasing concentrations indicate increased intestinal epithelial permeability or damage to intestinal barrier function [43,44]. Serum endotoxins concentration and DAO activity were both increased with the DFRB level.…”

Section: Discussionmentioning

confidence: 97%

Effects of Increasing Levels of Defatted Rice Bran on Intestinal Physical Barrier and Bacteria in Finishing Pigs

Wang

et al. 2019

Animals

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The aims of this study were to assess the effects of increasing levels of DFRB as a replacement for corns on intestinal physical barrier function and bacteria of finishing pigs. A total of 35 castrated finishing pigs (age: 158.5 ± 2.0 d, initial body weight: 62.9 ± 0.8 kg) were randomly divided into five dietary treatments (seven replicates/treatment) for a 28-day experimental period, i.e., a control diet with basal diet, and four experimental diets in which maize was replaced by 7%, 14%, 21%, and 28% DFRB, respectively. The results showed that serum endotoxins concentration and diamine oxidase (DAO) activity were both increased (linear, p = 0.0004, 0.001, respectively) with DFRB level. However, compared with control group, serum endotoxins concentration and DAO activity were not different in pigs fed with 7% DFRB in the diet. There was a quadratic response in serum D-lactate concentration to the increased DFRB (quadratic, p = 0.021). In the cecum, thickness of the intestinal wall significantly increased with increasing levels of DFRB in the diets (linear, p = 0.033), while crypt depth/thickness of the intestinal wall ratio significantly decreased with increasing level of DFRB in the diets (linear, p = 0.043). In the jejunum, total bacteria, Escherichia coli, and Bifidobacterium all responded quadratically to increasing levels of DFRB in the diets (quadratic, p = 0.003, 0.001, 0.006, respectively). Additionally, there was no difference in Escherichia coli in pigs fed 0%, 7%, and 14% DFRB diets. In the colon, there were quadratic responses in C. perfringens to the increased DFRB (quadratic, p = 0.023). C. perfringens reduced as the DFRB concentration increased from 0% to 14% and then increased. When D-lactate, total bacteria, Escherichia coli, Bifidobacterium, and C. perfringens were considered, the optimal substitution level of DFRB were 12.00%, 11.84%, 7.50%, 8.92%, and 15.92%, respectively. In conclusion, 7% DFRB had a beneficial effect on intestinal wall thickness, Bifidobacterium and C. perfringens, and had no adverse effect on intestinal permeability and Escherichia coli.

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Section: Discussionmentioning

confidence: 97%

Effects of Increasing Levels of Defatted Rice Bran on Intestinal Physical Barrier and Bacteria in Finishing Pigs

Wang

et al. 2019

Animals

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“…The results suggest that there was an increased intestinal permeability in these CRC patients. DAO is an enzyme mainly produced in the small intestine involved in the histamine metabolism [51]. Yee et al has found that an established first-line treatment for patients in T2DM, metformin, inhibits DAO activity [52].…”

Section: Discussionmentioning

confidence: 99%

Alterations of the Predominant Fecal Microbiota and Disruption of the Gut Mucosal Barrier in Patients with Early‐Stage Colorectal Cancer

Liu

Cheng

et al. 2020

BioMed Research International

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Growing evidence indicated that the gut microbiota was the intrinsic and essential component of the cancer microenvironment, which played vital roles in the development and progression of colorectal cancer (CRC). In our present study, we investigated the alterations of fecal abundant microbiota with real-time quantitative PCR and the changes of indicators of gut mucosal barrier from 53 early-stage CRC patients and 45 matched healthy controls. We found that the traditional beneficial bacteria such as Lactobacillus and Bifidobacterium decreased significantly and the carcinogenic bacteria such as Enterobacteriaceae and Fusobacterium nucleatum were significantly increased in CRC patients. We also found gut mucosal barrier dysfunction in CRC patients with increased levels of endotoxin (LPS), D-lactate, and diamine oxidase (DAO). With Pearson’s correlation analysis, D-lactate, LPS, and DAO were correlated negatively with Lactobacillus and Bifidobacterium and positively with Enterobacteriaceae and F. nucleatum. Our present study found dysbiosis of the fecal microbiota and dysfunction of the gut mucosal barrier in patients with early-stage CRC, which implicated that fecal abundant bacteria and gut mucosal barrier indicators could be used as targets to monitor the development and progression of CRC in a noninvasive and dynamic manner.

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“…The DAO is a key marker for monitoring intestinal barrier permeability (33) . When the intestinal mucosal barrier integrity is injured, intestinal DAO will release into the circulation and plasma DAO activity increased (34) . In the present study, plasma DAO activity was significantly increased after piglets being challenged by E. coli K88, indicating that intestinal barrier function was attainted, which was consistent with previous study (35) .…”

Section: Discussionmentioning

confidence: 99%

The involvement of NF-κB/P38 pathways inScutellaria baicalensisextracts attenuating ofEscherichia coliK88-induced acute intestinal injury in weaned piglets

Huang

Wang

et al. 2019

Br J Nutr

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The present study was carried out to evaluate the effect of dietary supplementation of Scutellaria baicalensis extracts (SBE) on intestinal health in terms of morphology, barrier integrity and immune responses in weaned piglets challenged with Escherichia coli K88. A total of seventy-two weaned piglets were assigned into two groups to receive a basal diet without including antibiotic additives or the basal diet supplemented 1000 mg SBE/kg diet for 14 d. On day 15, twelve healthy piglets from each group were selected to expose to oral administration of either 10 ml 1 × 109 colony-forming units of E. coli K88 or the vehicle control. After 48 h of E.coli K88 challenge, blood was sampled, and then all piglets were killed humanely for harvesting jejunal and ileal samples. Dietary supplementation of SBE significantly decreased diarrhoea frequency and improved feed conversion ratio (P < 0·05). SBE supplementation to E.coli K88-challenged piglets improved villous height and villous height/crypt depth (P < 0·05), recovered the protein expression of occludin and zonula occludens-2 in both the jejunum and ileum (P < 0·05), and mitigated the increases in plasma IL-1β, TNF-α, IL-6, IgA and IgG (P < 0·05). Meanwhile, dietary SBE effectively inhibited the stimulation of NF-κB, P38 and TNF-α as well as IL-1β in the small intestine of piglets challenged by E. coli K88 and prevented the activation of NF-κB/P38 signalling pathways (P < 0·05). Collectively, SBE supplementation can potently attenuate diarrhoea in weaning piglets and decrease inflammatory cytokine expressions through inhibiting the NF-κB and P38 signalling pathways.

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Serum Diamine Oxidase as a Hemorrhagic Shock Biomarker in a Rabbit Model

Cited by 34 publications

References 42 publications

Effects of Increasing Levels of Defatted Rice Bran on Intestinal Physical Barrier and Bacteria in Finishing Pigs

Effects of Increasing Levels of Defatted Rice Bran on Intestinal Physical Barrier and Bacteria in Finishing Pigs

Alterations of the Predominant Fecal Microbiota and Disruption of the Gut Mucosal Barrier in Patients with Early‐Stage Colorectal Cancer

The involvement of NF-κB/P38 pathways inScutellaria baicalensisextracts attenuating ofEscherichia coliK88-induced acute intestinal injury in weaned piglets

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