Serum human telomerase reverse transcriptase: a novel biomarker for breast cancer diagnosis

Porika, Mahendar; Tippani, Radhika; Bollam, Sekhar R.; Panuganti, Sree D.; Christopher, Thamidala; Abbagani, Sadanandam

doi:10.1007/s10147-011-0230-6

Cited by 10 publications

(7 citation statements)

References 45 publications

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“…Loss-of-function telomerase gene variants associated with short telomeres were risk factors for sporadic cirrhosis [ 30 ]. The expression of telomerase reverse transcriptase in different parts of human body was found associated with cancer [ 31 , 32 ]. Glucose-methanol-choline oxidoreductase played an important role of cyanohydrin formation and fungal degradation of lignin [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of the Genes Involved in the Fruiting Body Production and Cordycepin Formation ofCordyceps militarisFungus

Zheng¹,

Qiu²,

Han³

2015

Mycobiology

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A mutant library of Cordyceps militaris was constructed by improved Agrobacterium tumefaciens-mediated transformation and screened for degradation features. Six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy T-DNA. T-DNA flanking sequences of these mutants were identified by thermal asymmetric interlaced-PCR approach. ATP-dependent helicase, cytochrome oxidase subunit I and ubiquitin-like activating enzyme were involved in in vitro fruiting body production, serine/threonine phosphatase involved in in vivo fruiting body production, while glucose-methanol-choline oxidoreductase and telomerase reverse transcriptase involved in cordycepin formation. These genes were analyzed by bioinformatics methods, and their molecular function and biology process were speculated by Gene Ontology (GO) analysis. The results provided useful information for the control of culture degeneration in commercial production of C. militaris.

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Section: Discussionmentioning

confidence: 99%

Identification of the Genes Involved in the Fruiting Body Production and Cordycepin Formation ofCordyceps militarisFungus

Zheng¹,

Qiu²,

Han³

2015

Mycobiology

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“…www.oncotarget.com demonstrated in breast cancer [17,37]. Albumin mRNA has also been detected in the peripheral blood of advanced stage (TNM stages III and IV) HCC patients.…”

Section: Oncotarget 2236mentioning

confidence: 96%

Profiling the circulating mRNA transcriptome in human liver disease

et al. 2020

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The human circulation contains cell-free DNA and non-coding microRNA (miRNA). Less is known about the presence of messenger RNA (mRNA). This report profiles the human circulating mRNA transcriptome in people with liver cirrhosis (LC) and hepatocellular carcinoma (HCC) to determine whether mRNA analytes can be used as biomarkers of liver disease. Using RNAseq and RT-qPCR, we investigate circulating mRNA in plasma from HCC and LC patients and demonstrate detection of transcripts representing more than 19,000 different protein coding genes. Remarkably, the circulating mRNA expression levels were similar from person to person over the 21 individuals whose samples were analyzed by RNAseq. Liver derived circulating transcripts such as albumin (ALB), apolipoprotein (APO) A1, A2 & H, serpin A1 & E1, ferritin light chain (FTL) and fibrinogen like 1 (FGL1) were significantly upregulated in HCC patient samples. Higher levels of some of these liver-specific transcripts in the plasma of HCC patients were confirmed by RT-qPCR in another cohort of 20 individuals. Several less abundant circulating transcripts associated with cancer were detected in most HCC samples, but not in healthy subjects. Liver specificity of circulating transcripts was confirmed by investigating their expression in HCC tumor and liver cancer cell lines. Liver specific mRNA sequences in the plasma were predominantly present outside circulating extracellular vesicles. Conclusions: The circulating "mRNA" transcriptome is remarkably consistent in diversity and expression from person to person. Detection of transcripts corresponding to disease selective polypeptides suggests the possibility that circulating mRNA can work as a biomarker analyte for cancer detection.

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“…Recent studies have examined the cell-free mRNA expression of several genes, including TS [ 28 ], β-catenin [ 29 ], hTERT [ 26 , 30 , 31 ], CK19 [ 32 , 33 ], MUC1 [ 34 ], CXCR4 [ 35 ], Bmi-1 [ 35 ], Her-2 [ 36 ] and DKK1 [ 24 ], these studies have focused on the use of cell-free mRNA for early diagnosis, tumor staging and disease monitoring. In contrast, to the best of our knowledge, the present study is the first to examine the potential role of plasma mRNA for predicting docetaxel and pemetrexed chemosensitivity of solid tumors.…”

Section: Discussionmentioning

confidence: 99%

Plasma mRNA expression levels of BRCA1 and TS as potential predictive biomarkers for chemotherapy in gastric cancer

et al. 2014

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ObjectivePersonalized chemotherapy based on predictive biomarkers can maximize efficacy. However, tumor tissue obtained at the time of initial diagnosis will not reflect genetic alterations observed at the time of disease progression. We have examined whether plasma mRNA levels can be a surrogate for tumor levels in predicting chemosensitivity.MethodsIn 150 gastric cancer patients, mRNA levels of BRCA1 and TS were assessed in plasma and paired tumor tissue. The Mann-Whitney U-test was used to compare mRNA expression levels between tumor samples exhibiting in vitro sensitivity or resistance to docetaxel and pemetrexed. All statistical tests were two-sided.ResultsThere were significant correlations between plasma and tumor mRNA levels of BRCA1 (rho = 0.696, P < 0.001) and TS (rho = 0.620, P < 0.001). BRCA1 levels in plasma (docetaxel-sensitive: 1.25; docetaxel-resistant: 0.50, P < 0.001) and tumor (docetaxel-sensitive: 8.81; docetaxel-resistant: 4.88, P < 0.001) were positively associated with docetaxel sensitivity. TS levels in plasma (pemetrexed-sensitive: 0.90; pemetrexed-resistant: 1.82, P < 0.001) and tumor (pemetrexed-sensitive: 6.56; pemetrexed-resistant: 16.69, P < 0.001) were negatively associated with pemetrexed sensitivity.ConclusionsPlasma mRNA expression levels mirror those in the tumor and may have a promising role as potential predictive biomarkers for chemotherapy.Electronic supplementary materialThe online version of this article (doi:10.1186/s12967-014-0355-2) contains supplementary material, which is available to authorized users.

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Serum human telomerase reverse transcriptase: a novel biomarker for breast cancer diagnosis

Abstract: Serum hTERT is useful for diagnosing and assessing the clinical stage of breast cancer and is superior to conventional markers. Therefore, serum hTERT could have a potential application as a novel biomarker for breast cancer diagnosis.

Cited by 10 publications

References 45 publications

Identification of the Genes Involved in the Fruiting Body Production and Cordycepin Formation ofCordyceps militarisFungus

Identification of the Genes Involved in the Fruiting Body Production and Cordycepin Formation ofCordyceps militarisFungus

Profiling the circulating mRNA transcriptome in human liver disease

Plasma mRNA expression levels of BRCA1 and TS as potential predictive biomarkers for chemotherapy in gastric cancer

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