Serum haemolytic complement activities in 11 different MHC (B) typed chicken lines

Parmentier, H.K.; Baelmans, R.; Savelkoul, H.F.J.; Dorny, Pierre; Demey, F.; Berkvens, Dirk

doi:10.1016/j.vetimm.2004.02.009

Cited by 36 publications

(16 citation statements)

References 46 publications

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“…It is important to consider that the mechanisms employed by T. cruzi strains to escape the killing could have evolved differently according to the hosts. Complement system activity is different among humans, opossums, rodents and chickens [50], [51], [52], [53], and their effectiveness in eliminating the parasites could be different. On the other hand, the expression and functionality of complement regulators on T. cruzi surface may be different in the strains, resulting in their different abilities to resist complement killing.…”

Section: Discussionmentioning

confidence: 99%

Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells

Cestari

Ramirez

2010

PLoS ONE

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The complement system is the main arm of the vertebrate innate immune system against pathogen infection. For the protozoan Trypanosoma cruzi, the causative agent of Chagas disease, subverting the complement system and invading the host cells is crucial to succeed in infection. However, little attention has focused on whether the complement system can effectively control T. cruzi infection. To address this question, we decided to analyse: 1) which complement pathways are activated by T. cruzi using strains isolated from different hosts, 2) the capacity of these strains to resist the complement-mediated killing at nearly physiological conditions, and 3) whether the complement system could limit or control T. cruzi invasion of eukaryotic cells. The complement activating molecules C1q, C3, mannan-binding lectin and ficolins bound to all strains analysed; however, C3b and C4b deposition assays revealed that T. cruzi activates mainly the lectin and alternative complement pathways in non-immune human serum. Strikingly, we detected that metacyclic trypomastigotes of some T. cruzi strains were highly susceptible to complement-mediated killing in non-immune serum, while other strains were resistant. Furthermore, the rate of parasite invasion in eukaryotic cells was decreased by non-immune serum. Altogether, these results establish that the complement system recognizes T. cruzi metacyclic trypomastigotes, resulting in killing of susceptible strains. The complement system, therefore, acts as a physiological barrier which resistant strains have to evade for successful host infection.

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Section: Discussionmentioning

confidence: 99%

Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells

Cestari

Ramirez

2010

PLoS ONE

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“…Low complement levels and complement responses as well as speci¢c antibody responses were found in Bolivian ecotypes, compared with various Indian ecotypes (Baelmans et al, in press a,b). Also, di¡erent complement levels were found in commercial lines related to the BG type (Parmentier et al, 2004). In the present study, BG polymorphism was studied in several indigenous chicken populations, using a panel of standard White Leghorn-derived BG-speci¢c alloantisera.…”

Section: Introductionmentioning

confidence: 94%

Serological Screening for MHC (B)-Polymorphism in Indigenous Chickens

Baelmans

Parmentier

Nieuwland

et al. 2005

Tropical Animal Health and Production

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As part of a series of studies to characterize innate and speci¢c immune responses of indigenous chicken lines, birds from Bolivia and India were screened serologically for MHC class IV (BG) polymorphism by direct haemagglutination using haplotype-speci¢c antisera (B2, B4, B12, B13, B14, B15, B19, B21). The sample consisted of 95 Bolivian indigenous chickens and 119 hens from the four most common North Indian`back-yard' chicken lines: Yellow Aseel (AP), Kadaknath (KN), frizzled typed (Ff^) and naked neck (NN). Of all chickens tested, the majority were haplotyped as B2, B15, B19 and B21. Of the Bolivian chickens, 89.5% could be haplotyped: 54.9% were homozygous (including 43.3% B15), and 34.6% were heterozygous (including 15.7% B15). B2-like haplotypes were not found among the Bolivian hens, and only 3.2% of these birds showed homozygous B21-like proteins. Of the Indian hens, MHC (BG)-like proteins could be detected in 60.0% of the AP birds, 6.7% of the KN birds; 40.0% of the Ff^birds; and 10.3% of the NN birds. In these lines, a total of 40.1% (AP), 6.7% (KN), 30.1% (Ff^) and 10.3% (NN) were homozygous for the B-haplotype. Only in the AP line (19.9%), and the Ff^line (9.9%) were heterozygous B-haplotypes types found. The B2 haplotype was found in all Indian chicken lines. Most Indian birds have completely unknown haplotypes, indicating a potentially interesting genetic pool. Subgrouping the Bolivian and Indian indigenous hens into monomorphic BG populations revealed individual di¡erences based on the B-types.

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“…The chicken major histocompatibility complex (MHC) plays an important role in immune response (Heller et al, 1991;Juul-Madsen et al, 2002;Parmentier et al, 2004), disease resistance (Bacon, 1987;Lamont, 1989), productivity (Lamont et al, 1987), and other important economic traits in chickens. It is commonly defined by serological reactions between red blood cell antibodies specific to the MHC class 1 (BF) antigens and the highly polymorphic MHC class 4 (BG) antigens (Meyer et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the major histocompatibility complex region in commercial and noncommercial chicken flocks using the LEI0258 microsatellite marker

Izadi¹,

Ritland

Cheng³

2011

Poultry Science

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Microsatellite marker LEI0258 was used as an indicator to examine the variability of the major histocompatibility complex (MHC) region in 2 commercial layer flocks, 1 experimental layer cross, and 5 noncommercial flocks (used for free-run and free-range meat and egg production). We hypothesized that the populations from noncommercial sources may have more diversity in MHC genes than that in the commercial-source populations. Two related parameters, heterozygosity and the number of alleles harbored by a population, were used to assess the genetic variability. The different combinations of the 22 alleles created 66 genotypes in the 8 chicken populations that were studied. The noncommercial populations, except for the Silkies (SK), harbored more alleles than those in the 2 commercial populations, Lohmann Brown and Lohmann White. The observed heterozygosity of the MHC region was high in all of the populations, except for SK. Considering the 2 parameters we have examined, we can generalize that the intensively selected commercial egg-layer varieties seem to have less genetic variability in their MHC regions compared with that of the noncommercial flocks, which are less intensively selected. The LEI0258 variants can be used as markers to detect most of the MHC haplotypes, but in the different populations the same allele size may not always be associated with the same serologically defined haplotype. The information obtained from this study will be useful for genetic resource conservation and the development of breeding stocks that are suitable for free-range production.

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Serum haemolytic complement activities in 11 different MHC (B) typed chicken lines

Cited by 36 publications

References 46 publications

Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells

Inefficient Complement System Clearance of Trypanosoma cruzi Metacyclic Trypomastigotes Enables Resistant Strains to Invade Eukaryotic Cells

Serological Screening for MHC (B)-Polymorphism in Indigenous Chickens

Genetic diversity of the major histocompatibility complex region in commercial and noncommercial chicken flocks using the LEI0258 microsatellite marker

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