Serum-free culture conditions for serial subculture of undifferentiated PC12 cells

Ohnuma, Kiyoshi; Hayashi, Yohei; Furue, Miho; Kaneko, Kunihiko; Asashima, Makoto

doi:10.1016/j.jneumeth.2005.08.004

Cited by 24 publications

(16 citation statements)

References 33 publications

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“…We applied an established protocol 28 to test the ability of our sensors to measure dopamine discharge from PC12 cells stimulated with extracellular K + (Fig. 4a).…”

Section: Resultsmentioning

confidence: 99%

Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine

et al. 2010

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The development of molecular probes that allow in vivo imaging of neural signaling processes with high temporal and spatial resolution remains challenging. Here we applied directed evolution techniques to create magnetic resonance imaging (MRI) contrast agents sensitive to the neurotransmitter dopamine. The sensors were derived from the heme domain of the bacterial cytochrome P450-BM3 (BM3h). Ligand binding to a site near BM3h’s paramagnetic heme iron led to a drop in MRI signal enhancement and a shift in optical absorbance. Using an absorbance-based screen, we evolved the specificity of BM3h away from its natural ligand and toward dopamine, producing sensors with dissociation constants for dopamine of 3.3–8.9 μM. These molecules were used to image depolarization-triggered neurotransmitter release from PC12 cells and in the brains of live animals. Our results demonstrate the feasibility of molecular-level functional MRI using neural activity–dependent sensors, and our protein engineering approach can be generalized to create probes for other targets.

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“…We applied an established protocol 28 to test the ability of our sensors to measure dopamine discharge from PC12 cells stimulated with extracellular K + (Fig. 4a).…”

Section: Resultsmentioning

confidence: 99%

Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine

et al. 2010

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“…When PC-12 cells were treated with NGF, G1 phase of Interphase was blocked and differentiation was initiated [26]. PC-12 experienced a dramatic phenotypic change when treated with NGF [10]. Percentage of neurite bearing cells of NGF-treated cells at optimum concentration of NGF increased as much as five times compared to non-NGF-treated cells.…”

Section: Resultsmentioning

confidence: 99%

“…PC-12 cell line, derived from transplantable rat pheochromocytoma, has been widely used as neuronal model because it proliferates in growth medium, and stop proliferating and differentiate into neuron-like cells as they respond to NGF [10]. The cell line has been used to study mechanisms of action of neurotoxicants and potential of chemical to alter neurite differentiation [11].…”

Section: Methodsmentioning

confidence: 99%

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Lignosus rhinocerus(Cooke) Ryvarden: A Medicinal Mushroom That Stimulates Neurite Outgrowth in PC-12 Cells

Eik

Naidu

David

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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A national treasure mushroom, Lignosus rhinocerus, has been used to treat variety of ailments by local and indigenous communities in Malaysia. The aim of this study was to investigate the potential of the most valuable part of L. rhinocerus, the sclerotium, on neurite outgrowth activity by using PC-12Adh cell line. Differentiated cells with one thin extension at least double the length of the cell diameter were scored positive. Our results showed that aqueous sclerotium L. rhinocerus extract induced neurite outgrowths of 24.4% and 42.1% at 20 μg/mL (w/v) of aqueous extract alone and a combination of 20 μg/mL (w/v) aqueous extract and 30 ng/mL (w/v) of NGF, respectively. Combination of NGF and sclerotium extract had additive effects and enhanced neurite outgrowth. Neuronal differentiation was demonstrated by indirect immunofluorescence of neurofilament protein. Aqueous sclerotium extract contained neuroactive compounds that stimulated neurite outgrowth in vitro. To our knowledge this is the first report on neurite-stimulating activities of L. rhinocerus.

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“…The rat adrenal pheochromocytoma PC12 cells are widely used as a model neuronal cell line for the study of neurodegenerative diseases, because the behaviors of the cells are similar to neural progenitor cells (Ohnuma et al, 2006). Moreover, PC12 cells have many advantages over primary cultured neuronal cells, such as the cell population has good homology and can be obtained easily (Hong and Liu, 2004).…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects of Abacopterin E from Abacopteris penangiana against oxidative stress-induced neurotoxicity

Lei

Chen

et al. 2011

Journal of Ethnopharmacology

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Serum-free culture conditions for serial subculture of undifferentiated PC12 cells

Cited by 24 publications

References 33 publications

Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine

Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine

Lignosus rhinocerus(Cooke) Ryvarden: A Medicinal Mushroom That Stimulates Neurite Outgrowth in PC-12 Cells

Neuroprotective effects of Abacopterin E from Abacopteris penangiana against oxidative stress-induced neurotoxicity

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